Anti-S100P antibody [EPR6142]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (AB124743)

Unpurified ab124743 showing positive staining in colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (AB124743)

Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human placenta tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (AB124743)

Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human pancreatic adenocarcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (AB124743)

Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human spleen tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-S100P antibody [EPR6142] (AB124743)

Immunofluorescence staining of BxPC-3 cells with purified ab124743 at a working dilution of 1 in 150, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 555 goat anti-rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab124743 was used at a dilution of 1/200 followed by an Alexa Fluor^® 555 goat anti-mouse antibody at a dilution of 1/500.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (AB124743)

Immunohistochemical staining of paraffin embedded human pancreatic adenocarcinoma with purified ab124743 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-S100P antibody [EPR6142] (AB124743)

Intracellular Flow Cytometry analysis of BxPC-3 (human Pancreas adenocarcinoma) cells labeling S100P with purified ab124743 at 1/1700 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

• WB

Lab

Western blot - Anti-S100P antibody [EPR6142] (AB124743)

ab124743 was shown to react with S100P in wild-type HeLa cells in western blot. Loss of signal was observed when S100P knockout sample was used. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab124743 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.

All lanes:

Western blot - Anti-S100P antibody [EPR6142] (ab124743)

Predicted band size: 10 kDa

Observed band size: 9 kDa

false

Exposure time: 20min

• WB

Unknown

Western blot - Anti-S100P antibody [EPR6142] (AB124743)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-S100P antibody [EPR6142] (ab124743) at 1/1000 dilution

All lanes:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 10 kDa

Observed band size: 10 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (AB124743)

Unpurified ab124743 showing negative staining in normal liver tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (AB124743)

Unpurified ab124743 showing negative staining in normal pancreas tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-S100P antibody [EPR6142] (AB124743)

All lanes:

Western blot - Anti-S100P antibody [EPR6142] (ab124743) at 1/1000 dilution

All lanes:

BxPC3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 10 kDa

Observed band size: 10 kDa

false