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Mouse Monoclonal S100A4 antibody. Suitable for ICC, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human S100A4.

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Images

Western blot - Anti-S100A4 antibody [S100A4/1482] (AB218512), expandable thumbnail
  • Immunocytochemistry - Anti-S100A4 antibody [S100A4/1482] (AB218512), expandable thumbnail
  • Flow Cytometry - Anti-S100A4 antibody [S100A4/1482] (AB218512), expandable thumbnail
  • Western blot - Anti-S100A4 antibody [S100A4/1482] (AB218512), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [S100A4/1482] (AB218512), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human S100A4. The exact immunogen used to generate this antibody is proprietary information. Database link P26447

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow CytWBIHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg for 106 Cells
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

6 products for Alternative Product

Target data

Function

Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:16707441, PubMed:23752197, PubMed:30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed:23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed:26654597, PubMed:30713770).

Alternative names

CAPL, MTS1, S100A4, Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4

Recommended products

Mouse Monoclonal S100A4 antibody. Suitable for ICC, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human S100A4.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human S100A4. The exact immunogen used to generate this antibody is proprietary information. Database link P26447
Clone number
S100A4/1482
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...
Purification notes

ab218512 is purified from Bioreactor Concentrate by Protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

S100A4 also known as metastasin or MTS1 is a protein with a molecular weight of approximately 12 kDa. It functions by binding calcium which induces conformational changes aiding in its interaction with target proteins. S100A4 is part of the S100 family of proteins characterized by two EF-hand calcium-binding motifs. Expression of S100A4 is observed in various cell types including fibroblasts endothelial cells and several tumor cells where it contributes to processes like cell motility invasion and angiogenesis.

Biological function summary

The role of S100A4 extends beyond mere calcium binding; it associates with the cytoskeleton promoting cell structures related to movement and metastasis. It does not act alone but often partners with proteins like myosin IIA to regulate cytoskeletal dynamics. Additionally S100A4 modulates extracellular matrix components and interacts with signaling pathways that control cancer progression making it significant in both normal physiological contexts and pathological states.

Pathways

Functional activities of S100A4 integrate into important pathways involved in cellular movement and metastasis. S100A4 plays roles in the regulation of the Wnt/β-catenin pathway which is pivotal for cell-cell interaction and fate determination. It also interacts with matrix metalloproteinases like MMPs thereby managing processes of tissue invasion and remodeling essential in cancer metastasis. Other pathways influencing cell adhesion and signal transduction may indirectly involve S100A4 through related proteins.

Associated diseases and disorders

Elevated S100A4 levels have implications in cancer particularly in enhancing metastasis in breast and colorectal cancers. The protein promotes tumor developments by inducing changes in the tumor microenvironment making it a target of interest in oncology. S100A4 also interacts with proteins like p53 and β-catenin which are critical in the pathology of tumors. Moreover S100A4 contributes to fibrotic diseases accentuating tissue remodeling activities by modulating fibroblast behavior and extracellular matrix deposition.

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6 product images

  • Western blot - Anti-S100A4 antibody [S100A4/1482] (ab218512), expandable thumbnail

    Western blot - Anti-S100A4 antibody [S100A4/1482] (ab218512)

    Lanes 1 - 2: Merged signal (red and green). Green - ab218512 observed at 11 kDa. Red - loading control Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

    ab218512 was shown to react with S100A4 in wild-type HeLa cells in Western blot with loss of signal observed in S100A4 knockout cell line Human S100A4 knockout HeLa cell line ab265709 (S100A4 knockout cell lysate Human S100A4 knockout HeLa cell lysate ab257046). Wild-type HeLa and S100A4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab218512 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-S100A4 antibody [S100A4/1482] (ab218512) at 0.5 µg/mL

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: S100A4 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human S100A4 knockout HeLa cell line (Human S100A4 knockout HeLa cell line ab265709)

    Performed under reducing conditions.

    Predicted band size: 12 kDa

    Observed band size: 11 kDa

  • Immunocytochemistry - Anti-S100A4 antibody [S100A4/1482] (ab218512), expandable thumbnail

    Immunocytochemistry - Anti-S100A4 antibody [S100A4/1482] (ab218512)

    Immunocytochemistry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling S100A4 with ab218512 followed by goat anti-mouse IgG-CF488 (green). Reddot is used to label the nuclei Red.

  • Flow Cytometry - Anti-S100A4 antibody [S100A4/1482] (ab218512), expandable thumbnail

    Flow Cytometry - Anti-S100A4 antibody [S100A4/1482] (ab218512)

    Flow Cytometry analysis of T98G (human brain glioblastoma multiforme cell line) cells labeling S100A4 with ab218512 followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

  • Western blot - Anti-S100A4 antibody [S100A4/1482] (ab218512), expandable thumbnail

    Western blot - Anti-S100A4 antibody [S100A4/1482] (ab218512)

    Lanes 1 - 4: Merged signal (red and green). Green - ab218512 observed at 12 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.

    ab218512 was shown to specifically react with S100A4 in wild-type A549 cells as signal was lost in S100A4 knockout cells. Wild-type and S100A4 knockout samples were subjected to SDS-PAGE. ab218512 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-S100A4 antibody [S100A4/1482] (ab218512) at 1 µg/mL

    Lane 1: Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: Human S100A4 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: Western blot - Human S100A4 knockout A549 cell line (Human S100A4 knockout A549 cell line ab261865)

    Lane 3: A-375 (Human malignant melanoma cell line) whole cell lysate at 20 µg

    Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Predicted band size: 12 kDa

    Observed band size: 12 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [S100A4/1482] (ab218512), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [S100A4/1482] (ab218512)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling S100A4 with ab218512 at 1 μg/mL.

  • Western blot - Anti-S100A4 antibody [S100A4/1482] (ab218512), expandable thumbnail

    Western blot - Anti-S100A4 antibody [S100A4/1482] (ab218512)

    All lanes: Western blot - Anti-S100A4 antibody [S100A4/1482] (ab218512) at 1 µg/mL

    Lane 1: HeLa cell lysate

    Lane 2: A549 cell lysate

    Lane 3: A375 cell lysate

    Predicted band size: 12 kDa

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