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AB92331

Anti-S100A8 antibody [EPR3554]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • 20ul selling size
  • What is this?

4

(7 Reviews)

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(70 Publications)

Anti-S100A8 antibody [EPR3554] (ab92331) is a rabbit monoclonal antibody detecting S100A8 in Western Blot, IP, IHC-P. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications
- Trusted since 2010

View Alternative Names

CAGA, CFAG, MRP8, S100A8, Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, Urinary stone protein band A, MRP-8, p8

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (AB92331)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (AB92331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labeling S100A8 with purified ab92331 at 1/500.

Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0. ab97051 an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (AB92331)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (AB92331)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labelling S100A8 with ab92331 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101.

Positive staining on human spleen.

The section was incubated with ab92331 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry - Anti-S100A8 antibody [EPR3554] (AB92331)
  • IHC

Lab

Immunohistochemistry - Anti-S100A8 antibody [EPR3554] (AB92331)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling S100A8 with ab92331 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101.

Positive staining on human tonsil.

The section was incubated with ab92331 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Western blot - Anti-S100A8 antibody [EPR3554] (AB92331)
  • WB

Lab

Western blot - Anti-S100A8 antibody [EPR3554] (AB92331)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-S100A8 antibody [EPR3554] (ab92331) at 1/1000 dilution

All lanes:

HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 11 kDa

false

Exposure time: 20s

Western blot - Anti-S100A8 antibody [EPR3554] (AB92331)
  • WB

Lab

Western blot - Anti-S100A8 antibody [EPR3554] (AB92331)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Expression of S100A8 is enhanced by stress response, can be simultaneously induced in monocytes, endothelial cells, keratinocytes or epithelial cells by several mediators. (PMID : 10779802, PMID 26661255).

All lanes:

Western blot - Anti-S100A8 antibody [EPR3554] (ab92331) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 20 µg

Lanes 2, 4 and 6:

Untreated THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 3:

THP-1 treated with 25ng/ml TNF alpha for 24 hours at 20 µg

Lane 5:

THP-1 treated with 100U/mL IFN gamma and 25ng/ml TNF alpha for 24 hours at 20 µg

Lane 7:

THP-1 treated with 100ng/ml LPS and 20ng/ml IFN gamma for 24 hours at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 11 kDa

false

Exposure time: 40s

Western blot - Anti-S100A8 antibody [EPR3554] (AB92331)
  • WB

Lab

Western blot - Anti-S100A8 antibody [EPR3554] (AB92331)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Expression levels of the target protein may vary with different batches of mouse spleen lysate.

All lanes:

Western blot - Anti-S100A8 antibody [EPR3554] (ab92331) at 1/1000 dilution

All lanes:

Mouse spleen lysate of different tissue batches at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 11 kDa

false

Exposure time: 180s

Western blot - Anti-S100A8 antibody [EPR3554] (AB92331)
  • WB

Lab

Western blot - Anti-S100A8 antibody [EPR3554] (AB92331)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of S100A8 under physiologic conditions is restricted to cells of myeloid origin including circulating neutrophils, monocytes, and eosinophils (PMID : 23241281, PMID : 1373330).

All lanes:

Western blot - Anti-S100A8 antibody [EPR3554] (ab92331) at 1/1000 dilution

Lane 1:

Mouse bone marrow tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse skin tissue lysate at 20 µg

Lane 4:

Mouse esophagus tissue lysate at 20 µg

Lane 5:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 11 kDa

false

Exposure time: 180s

Immunoprecipitation - Anti-S100A8 antibody [EPR3554] (AB92331)
  • IP

Unknown

Immunoprecipitation - Anti-S100A8 antibody [EPR3554] (AB92331)

ab92331 (purified) at 1/30 immunoprecipitating S100A8 in HL-60 (Human promyelocytic leukemia cell line) cell lysate (Lane 1).

Lane 2 - rabbit monoclonal IgG instead of ab92331 in HL-60 lysates. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1500).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-S100A8 antibody [EPR3554] (ab92331)

Predicted band size: 10 kDa

Observed band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3554

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p><strong>For unpurified use at 1/20.</strong></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/2000 - 1/10000.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250.</strong></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/2000 - 1/10000.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-S100A8 antibody [EPR3554] (ab92331) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of S100A8?
Anti-S100A8 [EPR3554] (ab92331) specifically detects a band for S100A8 (UniProt: P05109) at a molecular weight of 11kDa.

Trusted by the scientific community
Anti-S100A8 [EPR3554] (ab92331) was first used in a scientific publication in 2010 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-S100A8 [EPR3554] (ab92331) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR3554] also available for your convenience: ab92331, HRP - ab201473, Biotin - ab201474, Carrier free - ab219370, Carrier free - ab271863, Alexa Fluor® 647 - ab311157, Alexa Fluor® 594 - ab311805, Alexa Fluor® 568 - ab313088, Alexa Fluor® 555 - ab313282

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100A8 also known as calprotectin or MRP8 is a calcium-binding protein with a molecular weight of around 10.8 kDa. It is a member of the S100 protein family and is found expressed in myeloid cells such as neutrophils and monocytes. This protein often forms a heterodimer complex with S100A9 together referred to as calprotectin which plays a critical role in the immune response. Researchers frequently utilize calprotectin fecal ELISA MRP8 ELISA or calprotectin serum immunoassay to measure its levels in various biological samples.
Biological function summary

The S100A8/S100A9 complex modulates inflammatory processes and immune responses. It acts as a pro-inflammatory mediator and is associated with leukocyte recruitment to sites of inflammation. This complex plays an important role in protecting cells from infections by inhibiting bacterial growth through sequestration of nutrient metals showcasing antimicrobial properties. Calprotectin significantly impacts immune responses and has become an interesting target for calprotectin ELISA kits to quantify its presence in plasma and other bodily fluids indicating inflammation.

