Rabbit Polyclonal S1P antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 6 publications.
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- Brain research 1799:1481712022Dysregulation of sphingosine-1-phosphate (S1P) and S1P receptor 1 signaling in the 5xFAD mouse model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYounghun Jung,Jonathan Lopez-Benitez,Christina M Tognoni,Isabel Carreras,Alpaslan DedeogluPubMed 36410428
- The Journal of biological chemistry 298:1019362022AAA-ATPase valosin-containing protein binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKoji Shibuya,Ken Ebihara,Chihiro Ebihara,Nagisa Sawayama,Masayo Isoda,Daisuke Yamamuro,Manabu Takahashi,Shuichi Nagashima,Shun IshibashiPubMed 35430252
- Food science & nutrition 9:209-2162021Curcumin inhibits the proteolytic process of SREBP-2 by first inhibiting the expression of S1P rather than directly inhibiting SREBP-2 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongnan Li,Shuodong WuPubMed 33473285
- The Journal of biological chemistry 295:17713-177232021Hdac3 regulates bone modeling by suppressing osteoclast responsiveness to RANKL.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid H H Molstad,Anna M Mattson,Dana L Begun,Jennifer J Westendorf,Elizabeth W BradleyPubMed 33454009
- Immunity 49:842-856.e72018Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuansheng Guo,Zhexu Chi,Danlu Jiang,Ting Xu,Weiwei Yu,Zhen Wang,Sheng Chen,Li Zhang,Qianyun Liu,Xingchen Guo,Xue Zhang,Wenxin Li,Linrong Lu,Yingliang Wu,Bao-Liang Song,Di WangPubMed 30366764
- Cell 175:133-145.e152018ER Stress Drives Lipogenesis and Steatohepatitis via Caspase-2 Activation of S1P.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJu Youn Kim et. al.PubMed 30220454
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Tested |
| Rat | Tested |
| Chicken | Predicted |
| Chimpanzee | Predicted |
| Chinese hamster | Predicted |
| Gorilla | Predicted |
| Horse | Predicted |
| Macaque monkey | Predicted |
| Orangutan | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Chicken, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Serine protease that cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4: known substrates include SREBF1/SREBP1, SREBF2/SREBP2, BDNF, GNPTAB, ATF6, ATF6B and FAM20C (PubMed:10644685, PubMed:12782636, PubMed:21719679, PubMed:34349020). Cleaves substrates after Arg-Ser-Val-Leu (SREBP2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu (PubMed:10644685, PubMed:21719679). Catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed:12782636). Also mediates the first step in the proteolytic activation of the cyclic AMP-dependent transcription factor ATF-6 (ATF6 and ATF6B) (PubMed:12782636). Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes (PubMed:21719679). Cleaves the propeptide from FAM20C which is required for FAM20C secretion from the Golgi apparatus membrane and for enhancement of FAM20C kinase activity, promoting osteoblast differentiation and biomineralization (PubMed:34349020). Involved in the regulation of M6P-dependent Golgi-to-lysosome trafficking of lysosomal enzymes (PubMed:21719679, PubMed:30046013). It is required for the activation of CREB3L2/BBF2H7, a transcriptional activator of MIA3/TANGO and other genes controlling mega vesicle formation (PubMed:30046013). Therefore, it plays a key role in the regulation of mega vesicle-mediated collagen trafficking (PubMed:30046013). In astrocytes and osteoblasts, upon DNA damage and ER stress, mediates the first step of the regulated intramembrane proteolytic activation of the transcription factor CREB3L1, leading to the inhibition of cell-cycle progression (PubMed:16417584).
Alternative names
KIAA0091, S1P, SKI1, MBTPS1, Membrane-bound transcription factor site-1 protease, Endopeptidase S1P, Subtilisin/kexin-isozyme 1, SKI-1
Recommended products
Rabbit Polyclonal S1P antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sphingosine-1-phosphate (S1P) also known as SPC is a signaling sphingolipid with a molecular mass of approximately 379 Da. It plays an important role in cellular signaling by binding to a family of G-protein-coupled receptors known as S1P receptors (S1PR1-5). S1P is expressed in various tissues including the immune system cardiovascular system and central nervous system. It exhibits a strong influence on cell survival proliferation and migration through its interactions with these receptors.
- Biological function summary
S1P regulates immune cell trafficking vascular maturation and endothelial cell integrity. It acts in concert with other bioactive lipids as part of larger signaling complexes. This signaling molecule influences the barrier function of endothelial cells and it exerts control over lymphocyte egress from secondary lymphoid organs. S1P is also implicated in the stabilization of endothelial adherens junctions further highlighting its role in maintaining vascular homeostasis.
- Pathways
The S1P signaling contributes significantly to the sphingolipid metabolism pathway and the MAPK signaling pathway. In the sphingolipid pathway S1P is synthesized from sphingosine by sphingosine kinases and subsequently acts through its receptors to exert various cellular effects. The MAPK signaling pathway involves proteins like ERK where S1P can influence cellular responses like proliferation and survival. This tight regulation of signaling pathways secures S1P's role as a central lipid mediator in processes such as inflammation and angiogenesis.
- Associated diseases and disorders
S1P has associations with multiple sclerosis and cancer. In multiple sclerosis S1P modulation affects lymphocyte circulation with its receptor S1PR1 being an important player in the disease's treatment approach by controlling lymphocyte migration. In cancer S1P signaling can promote tumor growth and metastasis linked to proteins like VEGF which enhance angiogenesis. Understanding S1P's complex interactions in these diseases enables the development of therapeutic targets to mitigate pathological conditions.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-S1P antibody (ab140592)
The band observed at 100 kDa could potentially be a cleaved form of S1P due to the presence of a 17 amino acid signal peptide and a 169 amino acid pro-peptide.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140592 overnight at 4°C.
All lanes: Western blot - Anti-S1P antibody (ab140592) at 1 µg/mL
Lane 1: Mouse liver tissue lysate at 10 µg
Lane 2: Rat liver tissue lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 100 kDa, 62 kDa
Exposure time: 90s
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