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AB249120

Anti-S31 antibody [EPR10789(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal S31 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

5-hydroxytryptamine receptor 1E, 5-HT-1E, 5-HT1E, S31, Serotonin receptor 1E, HTR1E

1 Images
Western blot - Anti-S31 antibody [EPR10789(B)] - BSA and Azide free (AB249120)
  • WB

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Western blot - Anti-S31 antibody [EPR10789(B)] - BSA and Azide free (AB249120)

This data was developed using ab154813, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-S31 antibody [EPR10789(B)] (<a href='/en-us/products/primary-antibodies/s31-antibody-epr10789b-ab154813'>ab154813</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 42 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10789(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab249120 is the carrier-free version of ab154813.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S31 protein also known as the Influenza A virus proton channel M2 facilitates the pH regulation during viral infection. It functions as a transmembrane ion channel and plays an essential role in the viral uncoating process. S31 is a relatively small protein with a mass of roughly 97 amino acids. It is primarily expressed in the membranes of infected host cells during the lifecycle of the Influenza A virus contributing to the acidification necessary for viral RNA release.
Biological function summary

S31 is integral to the influenza virus replication process. It does not work alone but interacts with other viral proteins to form a functional ion channel complex. This complex is critical in modulating the internal pH of viral particles which subsequently assists in viral genome release into the host cell cytoplasm. The activity of S31 is pivotal for the successful entry of the virus into the host cell and its replication cycle.

Pathways

The functional role of S31 connects to the vesicular acidification and M2-mediated ion transport pathways. It is closely associated with the M1 protein of the Influenza A virus contributing to the virus's ability to hijack cellular machinery. These pathways facilitate the release of viral ribonucleoproteins into the cytoplasm by altering the endosomal pH important for the continuation of the viral replication process.

S31 is directly associated with Influenza A infections causing seasonal flu outbreaks. Mutations in the S31 protein can lead to resistance to antiviral drugs such as amantadine complicating treatment strategies. It connects to the M1 protein through the viral lifecycle impacting the efficiency of viral assembly and dissemination. Understanding the mechanism of S31 opens avenues for developing targeted therapeutics that inhibit viral propagation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 14744596, PubMed : 1513320, PubMed : 1608964, PubMed : 1733778, PubMed : 21422162, PubMed : 33762731). Also functions as a receptor for various alkaloids and psychoactive substances (PubMed : 14744596, PubMed : 1513320, PubMed : 1608964, PubMed : 1733778, PubMed : 21422162, PubMed : 33762731). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 14744596, PubMed : 1513320, PubMed : 1608964, PubMed : 1733778, PubMed : 21422162, PubMed : 33762731). HTR1E is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission by inhibiting adenylate cyclase activity (PubMed : 33762731, PubMed : 35610220).
See full target information 5-hydroxytryptamine receptor 1E
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Product promise

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