JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB236651

Anti-S31 antibody

3

(1 Review)

|

(1 Publication)

Rabbit Polyclonal S31 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human 5-hydroxytryptamine receptor 1E aa 200-300.

View Alternative Names

5-hydroxytryptamine receptor 1E, 5-HT-1E, 5-HT1E, S31, Serotonin receptor 1E, HTR1E

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-S31 antibody (AB236651)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-S31 antibody (AB236651)

PC-3 (human prostate adenocarcinoma cell line) cells stained for S31 (green) using ab236651 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S31 antibody (AB236651)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S31 antibody (AB236651)

Paraffin-embedded human kidney tissue stained for S31 using ab236651 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S31 antibody (AB236651)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S31 antibody (AB236651)

Paraffin-embedded human lung cancer tissue stained for S31 using ab236651 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-S31 antibody (AB236651)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-S31 antibody (AB236651)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for S31 (green) using ab236651 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S31 antibody (AB236651)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S31 antibody (AB236651)

Paraffin-embedded human testis tissue stained for S31 using ab236651 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-S31 antibody (AB236651)
  • WB

Supplier Data

Western blot - Anti-S31 antibody (AB236651)

All lanes:

Western blot - Anti-S31 antibody (ab236651) at 1/500 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human 5-hydroxytryptamine receptor 1E aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

P28566

style
left-column

Alternative Products

Primary Antibodies

AB154813

Anti-S31 antibody [EPR10789(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-S31 antibody [EPR10789(B)] (AB154813)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB140767

Anti-GPR83 antibody - Extracellular domain

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR83 antibody - Extracellular domain (AB140767)

  • 0
  • 1
Primary Antibodies

AB133323

Anti-14-3-3 antibody [EPR6379]

RabMAb|Recombinant

Western blot - Anti-14-3-3 antibody [EPR6379] (AB133323)

  • 0
  • 0
Primary Antibodies

AB237050

Anti-CBWD2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CBWD2 antibody (AB237050)

  • 0
  • 0
Primary Antibodies

AB250223

Anti-ZNF529 antibody [EPR13674] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-ZNF529 antibody [EPR13674] - BSA and Azide free (AB250223)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S31 protein also known as the Influenza A virus proton channel M2 facilitates the pH regulation during viral infection. It functions as a transmembrane ion channel and plays an essential role in the viral uncoating process. S31 is a relatively small protein with a mass of roughly 97 amino acids. It is primarily expressed in the membranes of infected host cells during the lifecycle of the Influenza A virus contributing to the acidification necessary for viral RNA release.
Biological function summary

S31 is integral to the influenza virus replication process. It does not work alone but interacts with other viral proteins to form a functional ion channel complex. This complex is critical in modulating the internal pH of viral particles which subsequently assists in viral genome release into the host cell cytoplasm. The activity of S31 is pivotal for the successful entry of the virus into the host cell and its replication cycle.

Pathways

The functional role of S31 connects to the vesicular acidification and M2-mediated ion transport pathways. It is closely associated with the M1 protein of the Influenza A virus contributing to the virus's ability to hijack cellular machinery. These pathways facilitate the release of viral ribonucleoproteins into the cytoplasm by altering the endosomal pH important for the continuation of the viral replication process.

S31 is directly associated with Influenza A infections causing seasonal flu outbreaks. Mutations in the S31 protein can lead to resistance to antiviral drugs such as amantadine complicating treatment strategies. It connects to the M1 protein through the viral lifecycle impacting the efficiency of viral assembly and dissemination. Understanding the mechanism of S31 opens avenues for developing targeted therapeutics that inhibit viral propagation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 14744596, PubMed : 1513320, PubMed : 1608964, PubMed : 1733778, PubMed : 21422162, PubMed : 33762731). Also functions as a receptor for various alkaloids and psychoactive substances (PubMed : 14744596, PubMed : 1513320, PubMed : 1608964, PubMed : 1733778, PubMed : 21422162, PubMed : 33762731). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 14744596, PubMed : 1513320, PubMed : 1608964, PubMed : 1733778, PubMed : 21422162, PubMed : 33762731). HTR1E is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission by inhibiting adenylate cyclase activity (PubMed : 33762731, PubMed : 35610220).
See full target information 5-hydroxytryptamine receptor 1E
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:3705-3725 PubMed40520022

2025

ITLN1 exacerbates Crohn's colitis by driving ZBP1-dependent PANoptosis in intestinal epithelial cells through antagonizing TRIM8-mediated CAPN2 ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhao,Yujiang Li,Pu Ying,Yan Zhou,Ziwei Xu,Dongmei Wang,Honggang Wang,Liming Tang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com