Name: Anti-S100P antibody [EPR6143]
SKU: ab133554
Rating: 3 (1 reviews)

Rabbit Recombinant Monoclonal S100P antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 13 publications.

Related conjugates and formulations

ab225543
Carrier free
Anti-S100P antibody [EPR6143] - BSA and Azide free
ab306248
Conjugated
APC Anti-S100P antibody [EPR6143]
ab306249
Conjugated
HRP Anti-S100P antibody [EPR6143]
ab310488
Conjugated
Alexa Fluor® 594 Anti-S100P antibody [EPR6143]
ab310085
Conjugated
Alexa Fluor® 647 Anti-S100P antibody [EPR6143]
ab312494
Conjugated
Alexa Fluor® 568 Anti-S100P antibody [EPR6143]
ab312017
Conjugated
Alexa Fluor® 555 Anti-S100P antibody [EPR6143]
ab306247
Conjugated
PE Anti-S100P antibody [EPR6143]
ab309719
Conjugated
Alexa Fluor® 488 Anti-S100P antibody [EPR6143]
ab321052
Conjugated
Alexa Fluor® 750 Anti-S100P antibody [EPR6143]

Images

Western blot - Anti-S100P antibody [EPR6143] (AB133554), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1500	Notes For unpurified use at 1/150 - 1/100. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/20	Notes For unpurified use at 1/80.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes For unpurified use at 1/200.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/400	Notes For unpurified use at 1/40.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target data

See full target information S100P

Function

May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE).

Alternative names

S100E, S100P, Protein S100-P, Migration-inducing gene 9 protein, Protein S100-E, S100 calcium-binding protein P, MIG9

Recommended products

Rabbit Recombinant Monoclonal S100P antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 13 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR6143
Purification technique: Affinity purification Protein A
Dissociation constant: 3.16 x 10^-10 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

S100P also known as Protein S100-P is a member of the S100 family of proteins containing 94 amino acids with a mass of approximately 10 kDa. Its structure includes two EF-hand calcium-binding motifs which allow it to bind calcium ions effectively. You can find S100P in various tissues but it is highly expressed in the placenta prostate lungs and kidneys. These expression patterns suggest it has multiple roles in different cellular contexts.

Biological function summary

S100P plays a role in intracellular and extracellular calcium signaling. It influences cell survival proliferation and migration. It can function as a monomer but often interacts with other proteins forming complexes such as with RAGE (Receptor for Advanced Glycation End-products) which amplifies signaling cascades that control cellular responses. Its binding to calcium and interaction with RAGE modulate important cellular processes tied to cell cycle and cellular stress response.

Pathways

S100P is closely involved in the MAPK (Mitogen-Activated Protein Kinases) and RAGE signaling pathways. Its interaction with RAGE leads to activation of the MAPK pathway facilitating cellular responses such as growth and survival. S100P also coordinates with other S100 proteins such as S100A4 and S100A9 enhancing its involvement in regulating cellular functions through these pathways. These interactions highlight the importance of S100P in cellular communication and response mechanisms.

Associated diseases and disorders

S100P is associated with various cancers like pancreatic and breast cancer. Its expression level often increases in malignancies where it promotes metastasis and poor prognosis. Through the RAGE pathway S100P influences tumor progression by altering cellular adhesion migration and immune response. In cancer biology it links with proteins such as E-cadherin and matrix metalloproteinases which further contribute to tumor invasiveness and metastasis making it a potential target for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Western blot - Anti-S100P antibody [EPR6143] (ab133554)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-S100P antibody [EPR6143] (ab133554) at 1/200 dilution
Lane 1: SW480 cell lysate at 10 µg
Lane 2: BxPC-3 cell lysate at 10 µg
Secondary
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6143] (ab133554)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling S100P with unpurified ab133554 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-S100P antibody [EPR6143] (ab133554)
Immunocytochemistry/Immunofluorescence analysis of SW480 cells labelling S100P (green) with pruified ab133554 at 1/40. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6143] (ab133554)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling S100P with purified ab133554 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6143] (ab133554)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic adenocarcinoma tissue labelling S100P with unpurified ab133554 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-S100P antibody [EPR6143] (ab133554)
Intracellular Flow Cytometry analysis of BxPC-3 (human Pancreas adenocarcinoma) cells labeling S100P with purified ab133554 at 1/800 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunoprecipitation - Anti-S100P antibody [EPR6143] (ab133554)
ab133554 (unpurified) at 1/20 immunoprecipitating S100P in SW480 cells. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-S100P antibody [EPR6143] (ab133554)
Predicted band size: 10 kDa
Observed band size: 10 kDa
Western blot - Anti-S100P antibody [EPR6143] (ab133554)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-S100P antibody [EPR6143] (ab133554) at 1/1500 dilution
Lane 1: SW480 cell lysate at 10 µg
Lane 2: BxPC-3 cell lysate at 10 µg
Secondary
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDa
Immunoprecipitation - Anti-S100P antibody [EPR6143] (ab133554)
ab133554 (purified) at 1/80 immunoprecipitating S100P in SW480 cells. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-S100P antibody [EPR6143] (ab133554)
Predicted band size: 10 kDa
Observed band size: 10 kDa
Western blot - Anti-S100P antibody [EPR6143] (ab133554)
All lanes: Western blot - Anti-S100P antibody [EPR6143] (ab133554) at 1/1000 dilution
Lane 1: SW480 cell lysate at 10 µg
Lane 2: Human placenta cell lysate at 10 µg
Lane 3: BxPC-3 cell lysate at 10 µg
Secondary
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDa
Immunocytochemistry/ Immunofluorescence - Anti-S100P antibody [EPR6143] (ab133554)
Immunocytochemistry/Immunofluorescence analysis of SW480 cells labelling S100P (green) with pruified ab133554 at 1/400. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
OI-RD Scanning - Anti-S100P antibody [EPR6143] (ab133554)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.