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AB224618

Anti-S1P antibody - C-terminal

4

(3 Reviews)

|

(3 Publications)

Rabbit Polyclonal S1P antibody. C-terminal. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MBTPS1 aa 800 to C-terminus.

View Alternative Names

KIAA0091, S1P, SKI1, MBTPS1, Membrane-bound transcription factor site-1 protease, Endopeptidase S1P, Subtilisin/kexin-isozyme 1, SKI-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P antibody - C-terminal (AB224618)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P antibody - C-terminal (AB224618)

Paraffin-embedded human small intestine tissue stained for S1P using ab224618 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P antibody - C-terminal (AB224618)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P antibody - C-terminal (AB224618)

Paraffin-embedded human lung tissue stained for S1P using ab224618 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MBTPS1 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14703

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sphingosine-1-phosphate (S1P) also known as SPC is a signaling sphingolipid with a molecular mass of approximately 379 Da. It plays an important role in cellular signaling by binding to a family of G-protein-coupled receptors known as S1P receptors (S1PR1-5). S1P is expressed in various tissues including the immune system cardiovascular system and central nervous system. It exhibits a strong influence on cell survival proliferation and migration through its interactions with these receptors.
Biological function summary

S1P regulates immune cell trafficking vascular maturation and endothelial cell integrity. It acts in concert with other bioactive lipids as part of larger signaling complexes. This signaling molecule influences the barrier function of endothelial cells and it exerts control over lymphocyte egress from secondary lymphoid organs. S1P is also implicated in the stabilization of endothelial adherens junctions further highlighting its role in maintaining vascular homeostasis.

Pathways

The S1P signaling contributes significantly to the sphingolipid metabolism pathway and the MAPK signaling pathway. In the sphingolipid pathway S1P is synthesized from sphingosine by sphingosine kinases and subsequently acts through its receptors to exert various cellular effects. The MAPK signaling pathway involves proteins like ERK where S1P can influence cellular responses like proliferation and survival. This tight regulation of signaling pathways secures S1P's role as a central lipid mediator in processes such as inflammation and angiogenesis.

S1P has associations with multiple sclerosis and cancer. In multiple sclerosis S1P modulation affects lymphocyte circulation with its receptor S1PR1 being an important player in the disease's treatment approach by controlling lymphocyte migration. In cancer S1P signaling can promote tumor growth and metastasis linked to proteins like VEGF which enhance angiogenesis. Understanding S1P's complex interactions in these diseases enables the development of therapeutic targets to mitigate pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine protease that cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4 : known substrates include SREBF1/SREBP1, SREBF2/SREBP2, BDNF, GNPTAB, ATF6, ATF6B and FAM20C (PubMed : 10644685, PubMed : 12782636, PubMed : 21719679, PubMed : 34349020). Cleaves substrates after Arg-Ser-Val-Leu (SREBP2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu (PubMed : 10644685, PubMed : 21719679). Catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed : 12782636). Also mediates the first step in the proteolytic activation of the cyclic AMP-dependent transcription factor ATF-6 (ATF6 and ATF6B) (PubMed : 12782636). Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes (PubMed : 21719679). Cleaves the propeptide from FAM20C which is required for FAM20C secretion from the Golgi apparatus membrane and for enhancement of FAM20C kinase activity, promoting osteoblast differentiation and biomineralization (PubMed : 34349020). Involved in the regulation of M6P-dependent Golgi-to-lysosome trafficking of lysosomal enzymes (PubMed : 21719679, PubMed : 30046013). It is required for the activation of CREB3L2/BBF2H7, a transcriptional activator of MIA3/TANGO and other genes controlling mega vesicle formation (PubMed : 30046013). Therefore, it plays a key role in the regulation of mega vesicle-mediated collagen trafficking (PubMed : 30046013). Promotes FAD binding to the ETFA/ETFB complex and is therefore involved in mitochondrial respiratory chain regulation (PubMed : 35362222). In astrocytes and osteoblasts, upon DNA damage and ER stress, mediates the first step of the regulated intramembrane proteolytic activation of the transcription factor CREB3L1, leading to the inhibition of cell-cycle progression (PubMed : 16417584).
See full target information MBTPS1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 23:e14303 PubMed39113346

2024

The activation of cGAS-STING pathway causes abnormal uterine receptivity in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Ting Chen,Wen-Wen Shi,Feng Ran,Cheng-Kan Liu,Hui-Na Luo,Li-Juan Wu,Ying Wu,Tong-Tong Zhang,Zeng-Ming Yang

Laboratory animal research 39:27 PubMed37941082

2023

Feline mammary carcinoma-derived extracellular vesicle promotes liver metastasis via sphingosine kinase-1-mediated premetastatic niche formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chih Chang,Hao-Ping Liu,Hsiao-Li Chuang,Jiunn-Wang Liao,Pei-Ling Kao,Hsun-Lung Chan,Ter-Hsin Chen,Yu-Chih Wang

Computational and mathematical methods in medicine 2022:5985375 PubMed35872958

2022

Inhibition of SphK1/S1P Signaling Pathway Alleviates Fibrosis and Inflammation of Rat Myocardium after Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaokui Wu,Junwei Xu,Xiangyu Li,Jian Dai,Linlin Wang
View all publications
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Product promise

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