JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB11424

Anti-S1P1/EDG1 antibody

2

(3 Reviews)

|

(41 Publications)

Rabbit Polyclonal S1P1/EDG1 antibody. Suitable for ICC, IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human, Transfected cell line samples. Cited in 41 publications. Immunogen corresponding to Synthetic Peptide within Mouse S1pr1 aa 350 to C-terminus.

View Alternative Names

CD363, CHEDG1, EDG1, S1PR1, Sphingosine 1-phosphate receptor 1, S1P receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, Sphingosine 1-phosphate receptor Edg-1, S1P receptor Edg-1

2 Images
Immunocytochemistry - Anti-S1P1/EDG1 antibody (AB11424)
  • ICC

Supplier Data

Immunocytochemistry - Anti-S1P1/EDG1 antibody (AB11424)

Immunocytochemical analysis of Neuro-2a cells, labeling S1P1/EDG1 with ab11424. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Immunostaining with anti-EDG1 diluted 2μg/ml in 0.1% BSA for 3 hours at room temperature.

Green staining - ab11424

Blue staining - DAPI for nuclear localization

Red staining - F-actin

The right-most image is a control (no primary antibody staining).

Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody (AB11424)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody (AB11424)

ab11424 staining S1P1/EDG1 in transfected CCL39 cells by Immunocytochemistry/ Immunofluorescence.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC, Flow Cyt, IP, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Mouse S1pr1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O08530

Specificity

ab11424 detects S1P1/EDG1 protein in transfected human cell samples. This antibody shows no cross-reactivity to S1P3.

style
left-column

Alternative Products

Primary Antibodies

AB233386

Anti-S1P1/EDG1 antibody [EPR21202]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody [EPR21202] (AB233386)

  • 0
  • 0
Primary Antibodies

AB111571

Anti-S1P1/EDG1 (phospho T236) antibody

Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 (phospho T236) antibody (AB111571)

  • 0
  • 1
Primary Antibodies

AB77076

Anti-S1P1/EDG1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody (AB77076)

  • 4
  • 3
Primary Antibodies

AB242085

Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody [EPR21202] - BSA and Azide free (AB242085)

  • 0
  • 0
Primary Antibodies

AB140952

Anti-S1P1/EDG1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody (AB140952)

  • 4
  • 2

Complementary Products

Primary Antibodies

AB235919

Anti-S1PR2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-S1PR2 antibody (AB235919)

  • 1
  • 1
Primary Antibodies

AB224618

Anti-S1P antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P antibody - C-terminal (AB224618)

  • 4
  • 3
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB64693

Anti-CD206 antibody

BOND RX™ Validated

Western blot - Anti-CD206 antibody (AB64693)

  • 5
  • 42
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2 µg/mL", "ICC-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>Band represents recombinant S1P1 from transfected HEK293 cells.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S1P1 receptor also known as EDG1 is a G-protein-coupled receptor that plays a significant role in the sphingosine-1-phosphate (S1P) signaling pathway. With a molecular mass of approximately 43 kDa S1P1 is expressed in various tissues such as vascular endothelial cells immune cells and cardiac cells. The protein mediates the effects of sphingosine-1-phosphate a bioactive lipid that participates in various physiological processes.
Biological function summary

S1P1 is involved in the regulation of immune cell trafficking and vascular stability. As a part of a receptor complex it activates intracellular signaling cascades that influence cell migration and vascular maturation. By binding to S1P S1P1 controls the egress of lymphocytes from lymphoid tissues indicating its important role in immune surveillance and response.

Pathways

S1P1 serves as an important component in the S1P signaling pathway and the phosphoinositide 3-kinase (PI3K) pathway. In the S1P pathway it regulates endothelial cell barrier function and vascular maturation. In the PI3K pathway S1P1 influences cell survival and migration which relates it to protein kinases like Akt and other signaling molecules that further modify cellular responses.

