JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB4457

Anti-SA1 antibody

Be the first to review this product! Submit a review

|

(24 Publications)

Goat Polyclonal SA1 antibody. Suitable for ICC, IP, ChIP, WB, ChIP/Chip and reacts with Human, Mouse, Xenopus laevis samples. Cited in 24 publications. Immunogen corresponding to Synthetic Peptide within Human STAG1 aa 1150-1200.

View Alternative Names

SA1, SCC3, STAG1, Cohesin subunit SA-1, SCC3 homolog 1, Stromal antigen 1

3 Images
Immunocytochemistry - Anti-SA1 antibody (AB4457)
  • ICC

Unknown

Immunocytochemistry - Anti-SA1 antibody (AB4457)

Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4457 at 1µg/ml.

Immunoprecipitation - Anti-SA1 antibody (AB4457)
  • IP

Supplier Data

Immunoprecipitation - Anti-SA1 antibody (AB4457)

SA1 was immunoprecipitated from HeLa whole cell lysate with 6 μg/mg lysate ab4457. Western blot was performed from the immunoprecipitate using ab4457 at 0.1 μg/ml dilution.

Lane 1 : ab4457 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

All lanes:

Immunoprecipitation - Anti-SA1 antibody (ab4457)

Predicted band size: 144 kDa

false

Western blot - Anti-SA1 antibody (AB4457)
  • WB

Supplier Data

Western blot - Anti-SA1 antibody (AB4457)

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Western blot - Anti-SA1 antibody (ab4457) at 1/10000 dilution

Lane 1:

HeLa Whole cell lysate at 50 µg

Lane 2:

293T Whole cell lysate at 50 µg

Lane 3:

Jurkat Whole cell lysate at 50 µg

Predicted band size: 144 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Xenopus laevis, Mouse

Applications

IP, ICC, ChIP/Chip, WB, ChIP

applications

Immunogen

Synthetic Peptide within Human STAG1 aa 1150-1200. The exact immunogen used to generate this antibody is proprietary information.

Q8WVM7

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ChIPChip" : {"fullname" : "ChIP on chip", "shortname":"ChIP/Chip"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/500 - 1/2000", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ChIPChip-species-checked": "guaranteed", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIPChip-species-checked": "guaranteed", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "<p></p>" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIPChip-species-checked": "predicted", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIPChip-species-checked": "predicted", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIPChip-species-checked": "predicted", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "" }, "Ferret": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIPChip-species-checked": "predicted", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "" }, "Guinea pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIPChip-species-checked": "predicted", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "" }, "Horse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIPChip-species-checked": "predicted", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIPChip-species-checked": "predicted", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "" }, "Rabbit": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIPChip-species-checked": "predicted", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "" }, "Rhesus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIPChip-species-checked": "predicted", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "" }, "Xenopus laevis": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIPChip-species-checked": "guaranteed", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SA1 also known as Stromal Antigen 1 or cohesin subunit SA-1 plays a role in chromosome segregation and sister chromatid cohesion. It has a molecular weight of approximately 130 kDa. SA1 shows expression in various tissues with notable levels in the nucleus of dividing cells. The protein functions as a part of the cohesin complex contributing to its role in regulating the structure and function of chromosomes.
Biological function summary

SA1 works within the cohesin complex alongside other subunits such as SMC1 SMC3 and Rad21. This complex maintains sister chromatid cohesion ensuring proper chromosome alignment and separation during mitosis and meiosis. SA1's interaction within the cohesin complex helps modulate its ability to regulate transcription DNA repair and chromosome organization.

Pathways

SA1 participates in the cohesin pathway which is essential for cell cycle regulation and genomic stability. SA1 interacts with other proteins such as CTCF to facilitate chromosomal loop formation influencing gene expression. Additionally it operates within the DNA damage response pathway aiding in the repair processes by coordinating cohesin-dependent repair mechanisms and maintaining genomic stability.

