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AB244279

Anti-S2P antibody

4

(5 Reviews)

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(0 Publication)

Rabbit Polyclonal S2P antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MBTPS2 aa 250-400.

View Alternative Names

S2P, MBTPS2, Membrane-bound transcription factor site-2 protease, Endopeptidase S2P, Sterol regulatory element-binding proteins intramembrane protease, SREBPs intramembrane protease

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S2P antibody (AB244279)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S2P antibody (AB244279)

Formalin-fixed, paraffin-embedded human rectum tissue stained for SP2 using ab244279 at 1/20 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MBTPS2 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

O43462

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S2P also known as Site-2 protease or IMP1 is an intramembrane zinc metalloprotease with a mass around 52 kDa. It carries out the proteolytic cleavage of membrane-bound transcription factors. S2P resides in cellular locations like the endoplasmic reticulum membrane and is highly expressed in tissues such as the liver and muscle. This protease acts after the initial cleavage by Site-1 protease (S1P) playing an important role in regulated intramembrane proteolysis.
Biological function summary

S2P cleaves specific substrates involved in lipid metabolism and stress response. This protease is an integral component of the sterol-regulatory element-binding proteins (SREBPs) complex. The cleavage by S2P releases the active form of SREBPs which translocates into the nucleus to regulate genes responsible for cholesterol and fatty acid synthesis. S2P ensures that these transcription factors maintain lipid homeostasis and adapt to cellular environment changes.

Pathways

S2P significantly influences cholesterol biosynthesis and unfolded protein response pathways. In the cholesterol biosynthesis pathway it regulates the activation of SREBPs which in turn controls cholesterol and lipid biosynthesis genes. Additionally in the unfolded protein response pathway it interacts with proteins such as ATF6 to help manage endoplasmic reticulum stress. These interactions highlight S2P's pivotal role in maintaining cellular balance under varying conditions.

S2P dysfunction can lead to conditions like hypercholesterolemia and metabolic syndrome. Impaired S2P activity disrupts normal cholesterol regulation potentially causing elevated cholesterol levels and related cardiovascular diseases. S2P also connects to ATF6 in response to stress and any disturbances might influence metabolic pathways contributing to metabolic syndrome. Understanding the role of S2P in these diseases offers insights into potential therapeutic approaches for managing lipid-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Zinc metalloprotease that mediates intramembrane proteolysis of proteins such as ATF6, ATF6B, SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed : 10805775, PubMed : 11163209). Catalyzes the second step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 : cleaves SREBPs within the first transmembrane segment, thereby releasing the N-terminal segment with a portion of the transmembrane segment attached (PubMed : 10805775, PubMed : 27380894, PubMed : 9659902). Mature N-terminal SREBP fragments shuttle to the nucleus and activate gene transcription (PubMed : 10805775, PubMed : 27380894, PubMed : 9659902). Also mediates the second step in the proteolytic activation of the cyclic AMP-dependent transcription factor ATF-6 (ATF6 and ATF6B) (PubMed : 11163209). Involved in intramembrane proteolysis during bone formation (PubMed : 27380894). In astrocytes and osteoblasts, upon DNA damage and ER stress, mediates the second step of the regulated intramembrane proteolytic activation of the transcription factor CREB3L1, leading to the inhibition of cell-cycle progression (PubMed : 16417584).
See full target information MBTPS2
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Product promise

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