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AB231215

Anti-S100A8 antibody

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Rabbit Polyclonal MRP8 antibody. Suitable for WB and reacts with Pig, Human samples.
5 Images
Western blot - Anti-S100A8 antibody (AB231215)
  • WB

Supplier Data

Western blot - Anti-S100A8 antibody (AB231215)

All lanes:

Western blot - Anti-S100A8 antibody (ab231215) at 2 µg/mL

All lanes:

Pig spleen lysate

Predicted band size: 10 kDa

false

Western blot - Anti-S100A8 antibody (AB231215)
  • WB

Supplier Data

Western blot - Anti-S100A8 antibody (AB231215)

All lanes:

Western blot - Anti-S100A8 antibody (ab231215) at 2 µg/mL

All lanes:

Pig lymph node lysate

Predicted band size: 10 kDa

false

Western blot - Anti-S100A8 antibody (AB231215)
  • WB

Supplier Data

Western blot - Anti-S100A8 antibody (AB231215)

All lanes:

Western blot - Anti-S100A8 antibody (ab231215) at 2 µg/mL

All lanes:

Pig lung lysate

Predicted band size: 10 kDa

false

Western blot - Anti-S100A8 antibody (AB231215)
  • WB

Supplier Data

Western blot - Anti-S100A8 antibody (AB231215)

All lanes:

Western blot - Anti-S100A8 antibody (ab231215) at 2 µg/mL

All lanes:

Human leukocyte lysate

Predicted band size: 10 kDa

false

Western blot - Anti-S100A8 antibody (AB231215)
  • WB

Supplier Data

Western blot - Anti-S100A8 antibody (AB231215)

All lanes:

Western blot - Anti-S100A8 antibody (ab231215) at 2 µg/mL

All lanes:

Recombinant pig MRP8 protein

Predicted band size: 10 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100A8 also known as calprotectin or MRP8 is a calcium-binding protein with a molecular weight of around 10.8 kDa. It is a member of the S100 protein family and is found expressed in myeloid cells such as neutrophils and monocytes. This protein often forms a heterodimer complex with S100A9 together referred to as calprotectin which plays a critical role in the immune response. Researchers frequently utilize calprotectin fecal ELISA MRP8 ELISA or calprotectin serum immunoassay to measure its levels in various biological samples.
Biological function summary

The S100A8/S100A9 complex modulates inflammatory processes and immune responses. It acts as a pro-inflammatory mediator and is associated with leukocyte recruitment to sites of inflammation. This complex plays an important role in protecting cells from infections by inhibiting bacterial growth through sequestration of nutrient metals showcasing antimicrobial properties. Calprotectin significantly impacts immune responses and has become an interesting target for calprotectin ELISA kits to quantify its presence in plasma and other bodily fluids indicating inflammation.

Pathways

S100A8 and its partner S100A9 are involved in toll-like receptor and receptor for advanced glycation end-products (RAGE) signaling pathways. These pathways are key in mediating inflammatory responses and linking to innate immunity. The calgranulin A ELISA is often used to evaluate their involvement in these pathways helping to elucidate interactions with other proteins like calmodulin that play a role in cellular regulation processes.

S100A8 is strongly associated with inflammatory conditions such as rheumatoid arthritis and inflammatory bowel disease. Its elevated expression levels serve as a biomarker for these conditions highlighting its potential for diagnostic use via calprotectin ELISA kits. S100A8's relationship with S100A9 supports its connection to these diseases reflecting the complex's significant presence and functional role in chronic inflammation and immune dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information S100A8
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