SA1 has implications in Cornelia de Lange Syndrome (CdLS) and certain cancers. Mutations or dysregulation of SA1 can lead to CdLS a developmental disorder characterized by growth abnormalities and cognitive challenges where Rad21 also plays a role. Moreover altered SA1 expression or function associates with tumorigenesis as its role in chromosome segregation and stability is critical in avoiding aneuploidy and chromosomal instability typical in cancer progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
See full target information STAG1
style
left-column

Publications (24)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107958 PubMed39510176

2024

STAG2 promotes naive-primed transition via activating Lin28a transcription in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Chen,Mingkang Jia,Gan Zhao,Yumin Liu,Yihong Song,Mengjie Sun,Wangfei Chi,Xiangyang Wang,Qing Jiang,Guangwei Xin,Chuanmao Zhang

Cancer discovery 14:1838-1859 PubMed38916500

2024

Biologic and Clinical Analysis of Childhood Gamma Delta T-ALL Identifies LMO2/STAG2 Rearrangements as Extremely High Risk.

Applications

Unspecified application

Species

Unspecified reactive species

Shunsuke Kimura,Chun Shik Park,Lindsey E Montefiori,Ilaria Iacobucci,Petri Pölönen,Qingsong Gao,Elizabeth D Arnold,Andishe Attarbaschi,Anthony Brown,Barbara Buldini,Kenneth J Caldwell,Yunchao Chang,Chelsey Chen,Cheng Cheng,Zhongshan Cheng,John Choi,Valentino Conter,Kristine R Crews,Hester A de Groot-Kruseman,Takao Deguchi,Mariko Eguchi,Hannah E Muhle,Sarah Elitzur,Gabriele Escherich,Burgess B Freeman,Zhaohui Gu,Katie Han,Keizo Horibe,Toshihiko Imamura,Sima Jeha,Motohiro Kato,Kean H Chiew,Tanya Khan,Michal Kicinski,Stefan Köhrer,Steven M Kornblau,Rishi S Kotecha,Chi-Kong Li,Yen-Chun Liu,Franco Locatelli,Selina M Luger,Elisabeth M Paietta,Atsushi Manabe,Hanne V Marquart,Riccardo Masetti,Mellissa Maybury,Pauline Mazilier,Jules P P Meijerink,Sharnise Mitchell,Takako Miyamura,Andrew S Moore,Koichi Oshima,Katarzyna Pawinska-Wasikowska,Rob Pieters,Mollie S Prater,Shondra M Pruett-Miller,Ching-Hon Pui,Chunxu Qu,Michaela Reiterova,Noemi Reyes,Kathryn G Roberts,Jacob M Rowe,Atsushi Sato,Kjeld Schmiegelow,Martin Schrappe,Shuhong Shen,Szymon Skoczeń,Orietta Spinelli,Jan Stary,Michael Svaton,Masatoshi Takagi,Junko Takita,Yanjing Tang,David T Teachey,Paul G Thomas,Daisuke Tomizawa,Jan Trka,Elena Varotto,Tiffaney L Vincent,Jun J Yang,Allen E J Yeoh,Yinmei Zhou,Martin Zimmermann,Hiroto Inaba,Charles G Mullighan

Nucleic acids research 52:5698-5719 PubMed38587186

2024

ARID1A regulates DNA repair through chromatin organization and its deficiency triggers DNA damage-mediated anti-tumor immune response.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Bakr,Giuditta Della Corte,Olivera Veselinov,Simge Kelekçi,Mei-Ju May Chen,Yu-Yu Lin,Gianluca Sigismondo,Marika Iacovone,Alice Cross,Rabail Syed,Yunhee Jeong,Etienne Sollier,Chun-Shan Liu,Pavlo Lutsik,Jeroen Krijgsveld,Dieter Weichenhan,Christoph Plass,Odilia Popanda,Peter Schmezer

Genome biology 24:155 PubMed37381036

2023

RAD21 is the core subunit of the cohesin complex involved in directing genome organization.

