AB58597

Anti-S100A4 antibody

Name: Anti-S100A4 antibody (ab58597) | Abcam
Brand: Abcam Limited
SKU: AB58597
Price: 480 USD
Availability: InStock

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(5 Publications)

Goat Polyclonal S100A4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human S100A4 aa 50-100.

View Alternative Names

CAPL, MTS1, S100A4, Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4

2 Images

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Western blot - Anti-S100A4 antibody (AB58597)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-S100A4 antibody (ab58597) at 0.03 µg/mL

All lanes:

Peripheral blood mononuclear cell lysate (in RIPA buffer) at 35 µg

Predicted band size: 12 kDa

Observed band size: 13 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody (AB58597)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody (AB58597)

ab58597 at a concentration of 2μg/ml staining S100A4 in Human small intestine tissue sections by immunohistochemistry (paraffin-embedded). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human S100A4 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P26447

Specificity

Both variants (NP_002952.1; NP_062427.1) represent identical protein.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100A4 also known as metastasin or MTS1 is a protein with a molecular weight of approximately 12 kDa. It functions by binding calcium which induces conformational changes aiding in its interaction with target proteins. S100A4 is part of the S100 family of proteins characterized by two EF-hand calcium-binding motifs. Expression of S100A4 is observed in various cell types including fibroblasts endothelial cells and several tumor cells where it contributes to processes like cell motility invasion and angiogenesis.

Biological function summary

The role of S100A4 extends beyond mere calcium binding; it associates with the cytoskeleton promoting cell structures related to movement and metastasis. It does not act alone but often partners with proteins like myosin IIA to regulate cytoskeletal dynamics. Additionally S100A4 modulates extracellular matrix components and interacts with signaling pathways that control cancer progression making it significant in both normal physiological contexts and pathological states.

Pathways

Functional activities of S100A4 integrate into important pathways involved in cellular movement and metastasis. S100A4 plays roles in the regulation of the Wnt/β-catenin pathway which is pivotal for cell-cell interaction and fate determination. It also interacts with matrix metalloproteinases like MMPs thereby managing processes of tissue invasion and remodeling essential in cancer metastasis. Other pathways influencing cell adhesion and signal transduction may indirectly involve S100A4 through related proteins.

Elevated S100A4 levels have implications in cancer particularly in enhancing metastasis in breast and colorectal cancers. The protein promotes tumor developments by inducing changes in the tumor microenvironment making it a target of interest in oncology. S100A4 also interacts with proteins like p53 and β-catenin which are critical in the pathology of tumors. Moreover S100A4 contributes to fibrotic diseases accentuating tissue remodeling activities by modulating fibroblast behavior and extracellular matrix deposition.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed : 16707441, PubMed : 23752197, PubMed : 30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed : 16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed : 23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed : 26654597, PubMed : 30713770).

See full target information S100A4

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3020 PubMed37230982

2023

Wound infiltrating adipocytes are not myofibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Shruthi Kalgudde Gopal,Ruoxuan Dai,Ania Maria Stefanska,Meshal Ansari,Jiakuan Zhao,Pushkar Ramesh,Johannes W Bagnoli,Donovan Correa-Gallegos,Yue Lin,Simon Christ,Ilias Angelidis,Valerio Lupperger,Carsten Marr,Lindsay C Davies,Wolfgang Enard,Hans-Günther Machens,Herbert B Schiller,Dongsheng Jiang,Yuval Rinkevich

Annals of translational medicine 10:930 PubMed36172090

2022

Rutaecarpine prevents the malignant biological properties of breast cancer cells by the miR-149-3p/S100A4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Xiong,Chao Xiong,Peng Li,Xuehua Shan

Nature communications 11:5653 PubMed33159076

2020

Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Jiang,Simon Christ,Donovan Correa-Gallegos,Pushkar Ramesh,Shruthi Kalgudde Gopal,Juliane Wannemacher,Christoph H Mayr,Valerio Lupperger,Qing Yu,Haifeng Ye,Martin Mück-Häusl,Vijayanand Rajendran,Li Wan,Juan Liu,Ursula Mirastschijski,Thomas Volz,Carsten Marr,Herbert B Schiller,Yuval Rinkevich

JCI insight 5: PubMed32814713

2020

CD47 prevents the elimination of diseased fibroblasts in scleroderma.

Applications

Unspecified application

Species

Unspecified reactive species

Tristan Lerbs,Lu Cui,Megan E King,Tim Chai,Claire Muscat,Lorinda Chung,Ryanne Brown,Kerri Rieger,Tyler Shibata,Gerlinde Wernig

Molecular & cellular proteomics : MCP 12:3778-92 PubMed24037664

2013

Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Kati Rasanen,Sira Sriswasdi,Alexander Valiga,Hsin-Yao Tang,Gao Zhang,Michela Perego,Rajasekharan Somasundaram,Ling Li,Kaye Speicher,Andres J Klein-Szanto,Devraj Basu,Anil K Rustgi,David W Speicher,Meenhard Herlyn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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