Anti-S100A4 antibody [S100A4/1481]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [S100A4/1481] (AB218511)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human placenta tissue, labeling S100A4 using ab218511 at 1 μg/mL.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-S100A4 antibody [S100A4/1481] (AB218511)

Flow Cytometric Analysis of human T98G cells using ab218511 at 1 ug/ml, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

• WB

Lab

Western blot - Anti-S100A4 antibody [S100A4/1481] (AB218511)

Lanes 1 - 4 : Merged signal (red and green). Green - ab218511 observed at 12 kDa. Red - loading control ab181602 observed at 37 kDa.

ab218511 Anti-S100A4 antibody [S100A4/1481] was shown to specifically react with S100A4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261758 (knockout cell lysate ab257045) was used. Wild-type and S100A4 knockout samples were subjected to SDS-PAGE. ab218511 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

S100A4 knockout HeLa cell lysate at 20 µg

Predicted band size: 12 kDa

Observed band size: 12 kDa

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• WB

Lab

Western blot - Anti-S100A4 antibody [S100A4/1481] (AB218511)

Lanes 1 - 2 : Merged signal (red and green). Green - ab218511 observed at 11 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

ab218511 was shown to react with S100A4 in wild-type HeLa cells in Western blot with loss of signal observed in S100A4 knockout cell line ab265709 (S100A4 knockout cell lysate ab257046). Wild-type HeLa and S100A4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab218511 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511) at 0.5 µg/mL

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

S100A4 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human S100A4 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-s100a4-knockout-hela-cell-line-ab265709'>ab265709</a>)

Predicted band size: 12 kDa

Observed band size: 11 kDa

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• WB

Supplier Data

Western blot - Anti-S100A4 antibody [S100A4/1481] (AB218511)

All lanes:

Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511) at 1 µg/mL

Lane 1:

HeLa cell lysate

Lane 2:

A549 cell lysate

Lane 3:

A375 cell lysate

Predicted band size: 12 kDa

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• WB

Lab

Western blot - Anti-S100A4 antibody [S100A4/1481] (AB218511)

Lanes 1 - 4 : Merged signal (red and green). Green - ab218511 observed at 12 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab218511 was shown to specifically react with S100A4 in wild-type A549 cells as signal was lost in S100A4 knockout cells. Wild-type and S100A4 knockout samples were subjected to SDS-PAGE. ab218511 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511) at 1 µg/mL

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

S100A4 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human S100A4 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-s100a4-knockout-a549-cell-line-ab261865'>ab261865</a>)

Lane 3:

A-375 (Human malignant melanoma cell line) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 12 kDa

Observed band size: 12 kDa

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• Protein Array

Unknown

Protein Array - Anti-S100A4 antibody [S100A4/1481] (AB218511)

ab218511 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.