Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free
- BOND RX™ Validated
- RabMAb
- Recombinant
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Rabbit Recombinant Multiclonal MRP8 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), WB, IHC-P, IHC-Fr, ICC/IF and reacts with Mouse, Human, Rat samples.
View Alternative Names
CAGA, CFAG, MRP8, S100A8, Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, Urinary stone protein band A, MRP-8, p8
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free (AB288578)
This data was developed using ab288715, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized HL-60 (Human acute promyelocytic leukemia promyeloblast) cells labelling S100A8+S100A9 with ab288715 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free (AB288578)
This data was developed using ab288715, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling S100A8+S100A9 with ab288715 at 1/5000 (0.096 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on the human spleen. The section was incubated with ab288715 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free (AB288578)
This data was developed using ab288715, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HL-60 cells labelling S100A8+S100A9 with ab288715 at 1/500 (0.96 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in HL-60 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free (AB288578)
This data was developed using ab288715, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human stomach carcin tissue labeling S100A8+S100A9 with ab288715 at 1/5000 (0.096 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on the stroma inflammatory cells in human stomach carcinoma. The section was incubated with ab288715 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
- IP
Lab
Immunoprecipitation - Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free (AB288578)
This data was developed using ab288715, the same antibody clone in a different buffer formulation.
S100A8+S100A9 was immunoprecipitated from 0.35 mg HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate 10ug with ab288715 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288715 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate 10ug
Lane 2 : ab288715 IP in HL-60 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab288715 in HL-60 whole cell lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds
All lanes:
Immunoprecipitation - Anti-S100A8 + S100A9 antibody [RM1038] (<a href='/en-us/products/primary-antibodies/s100a8-s100a9-antibody-rm1038-ab288715'>ab288715</a>)
Predicted band size: 13 kDa
Observed band size: 11 kDa,14 kDa
false
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free (AB288578)
This data was developed using ab288715, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse blood cells cells labelling S100A8+S100A9 with ab288715 at 1/500 dilution (0.1 μg)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free (AB288578)
This data was developed using ab288715, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling S100A8+S100A9 with ab288715 at 1/5000 (0.096 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the rat spleen. The section was incubated with ab288715 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free (AB288578)
This data was developed using ab288715, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling S100A8+S100A9 with ab288715 at 1/5000 (0.096 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the mouse spleen. The section was incubated with ab288715 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free (AB288578)
This data was developed using ab288715, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC cells labelling S100A8+S100A9 with ab288715 at 1/500 (0.96 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in subsets of mouse PBMCs is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
- IP
Lab
Immunoprecipitation - Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free (AB288578)
This data was developed using ab288715, the same antibody clone in a different buffer formulation.
S100A8+S100A9 was immunoprecipitated from 0.35 mg Mouse spleen lysate 10ug with ab288715 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288715 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1 : Mouse spleen lysate 10ug
Lane 2 : ab288715 IP in Mouse spleen lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab288715 in Mouse spleen lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds
All lanes:
Immunoprecipitation - Anti-S100A8 + S100A9 antibody [RM1038] (<a href='/en-us/products/primary-antibodies/s100a8-s100a9-antibody-rm1038-ab288715'>ab288715</a>)
Predicted band size: 13 kDa
Observed band size: 11 kDa,14 kDa
false
- WB
Lab
Western blot - Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free (AB288578)
This data was developed using ab288715, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Exposure time : 3 min
All lanes:
Western blot - Anti-S100A8 + S100A9 antibody [RM1038] (<a href='/en-us/products/primary-antibodies/s100a8-s100a9-antibody-rm1038-ab288715'>ab288715</a>) at 1/1000 dilution
Lane 1:
Mouse spleen lysate
Lane 2:
Rat spleen lysate
Lane 3:
SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4:
HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 13 kDa
Observed band size: 11 kDa,14 kDa
false
- WB
Lab
Western blot - Anti-S100A8 + S100A9 antibody [RM1038] - BSA and Azide free (AB288578)
This data was developed using ab288715, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Exposure time : 3 min
All lanes:
Western blot - Anti-S100A8 + S100A9 antibody [RM1038] (<a href='/en-us/products/primary-antibodies/s100a8-s100a9-antibody-rm1038-ab288715'>ab288715</a>) at 1/1000 dilution
Lane 1:
Mouse PBMC lysate
Lane 2:
Mouse spleen lysate
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 13 kDa
Observed band size: 11 kDa,14 kDa
false
Related conjugates and formulations (1)
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Anti-S100A8 + S100A9 antibody [RM1038]
Reactivity data
Product details
ab288578 is the carrier free version of ab288715.
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Calprotectin serves as an important player in the immune response. This complex possesses antimicrobial properties and contributes to the body's defense by modulating inflammation. It binds to zinc and manganese to deprive pathogens of essential nutrients an action called nutrient bioavailability restriction. In immune cells calprotectin's presence signals the activation and recruitment of further immune components.
Pathways
S100A8 and S100A9 influence the NF-kB signaling pathway which governs immune responses and inflammation. Their function affects other pathways such as MAPK by mediating pro-inflammatory cytokines' production and release. These proteins often engage with Toll-like receptors impacting the innate immune system and contributing to the body's defensive mechanisms against pathogens.
Product protocols
- Visit the General protocols
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Target data
Additional targets
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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