Anti-S6K1 antibody [E343] - BSA and Azide free

13 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Immunohistochemical analysis of rat brain tissue labeling S6K1 with Anti-S6K1 antibody [E343] ab32529 at 1/500 dilution (4.4 μg/mL). The secondary antibody used was ImmunoHistoProbe one step HRP Polymer (ready to use). Secondary antibody only control-PBS instead of the primary antibody. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The tissue was counterstained with Hematoxylin.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Immunohistochemical analysis of mouse testis tissue labeling S6K1 with Anti-S6K1 antibody [E343] ab32529 at 1/500 dilution (4.4 μg/mL). The secondary antibody used was ImmunoHistoProbe one step HRP Polymer (ready to use). Secondary antibody only control-PBS instead of the primary antibody. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The tissue was counterstained with Hematoxylin.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Immunohistochemical analysis of Human breast cancer tissue labeling S6K1 with Anti-S6K1 antibody [E343] ab32529 at 1/500 dilution (4.4 μg/mL). The secondary antibody used was ImmunoHistoProbe one step HRP Polymer (ready to use). Secondary antibody only control-PBS instead of the primary antibody. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The tissue was counterstained with Hematoxylin.
  
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).

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  Flow Cytometry (Intracellular) - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Intracellular Flow Cytometry analysis of C6 (Rat glial tumor glial cell) cells labelling with Anti-S6K1 antibody [E343] ab32529 (purified) at 1/2200 dilution (1 μg/mL) (red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluorr^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - 90% methanol . Unlabeled control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).

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  Immunoprecipitation - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Lane 1: Neuro2a (Mouse neuroblastoma neuroblast) whole cell lysate, 10μg
  Lane 2: Neuro2a whole cell lysate 350μg and Anti-S6K1 antibody [E343] ab32529, 2μg
  Lane 3: Neuro2a cell lysate, 350μg and rabbit IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730), 2μg
  
  Purified Anti-S6K1 antibody [E343] ab32529 immunoprecipitating S6K1 in HEK293T cell lysates. Primary antibody was used at a 1:500 dilution (4.4 μg/ml). For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
  

  Blocking and diluting buffer used: 5% NFDM/TBST.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).

  All lanes: Immunoprecipitation - Anti-S6K1 antibody [E343] (Anti-S6K1 antibody [E343] ab32529)

  Predicted band size: 59 kDa

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  Flow Cytometry (Intracellular) - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Intracellular Flow Cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labelling with Anti-S6K1 antibody [E343] ab32529 (purified) at 1/2200 dilution (1 μg/mL) (red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluorr^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - 90% methanol . Unlabeled control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).

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  Immunoprecipitation - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Lane 1: HEK293T (Human embryonic kidney epithelial cell) whole cell lysate, 10μg
  Lane 2: HEK293T whole cell lysate, 10μg and Anti-S6K1 antibody [E343] ab32529, 2μg
  Lane 3: HEK293T cell lysate, 350μg and rabbit IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) , 2μg
  
  Purified Anti-S6K1 antibody [E343] ab32529 immunoprecipitating S6K1 in HEK293T cell lysates. Primary antibody was used at a 1:500 dilution (4.4 μg/ml). For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
  
  Blocking and diluting buffer used: 5% NFDM/TBST.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).

  All lanes: Immunoprecipitation - Anti-S6K1 antibody [E343] (Anti-S6K1 antibody [E343] ab32529)

  Predicted band size: 59 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Immunocytochemistry/Immunofluorescence analysis of C6 cells (Rat glial tumor glial cell)  labelling S6K1 with Anti-S6K1 antibody [E343] ab32529 at a dilution of 1:200, 11.1 µg/ml. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. A 1:1000 dilution (2μg/ml) was used for the secondary antibody Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). The cells were co-stained with 1:200, 2.5μg/ml with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Nuclei counterstained with DAPI (blue). Control: 1:1000 dilution.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).

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  Immunocytochemistry/ Immunofluorescence - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Immunocytochemistry/Immunofluorescence analysis of NIH/3T3 (Mouse embryonic fibroblast) labelling with Anti-S6K1 antibody [E343] ab32529 at a dilution of 1:200, 11.1 μg/ml. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. A 1:1000 dilution (2μg/ml) was used for the secondary antibody Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). The cells were co-stained at 1:200 dilution, 2.5μg/ml with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Nuclei counterstained with DAPI (blue). Control: 1:1000 dilution.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).

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  Immunocytochemistry/ Immunofluorescence - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Immunocytochemistry/Immunofluorescence analysis of MCF 7 (Human breast adenocarcinoma epithelial cell) labeling S6K1 with Anti-S6K1 antibody [E343] ab32529 at a dilution of 1:200, 11.1 ug/ml. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. A dilution of 1/1000 (2μg/ml)  was used for the secondary antibodyGoat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). The cells were co-stained at 1:200 dilution, 2.5μg/ml with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) . Nuclei counterstained with DAPI (blue). Control: 1:1000 dilution.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).

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  Immunocytochemistry/ Immunofluorescence - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling S6K1 with Anti-S6K1 antibody [E343] ab32529 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).    Confocal image showing cytoplamic staining on HeLa cell line. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).

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  Flow Cytometry (Intracellular) - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Overlay histogram showing HeLa cells stained with Anti-S6K1 antibody [E343] ab32529 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-S6K1 antibody [E343] ab32529, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).
  

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  Flow Cytometry (Intracellular) - Anti-S6K1 antibody [E343] - BSA and Azide free (ab203558)

  Intracellular Flow Cytometry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling with Anti-S6K1 antibody [E343] ab32529 (purified) at 1/2200 dilution (1 μg/mL) (red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluorr^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - 90% methanol . Unlabeled control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S6K1 antibody [E343] ab32529).