AB154813

Anti-S31 antibody [EPR10789(B)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal S31 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

5-hydroxytryptamine receptor 1E, 5-HT-1E, 5-HT1E, S31, Serotonin receptor 1E, HTR1E

2 Images

Flow Cytometry (Intracellular) - Anti-S31 antibody [EPR10789(B)] (AB154813)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-S31 antibody [EPR10789(B)] (AB154813)

Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells labeling S31 with ab154813 at 1/10 dilution (red), compared to a rabbit IgG negative control antibody (green).

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Western blot - Anti-S31 antibody [EPR10789(B)] (AB154813)

All lanes:

Western blot - Anti-S31 antibody [EPR10789(B)] (ab154813) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 42 kDa

false

Carrier free

Anti-S31 antibody [EPR10789(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10789(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S31 protein also known as the Influenza A virus proton channel M2 facilitates the pH regulation during viral infection. It functions as a transmembrane ion channel and plays an essential role in the viral uncoating process. S31 is a relatively small protein with a mass of roughly 97 amino acids. It is primarily expressed in the membranes of infected host cells during the lifecycle of the Influenza A virus contributing to the acidification necessary for viral RNA release.

Biological function summary

S31 is integral to the influenza virus replication process. It does not work alone but interacts with other viral proteins to form a functional ion channel complex. This complex is critical in modulating the internal pH of viral particles which subsequently assists in viral genome release into the host cell cytoplasm. The activity of S31 is pivotal for the successful entry of the virus into the host cell and its replication cycle.

Pathways

The functional role of S31 connects to the vesicular acidification and M2-mediated ion transport pathways. It is closely associated with the M1 protein of the Influenza A virus contributing to the virus's ability to hijack cellular machinery. These pathways facilitate the release of viral ribonucleoproteins into the cytoplasm by altering the endosomal pH important for the continuation of the viral replication process.

S31 is directly associated with Influenza A infections causing seasonal flu outbreaks. Mutations in the S31 protein can lead to resistance to antiviral drugs such as amantadine complicating treatment strategies. It connects to the M1 protein through the viral lifecycle impacting the efficiency of viral assembly and dissemination. Understanding the mechanism of S31 opens avenues for developing targeted therapeutics that inhibit viral propagation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 14744596, PubMed : 1513320, PubMed : 1608964, PubMed : 1733778, PubMed : 21422162, PubMed : 33762731). Also functions as a receptor for various alkaloids and psychoactive substances (PubMed : 14744596, PubMed : 1513320, PubMed : 1608964, PubMed : 1733778, PubMed : 21422162, PubMed : 33762731). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 14744596, PubMed : 1513320, PubMed : 1608964, PubMed : 1733778, PubMed : 21422162, PubMed : 33762731). HTR1E is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission by inhibiting adenylate cyclase activity (PubMed : 33762731, PubMed : 35610220).

See full target information 5-hydroxytryptamine receptor 1E

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37298523

2023

miRNA-132/212 Deficiency Disrupts Selective Corticosterone Modulation of Dorsal vs. Ventral Hippocampal Metaplasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Shima Kouhnavardi,Maureen Cabatic,M Carmen Mañas-Padilla,Marife-Astrid Malabanan,Tarik Smani,Ana Cicvaric,Edison Alejandro Muñoz Aranzalez,Xaver Koenig,Ernst Urban,Gert Lubec,Estela Castilla-Ortega,Francisco J Monje

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-S31 antibody [EPR10789(B)]

Flow Cytometry (Intracellular) - Anti-S31 antibody [EPR10789(B)] (AB154813)

Western blot - Anti-S31 antibody [EPR10789(B)] (AB154813)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise