AB130419

Anti-S100A16 antibody

Name: Anti-S100A16 antibody (ab130419) | Abcam
Brand: Abcam Limited
SKU: AB130419

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(8 Publications)

Rabbit Polyclonal S100A16 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human S100A16 aa 1-100.

View Alternative Names

S100F, AAG13, S100A16, Protein S100-A16, Aging-associated gene 13 protein, Protein S100-F, S100 calcium-binding protein A16

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A16 antibody (AB130419)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A16 antibody (AB130419)

ab130419, at a dilution of 1/1000, staining S100A16 in paraffin-embedded Human esophagus tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-S100A16 antibody (AB130419)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-S100A16 antibody (AB130419)

ab130419, at a concentration of 4 µg/ml, staining S100A16 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

Unknown

Western blot - Anti-S100A16 antibody (AB130419)

All lanes:

Western blot - Anti-S100A16 antibody (ab130419) at 1/250 dilution

Lane 1:

RT4 cell lysate

Lane 2:

U251 MG cell lysate

Lane 3:

Human Plasma lysate

Lane 4:

Human Liver lysate

Lane 5:

Human Tonsil lysate

Predicted band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human S100A16 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q96FQ6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" } } }

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Product details

ab130149 is a mono-specific antibody.
The antibody solution should be gently mixed before use.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100A16 also known as S100 calcium-binding protein A16 belongs to the S100 protein family. With a molecular mass of approximately 10.7 kDa S100A16 plays a role in the regulation of calcium homeostasis and cell differentiation. It is expressed in various tissues including the brain heart and adipose tissue. Like other S100 proteins S100A16 possesses two EF-hand motifs which facilitate its ability to bind to calcium ions influencing its structural conformation and function.

Biological function summary

S100A16 is involved in cellular processes such as proliferation differentiation and migration. It participates in the modulation of cytoskeletal dynamics and cell cycle regulation. S100A16 often integrates into multiprotein complexes where it interacts with target proteins to exert its biological effects. Studies show that S100A16 may also contribute to epithelial-mesenchymal transition (EMT) an important event in development and cancer progression.

Pathways

S100A16 functions in signaling pathways linked to calcium-dependent processes. In the MAPK signaling pathway it interacts with proteins like ERK1/2 to regulate cell proliferation and survival. Furthermore S100A16 is also associated with the TGF-beta pathway where its role in EMT becomes significant and involves interactions with TGF-beta-related factors. These pathways underline the importance of S100A16 in cellular homeostasis and developmental processes.

S100A16 is implicated in cancer and metabolic disorders. Its overexpression has been observed in several types of cancer including breast and colorectal cancer where it might contribute to tumor progression and metastasis. S100A16 also interacts with proteins like MMPs which are involved in tissue remodeling and invasion. Additionally S100A16 plays a part in metabolic diseases potentially through its role in adipocyte differentiation and lipid metabolism highlighting its multifaceted contribution to human health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding protein. Binds one calcium ion per monomer (PubMed : 17030513). Can promote differentiation of adipocytes (in vitro) (By similarity). Overexpression in preadipocytes increases their proliferation, enhances adipogenesis and reduces insulin-stimulated glucose uptake (By similarity).

See full target information S100A16

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

ImmunoTargets and therapy 13:571-583 PubMed39478941

2024

Patients with Extensive-Stage Small Cell Lung Cancer Harboring Less Than 4 Metastatic Sites May Benefit from Immune Checkpoint Inhibitor Rechallenge by Reshaping Tumor Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoling Shang,Chenyue Zhang,Yuanyuan Lv,Xiaoxiao Zhang,Kaiyue Guo,Huijuan Li,Haiyong Wang

Polish journal of pathology : official journal of the Polish Society of Pathologists 74:182-193 PubMed37955537

2023

S100A16 cooperates with DEPDC1 to promote the progression and angiogenesis of nephroblastoma through PI3K/Akt/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Geng Geng,Yongtao Xu,Qingfang Li,Qinghao Li,Lili Yuan,Mengyao Dong,Ming Ming

International journal of molecular sciences 24: PubMed37833982

2023

Expression of S100A16 Is Associated with Biological Aggressiveness and Poor Prognosis in Patients with Bladder Cancer Who Underwent Radical Cystectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Katsumata,Kazumasa Matsumoto,Kengo Yanagita,Yuriko Shimizu,Shuhei Hirano,Kazuki Kitajima,Dai Koguchi,Masaomi Ikeda,Yuichi Sato,Masatsugu Iwamura

Oncology research 28:225-236 PubMed31796150

2019

MicroRNA-6884-5p Regulates the Proliferation, Invasion, and EMT of Gastric Cancer Cells by Directly Targeting S100A16.

Applications

Unspecified application

Species

Unspecified reactive species

Huifang Lv,Honglin Hou,Huijun Lei,Caiyun Nie,Beibei Chen,Liangyu Bie,Lili Han,Xiaobing Chen

Bioscience reports 39: PubMed31399502

2019

S100A16-induced adipogenesis is associated with up-regulation of 11 β-hydroxysteroid dehydrogenase type 1 (11β-HSD1).

Applications

Unspecified application

Species

Unspecified reactive species

Rihua Zhang,Jing Bao Kan,Shan Lu,Pei Tong,Jie Yang,Ling Xi,Xiubing Liang,Dongming Su,Dong Li,Yun Liu

Journal of cellular physiology 234:21395-21406 PubMed31069793

2019

S100A16, a novel lipogenesis promoting factor in livers of mice and hepatocytes in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Jingbao Kan,Cuiping Zhao,Shan Lu,Geqian Shen,Jie Yang,Pei Tong,Ling Xi,Rihua Zhang,Xiubin Liang,Dongming Su,Dong Li,Yun Liu

Cancer management and research 11:2449-2456 PubMed31118765

2019

S100A16 regulated by Snail promotes the chemoresistance of nonmuscle invasive bladder cancer through the AKT/Bcl-2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chanjuan Wang,Xi Zhu,Aiwei Li,Shuo Yang,Rui Qiao,Jie Zhang

OncoTargets and therapy 10:5273-5279 PubMed29138580

2017

S100A16, a promising candidate as a prognostic marker for platinum-based adjuvant chemotherapy in resected lung adenocarcinoma.

Applications

IHC-P

Species

Human

Ken Katono,Yuichi Sato,Makoto Kobayashi,Ryo Nagashio,Shinichiro Ryuge,Satoshi Igawa,Masaaki Ichinoe,Yoshiki Murakumo,Makoto Saegusa,Noriyuki Masuda

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-S100A16 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A16 antibody (AB130419)

Immunocytochemistry/ Immunofluorescence - Anti-S100A16 antibody (AB130419)

Western blot - Anti-S100A16 antibody (AB130419)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise