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Mouse Monoclonal S100A4 antibody. Carrier free. Suitable for ELISA, WB, ICC/IF and reacts with Recombinant full length protein, Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Protein S100-A4.


Images

Western blot - Anti-S100A4 antibody [1F12-1G7] (AB93283), expandable thumbnail
  • Western blot - Anti-S100A4 antibody [1F12-1G7] (AB93283), expandable thumbnail
  • Western blot - Anti-S100A4 antibody [1F12-1G7] (AB93283), expandable thumbnail
  • Western blot - Anti-S100A4 antibody [1F12-1G7] (AB93283), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [1F12-1G7] (AB93283), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human Protein S100-A4. Database link P26447

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWBICC/IF
Human
Expected
Tested
Tested
Mouse
Expected
Tested
Expected
Recombinant full length protein
Tested
Not recommended
Not recommended

Tested
Tested

Species

Recombinant full length protein

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:16707441, PubMed:23752197, PubMed:30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed:23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed:26654597, PubMed:30713770).

Alternative names

Recommended products

Mouse Monoclonal S100A4 antibody. Carrier free. Suitable for ELISA, WB, ICC/IF and reacts with Recombinant full length protein, Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Protein S100-A4.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human Protein S100-A4. Database link P26447
Carrier free

Yes

Clone number

1F12-1G7

Purity

Tissue culture supernatant

Light chain type

kappa

Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

S100A4 also known as metastasin or MTS1 is a protein with a molecular weight of approximately 12 kDa. It functions by binding calcium which induces conformational changes aiding in its interaction with target proteins. S100A4 is part of the S100 family of proteins characterized by two EF-hand calcium-binding motifs. Expression of S100A4 is observed in various cell types including fibroblasts endothelial cells and several tumor cells where it contributes to processes like cell motility invasion and angiogenesis.

Biological function summary

The role of S100A4 extends beyond mere calcium binding; it associates with the cytoskeleton promoting cell structures related to movement and metastasis. It does not act alone but often partners with proteins like myosin IIA to regulate cytoskeletal dynamics. Additionally S100A4 modulates extracellular matrix components and interacts with signaling pathways that control cancer progression making it significant in both normal physiological contexts and pathological states.

Pathways

Functional activities of S100A4 integrate into important pathways involved in cellular movement and metastasis. S100A4 plays roles in the regulation of the Wnt/β-catenin pathway which is pivotal for cell-cell interaction and fate determination. It also interacts with matrix metalloproteinases like MMPs thereby managing processes of tissue invasion and remodeling essential in cancer metastasis. Other pathways influencing cell adhesion and signal transduction may indirectly involve S100A4 through related proteins.

Associated diseases and disorders

Elevated S100A4 levels have implications in cancer particularly in enhancing metastasis in breast and colorectal cancers. The protein promotes tumor developments by inducing changes in the tumor microenvironment making it a target of interest in oncology. S100A4 also interacts with proteins like p53 and β-catenin which are critical in the pathology of tumors. Moreover S100A4 contributes to fibrotic diseases accentuating tissue remodeling activities by modulating fibroblast behavior and extracellular matrix deposition.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283), expandable thumbnail

    Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)

    Predicted band size for the immunogen in 37.22 kDa.

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/mL

    All lanes: immunogen at 0.2 µg

    Secondary

    All lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Developed using the ECL technique.

    Predicted band size: 12 kDa

    Observed band size: 37.22 kDa

  • Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283), expandable thumbnail

    Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/mL

    All lanes: HeLa cell lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Developed using the ECL technique.

    Predicted band size: 12 kDa

  • Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283), expandable thumbnail

    Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/mL

    All lanes: NIH/3T3 cell lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Developed using the ECL technique.

    Predicted band size: 12 kDa

  • Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283), expandable thumbnail

    Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/mL

    Lane 1: cell lysate from S100A4 transfected 293T cell line at 25 µg

    Lane 2: cell lysate from non transfected 293T cell line at 25 µg

    Secondary

    All lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Developed using the ECL technique.

    Predicted band size: 12 kDa

    Observed band size: 11.7 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [1F12-1G7] (ab93283), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [1F12-1G7] (ab93283)

    ab93283 at 15μg/ml staining S100A4 in fixed and permeabilised HeLa cells by immunofluorescence.

    This image was generated using the ascites version of the product.

  • ELISA - Anti-S100A4 antibody [1F12-1G7] (ab93283), expandable thumbnail

    ELISA - Anti-S100A4 antibody [1F12-1G7] (ab93283)

    Detection limit for recombinant GST tagged S100A4 is 0.1 ng/ml as a capture antibody.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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