S1P1 is implicated in multiple sclerosis (MS) and cancer progression. In MS S1P1 modulation affects lymphocyte trafficking which is the mechanism behind the action of fingolimod (FTY720) a therapeutic agent for the disease. S1P1 also connects to VEGF and other angiogenic factors in cancer where it influences tumor angiogenesis and metastasis. Understanding these connections highlights the potential for targeting S1P1 in therapeutic strategies for these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis (By similarity). Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury.
See full target information S1PR1
style
left-column

Publications (41)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:31 PubMed39833165

2025

S1PR3-driven positive feedback loop sustains STAT3 activation and keratinocyte hyperproliferation in psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Panpan Lian,Li Li,Renwei Lu,Bin Zhang,Junaid Wazir,Chaode Gu,Bojie Ma,Wenyuan Pu,Wangsen Cao,Zhiqiang Huang,Zhonglan Su,Hongwei Wang

iScience 27:111290 PubMed39618500

2024

Broad and diverse roles of sphingosine-1-phosphate/sphingosine-1-phosphate receptors in the prostate.

Applications

Unspecified application

Species

Unspecified reactive species

Daoquan Liu,Jianmin Liu,Yan Li,Lu Du,Qingqiong Cao,Liang Yang,Yongying Zhou,Ping Chen,Yuming Guo,Guang Zeng,Michael E DiSanto,Weidong Hu,Xinhua Zhang

Cells 12: PubMed37830557

2023

Presynaptic Release-Regulating Sphingosine 1-Phosphate 1/3 Receptors in Cortical Glutamatergic Terminals: Adaptations in EAE Mice and Impact of Therapeutic FTY720.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra Roggeri,Guendalina Olivero,Cesare Usai,Tim Vanmierlo,Anna Pittaluga

Advanced healthcare materials 12:e2301407 PubMed37591196

2023

Sphingosine-1-Phosphate Receptor Targeted PLGA Nanobubbles for Inflammatory Vascular Endothelial Cell Catching.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Tang,Yang Liu,Mingxi Li,Xiao Wang,Anning Du,Ning Gu,Fang Yang

Heliyon 9:e19109 PubMed37636348

2023

Xiaoyin Jiedu Granules may alleviate psoriasis-like skin diseases in mice by regulating sphingosine 1-phosphate receptor expression and reducing Th17 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zi Wang,Guangzhong Zhang,Haomin Zhang,Lingling Li

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23061 PubMed37389926

2023

Sphingosine 1-phosphate elicits a ROS-mediated proinflammatory response in human endometrial stromal cells via ERK5 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Isabelle Seidita,Ignazia Tusa,Matteo Prisinzano,Alessio Menconi,Francesca Cencetti,Silvia Vannuccini,Francesca Castiglione,Paola Bruni,Felice Petraglia,Caterina Bernacchioni,Elisabetta Rovida,Chiara Donati

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2300738 PubMed37170724

2023

Targeting L-Selectin Lymphocytes to Deliver Immunosuppressive Drug in Lymph Nodes for Durable Multiple Sclerosis Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Yipeng Zhao,Jie Zhang,Xi Cheng,Wenping Huang,Shishi Shen,Shilin Wu,Yiying Huang,Guangjun Nie,Hai Wang,Wei Qiu

Military Medical Research 10:13 PubMed36907884

2023

Pericytes protect rats and mice from sepsis-induced injuries by maintaining vascular reactivity and barrier function: implication of miRNAs and microvesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Sen Zhang,Yi-Yan Liu,Shuang-Shuang He,Dai-Qin Bao,Hong-Chen Wang,Jie Zhang,Xiao-Yong Peng,Jia-Tao Zang,Yu Zhu,Yue Wu,Qing-Hui Li,Tao Li,Liang-Ming Liu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2204961 PubMed36698265

2023

Reversing T Cell Dysfunction to Boost Glioblastoma Immunotherapy by Paroxetine-Mediated GRK2 Inhibition and Blockade of Multiple Checkpoints through Biomimetic Nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Wang,Hao Zhang,Yaobao Han,Qing Zheng,Hanghang Liu,Mengxiao Han,Zhen Li

Neural regeneration research 18:840-848 PubMed36204852

2022

Siponimod exerts neuroprotective effects on the retina and higher visual pathway through neuronal S1PR1 in experimental glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Devaraj Basavarajappa,Vivek Gupta,Nitin Chitranshi,Roshana Vander Wall,Rashi Rajput,Kanishka Pushpitha,Samridhi Sharma,Mehdi Mirzaei,Alexander Klistorner,Stuart L Graham
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com