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AB93283

Anti-S100A4 antibody [1F12-1G7]

4

(1 Review)

|

(11 Publications)

Mouse Monoclonal S100A4 antibody. Carrier free. Suitable for ELISA, WB, ICC/IF and reacts with Recombinant full length protein, Mouse, Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human S100A4.

View Alternative Names

CAPL, MTS1, S100A4, Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [1F12-1G7] (AB93283)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [1F12-1G7] (AB93283)

ab93283 at 15μg/ml staining S100A4 in fixed and permeabilised HeLa cells by immunofluorescence.

This image was generated using the ascites version of the product.

Western blot - Anti-S100A4 antibody [1F12-1G7] (AB93283)
  • WB

Unknown

Western blot - Anti-S100A4 antibody [1F12-1G7] (AB93283)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/mL

All lanes:

HeLa cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate

Predicted band size: 12 kDa

true

Western blot - Anti-S100A4 antibody [1F12-1G7] (AB93283)
  • WB

Unknown

Western blot - Anti-S100A4 antibody [1F12-1G7] (AB93283)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/mL

All lanes:

NIH/3T3 cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate

Predicted band size: 12 kDa

true

ELISA - Anti-S100A4 antibody [1F12-1G7] (AB93283)
  • ELISA

Supplier Data

ELISA - Anti-S100A4 antibody [1F12-1G7] (AB93283)

Detection limit for recombinant GST tagged S100A4 is 0.1 ng/ml as a capture antibody.

Western blot - Anti-S100A4 antibody [1F12-1G7] (AB93283)
  • WB

Unknown

Western blot - Anti-S100A4 antibody [1F12-1G7] (AB93283)

Predicted band size for the immunogen in 37.22 kDa.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/mL

All lanes:

immunogen at 0.2 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate

Predicted band size: 12 kDa

Observed band size: 37.22 kDa

true

Western blot - Anti-S100A4 antibody [1F12-1G7] (AB93283)
  • WB

Unknown

Western blot - Anti-S100A4 antibody [1F12-1G7] (AB93283)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/mL

Lane 1:

cell lysate from S100A4 transfected 293T cell line at 25 µg

Lane 2:

cell lysate from non transfected 293T cell line at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate

Predicted band size: 12 kDa

Observed band size: 11.7 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1F12-1G7

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, ICC/IF, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human S100A4.

P26447

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Purified from TCS.
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100A4 also known as metastasin or MTS1 is a protein with a molecular weight of approximately 12 kDa. It functions by binding calcium which induces conformational changes aiding in its interaction with target proteins. S100A4 is part of the S100 family of proteins characterized by two EF-hand calcium-binding motifs. Expression of S100A4 is observed in various cell types including fibroblasts endothelial cells and several tumor cells where it contributes to processes like cell motility invasion and angiogenesis.
Biological function summary

The role of S100A4 extends beyond mere calcium binding; it associates with the cytoskeleton promoting cell structures related to movement and metastasis. It does not act alone but often partners with proteins like myosin IIA to regulate cytoskeletal dynamics. Additionally S100A4 modulates extracellular matrix components and interacts with signaling pathways that control cancer progression making it significant in both normal physiological contexts and pathological states.

Pathways

Functional activities of S100A4 integrate into important pathways involved in cellular movement and metastasis. S100A4 plays roles in the regulation of the Wnt/β-catenin pathway which is pivotal for cell-cell interaction and fate determination. It also interacts with matrix metalloproteinases like MMPs thereby managing processes of tissue invasion and remodeling essential in cancer metastasis. Other pathways influencing cell adhesion and signal transduction may indirectly involve S100A4 through related proteins.

Elevated S100A4 levels have implications in cancer particularly in enhancing metastasis in breast and colorectal cancers. The protein promotes tumor developments by inducing changes in the tumor microenvironment making it a target of interest in oncology. S100A4 also interacts with proteins like p53 and β-catenin which are critical in the pathology of tumors. Moreover S100A4 contributes to fibrotic diseases accentuating tissue remodeling activities by modulating fibroblast behavior and extracellular matrix deposition.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed : 16707441, PubMed : 23752197, PubMed : 30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed : 16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed : 23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed : 26654597, PubMed : 30713770).
See full target information S100A4
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Journal of proteome research 23:3012-3024 PubMed38594816

2024

Analysis of Vascular Smooth Muscle Cells from Thoracic Aortic Aneurysms Reveals DNA Damage and Cell Cycle Arrest as Hallmarks in Bicuspid Aortic Valve Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Ariadna Martin-Blazquez,Marta Martin-Lorenzo,Aranzazu Santiago-Hernandez,Angeles Heredero,Alicia Donado,Juan A Lopez,Miriam Anfaiha-Sanchez,Rocio Ruiz-Jimenez,Vanesa Esteban,Jesus Vazquez,Gonzalo Aldamiz-Echevarria,Gloria Alvarez-Llamas

Science advances 9:eadc9465 PubMed36735787

2023

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Min Fan,Kun Yang,Xiaohui Wang,Linjian Chen,P Spencer Gill,Tuanzhu Ha,Li Liu,Nicole H Lewis,David L Williams,Chuanfu Li

Frontiers in pharmacology 13:930614 PubMed36120348

2022

Ferroptosis induced by iron overload promotes fibrosis in ovarian endometriosis and is related to subpopulations of endometrial stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yanqin Zhang,Xinyu Liu,Mengqi Deng,Chunyu Xu,Yubo Zhang,Di Wu,Fan Tang,Ruiye Yang,Jinwei Miao

Journal of clinical medicine 11: PubMed35628911

2022

Paracrine Interaction of Cholangiocellular Carcinoma with Cancer-Associated Fibroblasts and Schwann Cells Impact Cell Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Jan-Paul Gundlach,Jannik Kerber,Alexander Hendricks,Alexander Bernsmeier,Christine Halske,Christian Röder,Thomas Becker,Christoph Röcken,Felix Braun,Susanne Sebens,Nils Heits

Frontiers in physiology 13:778479 PubMed35309056

2022

Inhibition of PI3K/AKT/mTOR Signalling Pathway Activates Autophagy and Suppresses Peritoneal Fibrosis in the Process of Peritoneal Dialysis.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Jia,Hong Qiu,Lihua Lin,Shun Zhang,Damei Li,Donghua Jin

Frontiers in molecular biosciences 7:192 PubMed32850975

2020

Inhibition of the SET8 Pathway Ameliorates Lung Fibrosis Even Through Fibroblast Dedifferentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Keita Ugai,Shuichi Matsuda,Hideki Mikami,Ayako Shimada,Tomoko Misawa,Hiroyuki Nakamura,Koichiro Tatsumi,Masahiko Hatano,Toshihiko Murayama,Yoshitoshi Kasuya

EBioMedicine 46:274-289 PubMed31395500

2019

A major role of TWEAK/Fn14 axis as a therapeutic target for post-angioplasty restenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Nerea Méndez-Barbero,Carmen Gutierrez-Muñoz,Julio Madrigal-Matute,Pablo Mínguez,Jesús Egido,Jean-Baptiste Michel,Jose L Martín-Ventura,Vanesa Esteban,Luis M Blanco-Colio

Oncology reports 42:1475-1486 PubMed31364740

2019

Upregulated NOX1 expression in gallbladder cancer‑associated fibroblasts predicts a poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Tao Wang,Mohamed Hassan,Kamar Hasan Ansari,Guo-Li Xu,Xin-Ping Li,Yue-Zu Fan

Frontiers in immunology 9:1216 PubMed29910813

2018

S100a4 Is Secreted by Alternatively Activated Alveolar Macrophages and Promotes Activation of Lung Fibroblasts in Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Shinji Ohno,Beatrix Steer,Stephan Klee,Claudia A Staab-Weijnitz,Darcy Wagner,Mareike Lehmann,Tobias Stoeger,Melanie Königshoff,Heiko Adler

International journal of molecular medicine 41:95-106 PubMed29115561

2017

Inhibition of histone deacetylase 1 ameliorates renal tubulointerstitial fibrosis via modulation of inflammation and extracellular matrix gene transcription in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tùng Nguyễn-Thanh,Dal Kim,Sik Lee,Won Kim,Sung Kwang Park,Kyung Pyo Kang
View all publications
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