Mouse Monoclonal S100A4 antibody. Carrier free. Suitable for ELISA, WB, ICC/IF and reacts with Recombinant full length protein, Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Protein S100-A4.
IgG1
Mouse
pH: 7.4
Constituents: 100% PBS
Liquid
Monoclonal
ELISA | WB | ICC/IF | |
---|---|---|---|
Human | Expected | Tested | Tested |
Mouse | Expected | Tested | Expected |
Recombinant full length protein | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
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Species Recombinant full length protein | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Recombinant full length protein | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
Select an associated product type
Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:16707441, PubMed:23752197, PubMed:30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed:23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed:26654597, PubMed:30713770).
CAPL, MTS1, S100A4, MTS1, CAPL, Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4
Mouse Monoclonal S100A4 antibody. Carrier free. Suitable for ELISA, WB, ICC/IF and reacts with Recombinant full length protein, Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Protein S100-A4.
IgG1
Mouse
pH: 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
1F12-1G7
Tissue culture supernatant
kappa
Purified from TCS.
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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This supplementary information is collated from multiple sources and compiled automatically.
S100A4 also known as metastasin or MTS1 is a protein with a molecular weight of approximately 12 kDa. It functions by binding calcium which induces conformational changes aiding in its interaction with target proteins. S100A4 is part of the S100 family of proteins characterized by two EF-hand calcium-binding motifs. Expression of S100A4 is observed in various cell types including fibroblasts endothelial cells and several tumor cells where it contributes to processes like cell motility invasion and angiogenesis.
The role of S100A4 extends beyond mere calcium binding; it associates with the cytoskeleton promoting cell structures related to movement and metastasis. It does not act alone but often partners with proteins like myosin IIA to regulate cytoskeletal dynamics. Additionally S100A4 modulates extracellular matrix components and interacts with signaling pathways that control cancer progression making it significant in both normal physiological contexts and pathological states.
Functional activities of S100A4 integrate into important pathways involved in cellular movement and metastasis. S100A4 plays roles in the regulation of the Wnt/β-catenin pathway which is pivotal for cell-cell interaction and fate determination. It also interacts with matrix metalloproteinases like MMPs thereby managing processes of tissue invasion and remodeling essential in cancer metastasis. Other pathways influencing cell adhesion and signal transduction may indirectly involve S100A4 through related proteins.
Elevated S100A4 levels have implications in cancer particularly in enhancing metastasis in breast and colorectal cancers. The protein promotes tumor developments by inducing changes in the tumor microenvironment making it a target of interest in oncology. S100A4 also interacts with proteins like p53 and β-catenin which are critical in the pathology of tumors. Moreover S100A4 contributes to fibrotic diseases accentuating tissue remodeling activities by modulating fibroblast behavior and extracellular matrix deposition.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Predicted band size for the immunogen in 37.22 kDa.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/mL
All lanes: immunogen at 0.2 µg
All lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 37.22 kDa
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/mL
All lanes: HeLa cell lysate at 50 µg
All lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDa
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/mL
All lanes: NIH/3T3 cell lysate at 50 µg
All lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDa
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/mL
Lane 1: cell lysate from S100A4 transfected 293T cell line at 25 µg
Lane 2: cell lysate from non transfected 293T cell line at 25 µg
All lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 11.7 kDa
ab93283 at 15μg/ml staining S100A4 in fixed and permeabilised HeLa cells by immunofluorescence.
This image was generated using the ascites version of the product.
Detection limit for recombinant GST tagged S100A4 is 0.1 ng/ml as a capture antibody.
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