Applications

Unspecified application

Species

Unspecified reactive species

Yuao Sun,Xin Xu,Wenxue Zhao,Yu Zhang,Keyang Chen,Yongzheng Li,Xiaotian Wang,Mengling Zhang,Boxin Xue,Wanting Yu,Yingping Hou,Chaobin Wang,Wei Xie,Cheng Li,Daochun Kong,Shu Wang,Yujie Sun

Nature structural & molecular biology 30:853-859 PubMed37081319

2023

Structural basis of centromeric cohesion protection.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto García-Nieto,Amrita Patel,Yan Li,Roel Oldenkamp,Leonardo Feletto,Joshua J Graham,Laureen Willems,Kyle W Muir,Daniel Panne,Benjamin D Rowland

eLife 12: PubMed37010886

2023

Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading.

Applications

Unspecified application

Species

Unspecified reactive species

Hayley Porter,Yang Li,Maria Victoria Neguembor,Manuel Beltran,Wazeer Varsally,Laura Martin,Manuel Tavares Cornejo,Dubravka Pezić,Amandeep Bhamra,Silvia Surinova,Richard G Jenner,Maria Pia Cosma,Suzana Hadjur

Nature communications 13:3218 PubMed35680859

2022

Large-scale multi-omics analysis suggests specific roles for intragenic cohesin in transcriptional regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiankang Wang,Masashige Bando,Katsuhiko Shirahige,Ryuichiro Nakato

Nature communications 13:1859 PubMed35388001

2022

STAG2 regulates interferon signaling in melanoma via enhancer loop reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaowei Chu,Lei Gu,Yeguang Hu,Xiaoyang Zhang,Man Li,Jiajia Chen,Da Teng,Man Huang,Che-Hung Shen,Li Cai,Toshimi Yoshida,Yifeng Qi,Zhixin Niu,Austin Feng,Songmei Geng,Dennie T Frederick,Emma Specht,Adriano Piris,Ryan J Sullivan,Keith T Flaherty,Genevieve M Boland,Katia Georgopoulos,David Liu,Yang Shi,Bin Zheng

Genome biology 23:70 PubMed35241136

2022

Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Eui-Hwan Choi,Seobin Yoon,Young Eun Koh,Tae Kyung Hong,Jeong Tae Do,Bum-Kyu Lee,Yoonsoo Hahn,Keun P Kim

Molecular cell 82:833-851.e11 PubMed35180428

2022

HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Huacheng Luo,Ganqian Zhu,Melanie A Eshelman,Tsz Kan Fung,Qian Lai,Fei Wang,Bernd B Zeisig,Julia Lesperance,Xiaoyan Ma,Shi Chen,Nicholas Cesari,Christopher Cogle,Baoan Chen,Bing Xu,Feng-Chun Yang,Chi Wai Eric So,Yi Qiu,Mingjiang Xu,Suming Huang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB241544

Anti-SA1 antibody [SUSI63B]

Recombinant

Western blot - Anti-SA1 antibody [SUSI63B] (AB241544)

  • 0
  • 0
Primary Antibodies

AB4455

Anti-SA1 antibody

Immunocytochemistry - Anti-SA1 antibody (AB4455)

  • 5
  • 1
Primary Antibodies

AB227736

Anti-SA1 antibody - C-terminal

Western blot - Anti-SA1 antibody - C-terminal (AB227736)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB217678

Anti-Rad21 antibody [EPR22506-15] - ChIP Grade

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Rad21 antibody [EPR22506-15] - ChIP Grade (AB217678)

  • 4
  • 2
Primary Antibodies

AB128919

Anti-SMC3 antibody [EPR7984]

RabMAb|Recombinant

Western blot - Anti-SMC3 antibody [EPR7984] (AB128919)

  • 0
  • 0
Primary Antibodies

AB201451

Anti-SA2 antibody [EPR17865] - C-terminal

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR17865] - C-terminal (AB201451)

  • 5
  • 2
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com