Skip to main content

Goat Polyclonal S100A9 antibody. Suitable for ICC, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human S100A9 aa 50-100.

Be the first to review this product! Submit a review

Images

Immunocytochemistry - Anti-S100A9 antibody (AB81522), expandable thumbnail
  • Immunocytochemistry - Anti-S100A9 antibody (AB81522), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-S100A9 antibody (AB81522), expandable thumbnail
  • Western blot - Anti-S100A9 antibody (AB81522), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody (AB81522), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Goat

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human S100A9 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P06702

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Cow
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

-

Predicted
Predicted

Species

Rabbit, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.5-2 µg/mL

Notes

1 hour primary incubation is recommended for this product.

Predicted
Predicted

Species

Rabbit, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

2.5 µg/mL

Notes

-

Predicted
Predicted

Species

Rabbit, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rabbit, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response (PubMed:12626582, PubMed:15331440, PubMed:16258195, PubMed:19122197, PubMed:20103766, PubMed:21325622, PubMed:8423249). It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism (PubMed:12626582, PubMed:15331440, PubMed:20103766). Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions (PubMed:16258195, PubMed:19122197, PubMed:8423249). The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase (PubMed:15331440, PubMed:21325622). Participates also in regulatory T-cell differentiation together with CD69 (PubMed:26296369). Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX (PubMed:15642721, PubMed:22808130). The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities (PubMed:19534726, PubMed:8423249). Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration (PubMed:15598812, PubMed:21487906). Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER) (PubMed:19402754). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade (PubMed:19402754, PubMed:22804476). Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth (PubMed:19087201). Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3 (PubMed:19935772). Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK (PubMed:22363402). Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants (PubMed:21912088, PubMed:22489132). Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread (PubMed:16258195). Has transnitrosylase activity; in oxidatively-modified low-densitity lipoprotein (LDL(ox))-induced S-nitrosylation of GAPDH on 'Cys-247' proposed to transfer the NO moiety from NOS2/iNOS to GAPDH via its own S-nitrosylated Cys-3 (PubMed:25417112). The iNOS-S100A8/A9 transnitrosylase complex is proposed to also direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif (PubMed:25417112).

Alternative names

Recommended products

Goat Polyclonal S100A9 antibody. Suitable for ICC, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human S100A9 aa 50-100.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human S100A9 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P06702
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

S100A9 also known as calgranulin B or MRP-14 is a calcium-binding protein with a molecular weight of approximately 13 kDa. It belongs to the S100 family and forms part of a heterodimeric complex with S100A8. S100A9 is largely found in myeloid cells such as neutrophils and monocytes. It plays a significant role in the cytosol and can be secreted to the extracellular space under specific stress conditions.

Biological function summary

S100A9 impacts inflammatory responses and immune regulation. It partners with S100A8 to form the calprotectin complex which acts as a strong pro-inflammatory mediator. This complex binds to receptors like RAGE and TLR4 initiating signaling pathways that promote inflammation. S100A9 also participates in leukocyte recruitment and has antimicrobial properties blocking the growth of bacteria and fungi by chelating essential metal ions.

Pathways

Several are influenced by S100A9 including the NF-kB and MAPK signaling pathways. Through its interaction with RAGE and TLR4 receptors S100A9 influences the activation of these pathways which are important in the regulation of inflammatory and immune responses. Other proteins involved in these pathways include MyD88 for TLR4 signaling and various kinases for MAPK signaling positioning S100A9 as an important regulator within the inflammatory networks.

Associated diseases and disorders

S100A9 links to various inflammatory and autoimmune conditions such as rheumatoid arthritis and inflammatory bowel disease. Increased levels of S100A9 have been observed in these conditions where it augments the inflammatory response. In rheumatoid arthritis for example S100A9 upregulation correlates with increased joint inflammation and damage. The protein’s interaction with S100A8 in these diseases highlights its contribution to the pathogenesis and progression of inflammation-related conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry - Anti-S100A9 antibody (ab81522), expandable thumbnail

    Immunocytochemistry - Anti-S100A9 antibody (ab81522)

    Immunocyohemistry analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mL, showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10ug/mL, followed by Alexa Fluor 488 secondary antibody at 2ug/mL.

  • Immunocytochemistry - Anti-S100A9 antibody (ab81522), expandable thumbnail

    Immunocytochemistry - Anti-S100A9 antibody (ab81522)

    Immunocytochemistry analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mL, showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mlL.

  • Flow Cytometry (Intracellular) - Anti-S100A9 antibody (ab81522), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-S100A9 antibody (ab81522)

    Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr at 10ug/mL, followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

  • Western blot - Anti-S100A9 antibody (ab81522), expandable thumbnail

    Western blot - Anti-S100A9 antibody (ab81522)

    All lanes: Western blot - Anti-S100A9 antibody (ab81522) at 1 µg/mL

    Lane 1: Human bone marrow

    Lane 2: Gastrointestinal cancer lysate

    Lane 3: negative control HepG2 lysate.

    Predicted band size: 13 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody (ab81522), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody (ab81522)

    Immunohistochemical analysis of paraffin embedded human lung tissue labelling S100A9 with ab81522 at 2.5μg/ml.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com