Pathways

S100A8 and its partner S100A9 are involved in toll-like receptor and receptor for advanced glycation end-products (RAGE) signaling pathways. These pathways are key in mediating inflammatory responses and linking to innate immunity. The calgranulin A ELISA is often used to evaluate their involvement in these pathways helping to elucidate interactions with other proteins like calmodulin that play a role in cellular regulation processes.

S100A8 is strongly associated with inflammatory conditions such as rheumatoid arthritis and inflammatory bowel disease. Its elevated expression levels serve as a biomarker for these conditions highlighting its potential for diagnostic use via calprotectin ELISA kits. S100A8's relationship with S100A9 supports its connection to these diseases reflecting the complex's significant presence and functional role in chronic inflammation and immune dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include : facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Participates also in regulatory T-cell differentiation together with CD69 (PubMed : 26296369). Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.. (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, may induce expansion of aberrant immature neutrophils in a TLR4-dependent manner.
See full target information S100A8
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Publications (70)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 12:1639277 PubMed41064510

2025

Identification of shared biomarkers and potential therapeutic targets for antiphospholipid syndrome and recurrent miscarriage by integrated bioinformatics analysis and machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Su Zhang,Yifang Zhang,Jing Xu,Weitao Hu,Xiaolan Huang,Xiaoqing Chen

Frontiers in immunology 16:1623486 PubMed40977740

2025

Myeloid-specific S100A8/A9 deficiency attenuates atrial fibrillation through prevention of TLR4/NF-kB-mediated immune cell recruitment and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Wang,Hua Shen,Jing Wang,Guifang Wang,Yufeng Zhang

Microbiology spectrum 13:e0044225 PubMed40704794

2025

Early immune response to is characterized by robust neutrophil and fibrotic macrophage recruitment and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia Miranda,Oscar A Davalos,Aimy Sebastian,Margarita V Rangel,Nicole F Leon,Bria M Gorman,Deepa K Murugesh,Nicholas R Hum,Gabriela G Loots,Katrina K Hoyer,Dina R Weilhammer

Inflammation : PubMed40608219

2025

A Comparative Analysis Dissecting the Immune Landscape of Vitiligo and Melanoma from a single-cell Perspective: Two Sides of the Same Coin?

Applications

Unspecified application

Species

Unspecified reactive species

Yongkai Yu,Yidan Wang,Jiawei Lu,Xuechen Cao,Yifei Feng,Tongxin Pei,Yan Lu

Frontiers in molecular biosciences 12:1530741 PubMed40270593

2025

S100A8/A9 high-expression macrophages mediate renal tubular epithelial cell damage in acute kidney injury following acute type A aortic dissection surgery.

Applications

Unspecified application

Species

Unspecified reactive species

Xiujuan Cai,Xin Li,Jian Shi,Lu Tang,Jie Yang,Ronghuang Yu,Zhigang Wang,Dongjin Wang

Cancer cell international 25:135 PubMed40200265

2025

Single-cell transcriptome sequencing reveals the mechanism of Realgar improvement on erythropoiesis in mice with myelodysplastic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Xu,Kexin Hu,Yanlu Wang,Shuyang Cai,Fan Wu,Jizhang Bao,Qi Hu,Yu Guan,Yuchen Tao,Jiahui Lu

PloS one 20:e0312587 PubMed39752388

2025

CD206+ Trem2+ macrophage accumulation in the murine knee joint after injury is associated with protection against post-traumatic osteoarthritis in MRL/MpJ mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jillian L McCool,Aimy Sebastian,Nicholas R Hum,Stephen P Wilson,Oscar A Davalos,Deepa K Murugesh,Beheshta Amiri,Cesar Morfin,Blaine A Christiansen,Gabriela G Loots

Frontiers in pharmacology 15:1460692 PubMed39386033

2024

Airspaces-derived exosomes contain disease-relevant protein signatures in a mouse model of cystic fibrosis (CF)-like mucoinflammatory lung disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Mao,Amol Suryawanshi,Sonika Patial,Yogesh Saini

Nature communications 15:7941 PubMed39266532

2024

Cellular origin and clonal evolution of human dedifferentiated liposarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nadège Gruel,Chloé Quignot,Laëtitia Lesage,Sophie El Zein,Sylvie Bonvalot,Dimitri Tzanis,Khadija Ait Rais,Fabien Quinquis,Bastien Manciot,Julien Vibert,Nadine El Tannir,Ahmed Dahmani,Héloïse Derrien,Didier Decaudin,Ivan Bièche,Laura Courtois,Odette Mariani,Laëtitia K Linares,Laurie Gayte,Sylvain Baulande,Joshua J Waterfall,Olivier Delattre,Gaëlle Pierron,Sarah Watson

International journal of molecular sciences 25: PubMed38791393

2024

A Pilot Study to Investigate Peripheral Low-Level Chronic LPS Injection as a Model of Neutrophil Activation in the Periphery and Brain in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle Aries,Makayla Cook,Tiffany Hensley-McBain
View all publications
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