Rabbit Recombinant Monoclonal S1P1/EDG1 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
ICC/IF | IP | Flow Cyt | WB | IHC-P | IHC-Fr | |
---|---|---|---|---|---|---|
Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Mouse | Not recommended | Tested | Not recommended | Tested | Tested | Not recommended |
Rat | Not recommended | Expected | Not recommended | Tested | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Rat | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis. Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury.
Edg1, Lpb1, Edg1, S1pr1, Lpb1, Sphingosine 1-phosphate receptor 1, S1P receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, Lysophospholipid receptor B1, Sphingosine 1-phosphate receptor Edg-1, S1P receptor Edg-1
Rabbit Recombinant Monoclonal S1P1/EDG1 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR23535-146
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
The S1P1 receptor also known as EDG1 is a G-protein-coupled receptor that plays a significant role in the sphingosine-1-phosphate (S1P) signaling pathway. With a molecular mass of approximately 43 kDa S1P1 is expressed in various tissues such as vascular endothelial cells immune cells and cardiac cells. The protein mediates the effects of sphingosine-1-phosphate a bioactive lipid that participates in various physiological processes.
S1P1 is involved in the regulation of immune cell trafficking and vascular stability. As a part of a receptor complex it activates intracellular signaling cascades that influence cell migration and vascular maturation. By binding to S1P S1P1 controls the egress of lymphocytes from lymphoid tissues indicating its important role in immune surveillance and response.
S1P1 serves as an important component in the S1P signaling pathway and the phosphoinositide 3-kinase (PI3K) pathway. In the S1P pathway it regulates endothelial cell barrier function and vascular maturation. In the PI3K pathway S1P1 influences cell survival and migration which relates it to protein kinases like Akt and other signaling molecules that further modify cellular responses.
S1P1 is implicated in multiple sclerosis (MS) and cancer progression. In MS S1P1 modulation affects lymphocyte trafficking which is the mechanism behind the action of fingolimod (FTY720) a therapeutic agent for the disease. S1P1 also connects to VEGF and other angiogenic factors in cancer where it influences tumor angiogenesis and metastasis. Understanding these connections highlights the potential for targeting S1P1 in therapeutic strategies for these conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID:19810093, 14688232).
Low expression: testis (PMID:9931453).
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 48 seconds
All lanes: Western blot - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse lung tissue lysate 20 at 20 µg
Lane 3: Mouse testis tissue lysate at 20 µg
Lane 4: bEnd.3 (mouse brain endothelioma) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 48 kDa
This data was developed using ab259902, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID:19810093, 14688232).
Low expression: testis (PMID:9931453).
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 48 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: testis (PMID:9931453).
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 48 seconds
All lanes: Western blot - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg
Lane 2: Rat testis tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 42 kDa
This data was developed using ab259902, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: testis (PMID:9931453).
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 48 seconds
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling S1P1/EDG1 with ab259902 at 1/100 (5.08 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining in mouse cerebrum. The section was incubated with Anti-IL-1 beta antibody [EPR23851-127] ab254360 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
S1P1/EDG1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab259902 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259902 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: ab259902 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259902 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5.5 seconds
Sample loaded onto lane 1 is non-boiled as boiling may cause protein aggregates.
All lanes: Immunoprecipitation - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)
Predicted band size: 43 kDa
Observed band size: 42 kDa
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling S1P1/EDG1 with ab259902 at 1/100 (5.08 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining in rat cerebrum. The section was incubated with Anti-IL-1 beta antibody [EPR23851-127] ab254360 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com