AB4455

Anti-SA1 antibody

Name: Anti-SA1 antibody (ab4455) | Abcam
Brand: Abcam Limited
SKU: AB4455
Price: 585 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Goat Polyclonal SA1 antibody. Suitable for ICC, IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human STAG1 aa 1-100.

View Alternative Names

SA1, SCC3, STAG1, Cohesin subunit SA-1, SCC3 homolog 1, Stromal antigen 1

4 Images

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Immunocytochemistry - Anti-SA1 antibody (AB4455)

Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4455 at 1µg/ml.

Supplier Data

Immunoprecipitation - Anti-SA1 antibody (AB4455)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Lane 1 : ab4455 used for IP at 6 μg per reaction.

Lane 2 : Control IgG.

For blotting immunoprecipitated SA1, ab4455 was used at 0.1 μg/ml.

All lanes:

Immunoprecipitation - Anti-SA1 antibody (ab4455)

Predicted band size: 144 kDa

false

Exposure time: 30s

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Western blot - Anti-SA1 antibody (AB4455)

Whole cell lysate (15 μg) from HeLa, MCF-7, HEK-293T, TCMK-1, and NIH/3T3 cells prepared using NETN lysis buffer.

ab4455 used for WB at 0.1 μg/ml.

All lanes:

Western blot - Anti-SA1 antibody (ab4455)

Predicted band size: 144 kDa

false

Exposure time: 30s

Unknown

Western blot - Anti-SA1 antibody (AB4455)

Nuclear Extract from HeLa cells (~5 x 10⁵ cells).

ab4455 at 0.5 μg/ml.

Detection : ECL with 15 second exposure.

Nuclear Extract from HeLa cells (~5 x 105 cells).

ab4455 at 0.5 µg/ml.

Detection : ECL with 15 second exposure.

All lanes:

Western blot - Anti-SA1 antibody (ab4455)

Predicted band size: 144 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB, ICC

applications

Immunogen

Synthetic Peptide within Human STAG1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q8WVM7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/500 - 1/2000", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rhesus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SA1 also known as Stromal Antigen 1 or cohesin subunit SA-1 plays a role in chromosome segregation and sister chromatid cohesion. It has a molecular weight of approximately 130 kDa. SA1 shows expression in various tissues with notable levels in the nucleus of dividing cells. The protein functions as a part of the cohesin complex contributing to its role in regulating the structure and function of chromosomes.

Biological function summary

SA1 works within the cohesin complex alongside other subunits such as SMC1 SMC3 and Rad21. This complex maintains sister chromatid cohesion ensuring proper chromosome alignment and separation during mitosis and meiosis. SA1's interaction within the cohesin complex helps modulate its ability to regulate transcription DNA repair and chromosome organization.

Pathways

SA1 participates in the cohesin pathway which is essential for cell cycle regulation and genomic stability. SA1 interacts with other proteins such as CTCF to facilitate chromosomal loop formation influencing gene expression. Additionally it operates within the DNA damage response pathway aiding in the repair processes by coordinating cohesin-dependent repair mechanisms and maintaining genomic stability.

SA1 has implications in Cornelia de Lange Syndrome (CdLS) and certain cancers. Mutations or dysregulation of SA1 can lead to CdLS a developmental disorder characterized by growth abnormalities and cognitive challenges where Rad21 also plays a role. Moreover altered SA1 expression or function associates with tumorigenesis as its role in chromosome segregation and stability is critical in avoiding aneuploidy and chromosomal instability typical in cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

See full target information STAG1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37010886

2023

Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading.

Applications

Unspecified application

Species

Unspecified reactive species

Hayley Porter,Yang Li,Maria Victoria Neguembor,Manuel Beltran,Wazeer Varsally,Laura Martin,Manuel Tavares Cornejo,Dubravka Pezić,Amandeep Bhamra,Silvia Surinova,Richard G Jenner,Maria Pia Cosma,Suzana Hadjur

Molecular biology of the cell 32:1-14 PubMed33175657

2020

EWSR1 affects PRDM9-dependent histone 3 methylation and provides a link between recombination hotspots and the chromosome axis protein REC8.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Tian,Timothy Billings,Petko M Petkov

PLoS genetics 10:e1004413 PubMed24992337

2014

Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes.

Applications

Species

Mouse

Jessica Hopkins,Grace Hwang,Justin Jacob,Nicklas Sapp,Rick Bedigian,Kazuhiro Oka,Paul Overbeek,Steve Murray,Philip W Jordan

Molecular and cellular biology 31:2174-83 PubMed21444719

2011

Specific sites in the C terminus of CTCF interact with the SA2 subunit of the cohesin complex and are required for cohesin-dependent insulation activity.

Applications

Species

Human

Tiaojiang Xiao,Julie Wallace,Gary Felsenfeld

Journal of cell science 123:660-70 PubMed20124417

2010

Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion.

Applications

IP, WB

Species

Human, Human

Adam R Leman,Chiaki Noguchi,Candice Y Lee,Eishi Noguchi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SA1 antibody

Immunocytochemistry - Anti-SA1 antibody (AB4455)

Immunoprecipitation - Anti-SA1 antibody (AB4455)

Western blot - Anti-SA1 antibody (AB4455)

Western blot - Anti-SA1 antibody (AB4455)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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