AB207399

Anti-S6K1 (phospho T449) antibody

4

(1 Review)

|

(14 Publications)

Rabbit Polyclonal S6K1 phospho T449 antibody. Suitable for WB and reacts with Arabidopsis thaliana samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Arabidopsis thaliana ATPK1 phospho T449.

View Alternative Names

ATPK6, S6K1, At3g08730, F17O14.20, ATPK1, Serine/threonine-protein kinase AtPK1/AtPK6, Ribosomal-protein S6 kinase homolog 1

1 Images

Western blot - Anti-S6K1 (phospho T449) antibody (AB207399)

WB

Supplier Data

Western blot - Anti-S6K1 (phospho T449) antibody (AB207399)

All lanes:

Western blot - Anti-S6K1 (phospho T449) antibody (ab207399) at 1/1000 dilution

Lane 1:

Control peptide

Lane 2:

S6K1 Phosphopeptide (T449) used as blocking peptide

Lane 3:

S6K1 Non-phosphopeptide used as blocking peptide

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Arabidopsis thaliana

Applications

WB

Immunogen

Synthetic Peptide within Arabidopsis thaliana ATPK1 phospho T449. The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab207399 is specific for the ~53k S6K1 protein phosphorylated at Thr 449. The immunolabeling of the S6K1 band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling.

style

left-column

Alternative Products

Primary Antibodies

AB32359

Anti-S6K1 antibody [E175]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-S6K1 antibody [E175] (AB32359)

3
4

Primary Antibodies

AB32529

Anti-S6K1 antibody [E343]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody [E343] (AB32529)

1
1

Primary Antibodies

AB203558

Anti-S6K1 antibody [E343] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody [E343] - BSA and Azide free (AB203558)

0
0

Primary Antibodies

AB60948

Anti-S6K1 (phospho T389 + T412) antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho T389 + T412) antibody (AB60948)

1
1

Primary Antibodies

AB239810

Anti-S6K1 antibody [E175] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-S6K1 antibody [E175] - BSA and Azide free (AB239810)

0
0

Complementary Products

Primary Antibodies

AB32529

Anti-S6K1 antibody [E343]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody [E343] (AB32529)

1
1

Primary Antibodies

AB8580

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

5
90

Primary Antibodies

AB32024

Anti-eIF4EBP1 antibody [Y329]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-eIF4EBP1 antibody [Y329] (AB32024)

0
0

Primary Antibodies

AB109268

Anti-mTOR (phospho S2448) antibody [EPR426(2)]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

4
6

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Arabidopsis thaliana": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab207399 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage buffer

pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.87% Sodium chloride, 0.24% HEPES

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S6K1 also known as p70S6K is a serine/threonine kinase involved in protein synthesis. The protein has a molecular weight of approximately 70 kDa. Scientists often examine the presence of S6K1 in various tissues as it is expressed ubiquitously in eukaryotic cells. This kinase plays an instrumental role in the regulation of cell growth proliferation and survival by phosphorylating ribosomal protein S6 and other targets. Researchers explore various cellular contexts where S6K1 activity is implicated due to its widespread expression and function.

Biological function summary

S6K1 functions as a downstream effector of the mammalian target of rapamycin (mTOR) complex specifically mTORC1. S6K1 interacts with several protein complexes enhancing its regulatory capacity in cellular activities. It modulates protein synthesis by phosphorylating substrates involved in translation. By controlling these processes S6K1 aids in cell size regulation and energy metabolism which are critical for maintaining cellular homeostasis and adaptation to nutrient availability.

Pathways

S6K1 plays an essential role in the mTOR signaling pathway a central regulator of cell growth and metabolism. S6K1 functions in close connection with proteins such as mTOR and Raptor within this pathway. Additionally it is involved in the insulin signaling pathway where it works with proteins like insulin receptor substrate (IRS). Both pathways highlight S6K1's role in nutrient sensing and response emphasizing its significance in energy and protein homeostasis.

S6K1 is associated with conditions such as cancer and type 2 diabetes. Dysregulation of S6K1 activity can lead to abnormal cell proliferation contributing to oncogenesis in various cancer types. Specifically altered signaling through pathways involving mTOR and Akt proteins connects S6K1 to cancer progression. In type 2 diabetes S6K1 affects insulin sensitivity where overactive S6K1 signaling leads to insulin resistance. Here interactions with insulin receptor substrate proteins highlight its influence on metabolic disorders underlining its critical role in disease pathology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Downstream effector of TOR signaling pathway involved in osmotic stress response (PubMed : 20683442). Could be involved in the control of plant growth and development (PubMed : 20683442). Phosphorylates the ribosomal proteins P14, P16 and S6 (PubMed : 20683442). Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway (PubMed : 20683442). Mediates the phosphorylation of MRFs (e.g. MRF1) (PubMed : 29084871).

