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Rabbit Recombinant Monoclonal S1PR2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-S1PR2 antibody [EPR27141-13] - BSA and Azide free (AB306563), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytICC/IFIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P) (PubMed:10617617). S1P is a bioactive lysophospholipid that elicits diverse physiological effects on most types of cells and tissues (PubMed:10617617). When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis (PubMed:10617617). Receptor for the chemokine-like protein FAM19A5 (PubMed:29453251). Mediates the inhibitory effect of FAM19A5 on vascular smooth muscle cell proliferation and migration (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal S1PR2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR27141-13

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

S1PR2 also known as Sphingosine-1-phosphate receptor 2 is a G protein-coupled receptor with a mass of approximately 38 kDa. This receptor plays a role in cell signaling by binding to its ligand sphingosine-1-phosphate (S1P) which leads to various intracellular responses. S1PR2 is expressed in various tissues including the brain heart and immune cells where it affects physiological processes like cell proliferation migration and vascular stability.

Biological function summary

The receptor S1PR2 modulates the immune system and vascular function. It does not work alone but interacts with other S1P receptors to regulate the immune response and vascular tone. In immune cells S1PR2 influences cell movement and adhesion impacting mechanisms such as lymphocyte trafficking and immune modulation.

Pathways

S1PR2 is an important part of the sphingosine-1-phosphate signaling pathway. This pathway affects vascular and immune functions by interacting with downstream signaling molecules like Rho and Rac. S1PR2 also connects with other S1P receptors like S1PR1 and S1PR3 forming a network that controls cell motility and vascular health.

Associated diseases and disorders

S1PR2 has implications in conditions like cancer and multiple sclerosis. In cancer S1PR2 affects tumor growth and metastasis through signaling pathways that control cell migration and angiogenesis. Its interaction with proteins like RhoA may contribute to cancer progression. In multiple sclerosis S1PR2 influences immune cell infiltration in the central nervous system adding complexity to immune-related disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-S1PR2 antibody [EPR27141-13] - BSA and Azide free (ab306563), expandable thumbnail

    Western blot - Anti-S1PR2 antibody [EPR27141-13] - BSA and Azide free (ab306563)

    This data was developed using Anti-S1PR2 antibody [EPR27141-13] ab306562, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: HeLa, LoVo, HUVEC (PMID: 32088343, PMID: 15618544).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-S1PR2 antibody [EPR27141-13] (Anti-S1PR2 antibody [EPR27141-13] ab306562) at 1/1000 dilution

    Lane 1: Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 39 kDa

    Exposure time: 125s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: HeLa, LoVo, HUVEC (PMID: 32088343, PMID: 15618544).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com