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AB281227

Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal S100A4 antibody. Carrier free. Suitable for WB, sELISA and reacts with Human samples.

View Alternative Names

CAPL, MTS1, S100A4, Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4

2 Images
Western blot - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (Capture) (AB281227)
  • WB

Lab

Western blot - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (Capture) (AB281227)

This data was developed using the same antibody clone in a different buffer formulation (ab124805).

Lanes 1 - 2 : Merged signal (red and green). Green - ab124805 observed at 11 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab124805 was shown to react with S100A4 in wild-type HeLa cells in Western blot with loss of signal observed in S100A4 knockout cell line ab265709 (S100A4 knockout cell lysate ab257046). Wild-type HeLa and S100A4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab124805 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-S100A4 antibody [EPR2761(2)] (<a href='/en-us/products/primary-antibodies/s100a4-antibody-epr27612-ab124805'>ab124805</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human S100A4 knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-s100a4-knockout-hela-cell-lysate-ab257046'>ab257046</a>) at 20 µg

Predicted band size: 12 kDa

Observed band size: 11 kDa

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Sandwich ELISA - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (Capture) (AB281227)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (Capture) (AB281227)

Example of Human S100A4 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Carrier free

    Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free

  • Unconjugated

    Anti-S100A4 antibody [EPR2761(2)]

  • 660 APC

    APC Anti-S100A4 antibody [EPR2761(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-S100A4 antibody [EPR2761(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-S100A4 antibody [EPR2761(2)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-S100A4 antibody [EPR2761(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-S100A4 antibody [EPR2761(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-S100A4 antibody [EPR2761(2)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-S100A4 antibody [EPR2761(2)]

  • HRP

    HRP Anti-S100A4 antibody [EPR2761(2)]

  • 578 PE

    PE Anti-S100A4 antibody [EPR2761(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2761(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab281227 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human S100A4. The recommended pair for sandwich ELISA is:
Capture: ab281227, Human S100A4 Capture Antibody (unconjugated)
Detector: ab281077, Human S100A4 Detector Antibody (unconjugated)
The reference range value is 15.6 - 1000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100A4 also known as metastasin or MTS1 is a protein with a molecular weight of approximately 12 kDa. It functions by binding calcium which induces conformational changes aiding in its interaction with target proteins. S100A4 is part of the S100 family of proteins characterized by two EF-hand calcium-binding motifs. Expression of S100A4 is observed in various cell types including fibroblasts endothelial cells and several tumor cells where it contributes to processes like cell motility invasion and angiogenesis.
Biological function summary

The role of S100A4 extends beyond mere calcium binding; it associates with the cytoskeleton promoting cell structures related to movement and metastasis. It does not act alone but often partners with proteins like myosin IIA to regulate cytoskeletal dynamics. Additionally S100A4 modulates extracellular matrix components and interacts with signaling pathways that control cancer progression making it significant in both normal physiological contexts and pathological states.

Pathways

Functional activities of S100A4 integrate into important pathways involved in cellular movement and metastasis. S100A4 plays roles in the regulation of the Wnt/β-catenin pathway which is pivotal for cell-cell interaction and fate determination. It also interacts with matrix metalloproteinases like MMPs thereby managing processes of tissue invasion and remodeling essential in cancer metastasis. Other pathways influencing cell adhesion and signal transduction may indirectly involve S100A4 through related proteins.

Elevated S100A4 levels have implications in cancer particularly in enhancing metastasis in breast and colorectal cancers. The protein promotes tumor developments by inducing changes in the tumor microenvironment making it a target of interest in oncology. S100A4 also interacts with proteins like p53 and β-catenin which are critical in the pathology of tumors. Moreover S100A4 contributes to fibrotic diseases accentuating tissue remodeling activities by modulating fibroblast behavior and extracellular matrix deposition.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed : 16707441, PubMed : 23752197, PubMed : 30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed : 16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Also modulates the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed : 23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed : 26654597, PubMed : 30713770).
See full target information S100A4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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