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Rabbit Recombinant Monoclonal MRP8 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 52 publications.


Images

Immunoprecipitation - Anti-S100A8 antibody [EPR3554] (AB92331), expandable thumbnail
  • Western blot - Anti-S100A8 antibody [EPR3554] (AB92331), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (AB92331), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (AB92331), expandable thumbnail
  • Western blot - Anti-S100A8 antibody [EPR3554] (AB92331), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Tested
Not recommended
Expected

Tested
Tested

Species
Human
Dilution info
1/30
Notes

For unpurified use at 1/20.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Human
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

For unpurified use at 1/2000 - 1/10000.

Species
Human
Dilution info
1/1000
Notes

For unpurified use at 1/2000 - 1/10000.

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500 - 1/1000
Notes

For unpurified use at 1/100 - 1/250.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Participates also in regulatory T-cell differentiation together with CD69 (PubMed:26296369). Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity. (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, may induce expansion of aberrant immature neutrophils in a TLR4-dependent manner.

Alternative names

CAGA, CFAG, MRP8, S100A8, Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, Urinary stone protein band A, MRP-8, p8

Recommended products

Rabbit Recombinant Monoclonal MRP8 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 52 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3554
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

S100A8 also known as calprotectin or MRP8 is a calcium-binding protein with a molecular weight of around 10.8 kDa. It is a member of the S100 protein family and is found expressed in myeloid cells such as neutrophils and monocytes. This protein often forms a heterodimer complex with S100A9 together referred to as calprotectin which plays a critical role in the immune response. Researchers frequently utilize calprotectin fecal ELISA MRP8 ELISA or calprotectin serum immunoassay to measure its levels in various biological samples.

Biological function summary

The S100A8/S100A9 complex modulates inflammatory processes and immune responses. It acts as a pro-inflammatory mediator and is associated with leukocyte recruitment to sites of inflammation. This complex plays an important role in protecting cells from infections by inhibiting bacterial growth through sequestration of nutrient metals showcasing antimicrobial properties. Calprotectin significantly impacts immune responses and has become an interesting target for calprotectin ELISA kits to quantify its presence in plasma and other bodily fluids indicating inflammation.

Pathways

S100A8 and its partner S100A9 are involved in toll-like receptor and receptor for advanced glycation end-products (RAGE) signaling pathways. These pathways are key in mediating inflammatory responses and linking to innate immunity. The calgranulin A ELISA is often used to evaluate their involvement in these pathways helping to elucidate interactions with other proteins like calmodulin that play a role in cellular regulation processes.

Associated diseases and disorders

S100A8 is strongly associated with inflammatory conditions such as rheumatoid arthritis and inflammatory bowel disease. Its elevated expression levels serve as a biomarker for these conditions highlighting its potential for diagnostic use via calprotectin ELISA kits. S100A8's relationship with S100A9 supports its connection to these diseases reflecting the complex's significant presence and functional role in chronic inflammation and immune dysregulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-S100A8 antibody [EPR3554] (ab92331), expandable thumbnail

    Immunoprecipitation - Anti-S100A8 antibody [EPR3554] (ab92331)

    ab92331 (purified) at 1/30 immunoprecipitating S100A8 in HL-60 (Human promyelocytic leukemia cell line) cell lysate (Lane 1).

    Lane 2 - rabbit monoclonal IgG instead of ab92331 in HL-60 lysates. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1500).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-S100A8 antibody [EPR3554] (ab92331)

    Predicted band size: 10 kDa

    Observed band size: 11 kDa

  • Western blot - Anti-S100A8 antibody [EPR3554] (ab92331), expandable thumbnail

    Western blot - Anti-S100A8 antibody [EPR3554] (ab92331)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-S100A8 antibody [EPR3554] (ab92331) at 1/20000 dilution

    Lane 1: HL-60 (Human promyelocytic leukemia cell line) cell lysate at 20 µg

    Lane 2: Human fetal spleen tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 10 kDa

    Observed band size: 11 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (ab92331), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (ab92331)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labeling S100A8 with purified ab92331 at 1/500.

    Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0. Goat Anti-Rabbit IgG H&L (HRP) ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

    Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (ab92331), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (ab92331)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labeling S100A8 with unpurified ab92331.

    Detection: DAB staining.

    Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • Western blot - Anti-S100A8 antibody [EPR3554] (ab92331), expandable thumbnail

    Western blot - Anti-S100A8 antibody [EPR3554] (ab92331)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-S100A8 antibody [EPR3554] (ab92331) at 1/2000 dilution

    All lanes: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 10 kDa

    Observed band size: 11 kDa

  • Western blot - Anti-S100A8 antibody [EPR3554] (ab92331), expandable thumbnail

    Western blot - Anti-S100A8 antibody [EPR3554] (ab92331)

    All lanes: Western blot - Anti-S100A8 antibody [EPR3554] (ab92331)

    Lane 1: Human spleen tissue lysate at 10 µg

    Lane 2: PBMC cell lysate at 10 µg

    Lane 3: HL-60 (Human promyelocytic leukemia cell line) cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 10 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (ab92331), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (ab92331)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling S100A8 with unpurified ab92331. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (ab92331), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (ab92331)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labeling S100A8 with unpurified ab92331.

    Detection: DAB staining.

    Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (ab92331), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 antibody [EPR3554] (ab92331)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling S100A8 with unpurified ab92331. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

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Product protocols

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