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AB235919

Anti-S1PR2 antibody

1

(1 Review)

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(8 Publications )

Rabbit Polyclonal S1PR2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human S1PR2 aa 250 to C-terminus.

View Alternative Names

EDG5, S1PR2, Sphingosine 1-phosphate receptor 2, S1P receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Sphingosine 1-phosphate receptor Edg-5, S1P receptor Edg-5

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-S1PR2 antibody (AB235919)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-S1PR2 antibody (AB235919)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for S1PR2 (green) using ab235919 at 1/200 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Counter stained with DAPI.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human S1PR2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95136

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S1PR2 also known as Sphingosine-1-phosphate receptor 2 is a G protein-coupled receptor with a mass of approximately 38 kDa. This receptor plays a role in cell signaling by binding to its ligand sphingosine-1-phosphate (S1P) which leads to various intracellular responses. S1PR2 is expressed in various tissues including the brain heart and immune cells where it affects physiological processes like cell proliferation migration and vascular stability.
Biological function summary

The receptor S1PR2 modulates the immune system and vascular function. It does not work alone but interacts with other S1P receptors to regulate the immune response and vascular tone. In immune cells S1PR2 influences cell movement and adhesion impacting mechanisms such as lymphocyte trafficking and immune modulation.

Pathways

S1PR2 is an important part of the sphingosine-1-phosphate signaling pathway. This pathway affects vascular and immune functions by interacting with downstream signaling molecules like Rho and Rac. S1PR2 also connects with other S1P receptors like S1PR1 and S1PR3 forming a network that controls cell motility and vascular health.

S1PR2 has implications in conditions like cancer and multiple sclerosis. In cancer S1PR2 affects tumor growth and metastasis through signaling pathways that control cell migration and angiogenesis. Its interaction with proteins like RhoA may contribute to cancer progression. In multiple sclerosis S1PR2 influences immune cell infiltration in the central nervous system adding complexity to immune-related disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P) (PubMed : 10617617, PubMed : 25274307). S1P is a bioactive lysophospholipid that elicits diverse physiological effects on most types of cells and tissues (PubMed : 10617617). When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis (PubMed : 10617617). Receptor for the chemokine-like protein FAM19A5 (PubMed : 29453251). Mediates the inhibitory effect of FAM19A5 on vascular smooth muscle cell proliferation and migration (By similarity). In lymphoid follicles, couples the binding of S1P to the activation of GNA13 and downstream inhibition of AKT activation leading to suppression of germinal center (GC) B cell growth and migration outside the GC niche.
See full target information S1PR2
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e70228 PubMed39622780

2024

Geraniin Alleviates Mouse Laser-Induced Choroidal Neovascularisation by Inhibiting Choroidal Endothelial Cell ACE2/Ang-(1-7)/MasR/IL-10 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyi Lu,Qi Cai,Lele Li,Jiayi Gu,Yuting Zhang,Haotian Sun,Hui Su,Lei Song

iScience 27:111290 PubMed39618500

2024

Broad and diverse roles of sphingosine-1-phosphate/sphingosine-1-phosphate receptors in the prostate.

Applications

Unspecified application

Species

Unspecified reactive species

Daoquan Liu,Jianmin Liu,Yan Li,Lu Du,Qingqiong Cao,Liang Yang,Yongying Zhou,Ping Chen,Yuming Guo,Guang Zeng,Michael E DiSanto,Weidong Hu,Xinhua Zhang

Journal of inflammation research 17:9103-9117 PubMed39583857

2024

Comparison of the Cecum Ligation and Puncture Method and the Intraperitoneal Lipopolysaccharide Injection Method for the Construction of a New-Onset Atrial Fibrillation Model of Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuwen Ling,Jun Shen,Junqing Liang,Kai Yang,Jianzhong Yang

Lipids in health and disease 22:211 PubMed38041133

2023

A comprehensive multiomics approach reveals that high levels of sphingolipids in cardiac cachexia adipose tissue are associated with inflammatory and fibrotic changes.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwei Qu,Yong Wang,Tao Wu,Xue Liu,Huaizhe Wang,Dufang Ma

Frontiers in cellular and infection microbiology 12:1011378 PubMed36339341

2022

infection induces hepatobiliary injury disturbing sphingolipid metabolism and activating sphingosine 1-phosphate receptor 2.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Xin Liu,Man Liu,Guo-Zhi Yu,Qian-Qian Zhao,Jian-Ling Wang,Yan-Hong Sun,Stephane Koda,Beibei Zhang,Qian Yu,Chao Yan,Ren-Xian Tang,Zhi-Hua Jiang,Kui-Yang Zheng

Redox biology 56:102466 PubMed36113340

2022

Regulation of CyR61 expression and release by 3-mercaptopyruvate sulfurtransferase in colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly Ascenção,Bassma Lheimeur,Csaba Szabo

Experimental and therapeutic medicine 23:3 PubMed34815755

2021

Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Lin,Chunli Long,Zhengang Wang,Ronghui Wang,Wei Shi,Jiwei Qiu,Junlin Mo,Yongxiang Xie

Journal of cellular physiology 235:3864-3873 PubMed31603252

2019

Sphingosine-1-phosphate receptor 2 modulates pain sensitivity by suppressing the ROS-RUNX3 pathway in a rat model of neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yinyu Li,Huanli Li,Jinsong Han
View all publications
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Product promise

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