See full target information ATPK1 phospho T449

style

left-column

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 120:e2316011120 PubMed37967217

2023

TORC pathway intersects with a calcium sensor kinase network to regulate potassium sensing in .

Applications

Unspecified application

Species

Unspecified reactive species

Kun-Lun Li,Hui Xue,Ren-Jie Tang,Sheng Luan

The EMBO journal 42:e111273 PubMed37021425

2023

TOR acts as a metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thaliana.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Stitz,David Kuster,Maximilian Reinert,Mikhail Schepetilnikov,Béatrice Berthet,Jazmin Reyes-Hernández,Denis Janocha,Anthony Artins,Marc Boix,Rossana Henriques,Anne Pfeiffer,Jan Lohmann,Emmanuel Gaquerel,Alexis Maizel

Current biology : CB 33:1778-1786.e5 PubMed36963384

2023

ARSK1 activates TORC1 signaling to adjust growth to phosphate availability in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Huikyong Cho,Michael Banf,Zaigham Shahzad,Jelle Van Leene,Flavia Bossi,Sandrine Ruffel,Nadia Bouain,Pengfei Cao,Gabiel Krouk,Geert De Jaeger,Benoit Lacombe,Federica Brandizzi,Seung Y Rhee,Hatem Rouached

The New phytologist 238:169-185 PubMed36716782

2023

Cell surface receptor kinase FERONIA linked to nutrient sensor TORC signaling controls root hair growth at low temperature linked to low nitrate in Arabidopsis thaliana.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Martínez Pacheco,Limei Song,Lenka Kuběnová,Miroslav Ovečka,Victoria Berdion Gabarain,Juan Manuel Peralta,Tomás Urzúa Lehuedé,Miguel Angel Ibeas,Martiniano M Ricardi,Sirui Zhu,Yanan Shen,Mikhail Schepetilnikov,Lyubov A Ryabova,José M Alvarez,Rodrigo A Gutierrez,Guido Grossmann,Jozef Šamaj,Feng Yu,José M Estevez

Journal of experimental botany 74:1420-1431 PubMed36515098

2022

TOR represses stress responses through global regulation of H3K27 trimethylation in plants.

Applications

Unspecified application

Species

Unspecified reactive species

Yihan Dong,Veli V Uslu,Alexandre Berr,Gaurav Singh,Csaba Papdi,Victor A Steffens,Thierry Heitz,Lyubov A Ryabova

Nature communications 13:4009 PubMed35817801

2022

Translational fidelity and growth of Arabidopsis require stress-sensitive diphthamide biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongliang Zhang,Julia Quintana,Koray Ütkür,Lorenz Adrian,Harmen Hawer,Klaus Mayer,Xiaodi Gong,Leonardo Castanedo,Anna Schulten,Nadežda Janina,Marcus Peters,Markus Wirtz,Ulrich Brinkmann,Raffael Schaffrath,Ute Krämer

The Plant journal : for cell and molecular biology 109:1229-1248 PubMed34902186

2021

The UPR regulator IRE1 promotes balanced organ development by restricting TOR-dependent control of cellular differentiation in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Evan Angelos,Federica Brandizzi

iScience 24:103260 PubMed34765910

2021

TOR senses and regulates spermidine metabolism during seedling establishment and growth in maize and Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Kenia Salazar-Díaz,Yihan Dong,Csaba Papdi,Ernesto Miguel Ferruzca-Rubio,Grecia Olea-Badillo,Lyubov A Ryabova,Tzvetanka D Dinkova

The Plant cell 33:1615-1632 PubMed33793860

2021

TOR coordinates nucleotide availability with ribosome biogenesis in plants.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Busche,M Regina Scarpin,Robert Hnasko,Jacob O Brunkard

Science advances 6: PubMed33087356

2020

HSB-1/HSF-1 pathway modulates histone H4 in mitochondria to control mtDNA transcription and longevity.

Applications

Unspecified application

Species

Unspecified reactive species

Surojit Sural,Chung-Yi Liang,Feng-Yung Wang,Tsui-Ting Ching,Ao-Lin Hsu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com