Anti-SA1 antibody [EPR26227-76] (ab317301)

Rabbit Recombinant Monoclonal SA1 antibody. Suitable for WB, Flow Cyt (Intra), IP, ChIP-seq, ICC/IF and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

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Images

Western blot - Anti-SA1 antibody [EPR26227-76] (AB317301), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	Flow Cyt (Intra)	IP	ChIP-seq	ICC/IF	IHC-P
Human	Tested	Tested	Tested	Tested	Tested	Not recommended
Mouse	Tested	Tested	Tested	Expected	Not recommended	Not recommended
Rat	Tested	Expected	Expected	Expected	Expected	Not recommended
Recombinant fragment - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -
Species Mouse	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 8 µg for 10⁷ Cells	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/200	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Target data

See full target information STAG1

Function

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

Alternative names

SA1, SCC3, STAG1, Cohesin subunit SA-1, SCC3 homolog 1, Stromal antigen 1

Recommended products

Rabbit Recombinant Monoclonal SA1 antibody. Suitable for WB, Flow Cyt (Intra), IP, ChIP-seq, ICC/IF and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26227-76
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for mouse ICC.

We recommend using fresh lysate as SA1 can undergo degradation easily. We have observed loss of signal when using frozen lysate.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SA1 also known as Stromal Antigen 1 or cohesin subunit SA-1 plays a role in chromosome segregation and sister chromatid cohesion. It has a molecular weight of approximately 130 kDa. SA1 shows expression in various tissues with notable levels in the nucleus of dividing cells. The protein functions as a part of the cohesin complex contributing to its role in regulating the structure and function of chromosomes.

Biological function summary

SA1 works within the cohesin complex alongside other subunits such as SMC1 SMC3 and Rad21. This complex maintains sister chromatid cohesion ensuring proper chromosome alignment and separation during mitosis and meiosis. SA1's interaction within the cohesin complex helps modulate its ability to regulate transcription DNA repair and chromosome organization.

Pathways

SA1 participates in the cohesin pathway which is essential for cell cycle regulation and genomic stability. SA1 interacts with other proteins such as CTCF to facilitate chromosomal loop formation influencing gene expression. Additionally it operates within the DNA damage response pathway aiding in the repair processes by coordinating cohesin-dependent repair mechanisms and maintaining genomic stability.

Associated diseases and disorders

SA1 has implications in Cornelia de Lange Syndrome (CdLS) and certain cancers. Mutations or dysregulation of SA1 can lead to CdLS a developmental disorder characterized by growth abnormalities and cognitive challenges where Rad21 also plays a role. Moreover altered SA1 expression or function associates with tumorigenesis as its role in chromosome segregation and stability is critical in avoiding aneuploidy and chromosomal instability typical in cancer progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301)
This antibody does not cross-react with human STAG2.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301) at 1/1000 dilution
Lane 1: His-tagged Human STAG1 protein with NFDM/TBST
Lane 2: His-tagged Human STAG2 protein with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 19 kDa, 38 kDa
Exposure time: 180s
Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301)
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with NFDM/TBST
Lane 2: HeLa transfected with siRNA specifically targeting SA1 whole cell lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 145 kDa, 36 kDa
Exposure time: 180s
Immunocytochemistry/ Immunofluorescence - Anti-SA1 antibody [EPR26227-76] (ab317301)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SA1 with ab317301 at 1/200 (10.34 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing mainly nuclear staining in HeLa cell line (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301)
The blot lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
SA1 can undergo degradation easily.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with NFDM/TBST
Lane 3: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 145 kDa, 36 kDa
Exposure time: 180s
Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301)
Lane 1 lysate was freshly made and used for Western Blotting immediately to minimize protein degradation.
SA1 can undergo degradation easily.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: MCF7 whole cell lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 145 kDa, 36 kDa
Exposure time: 180s
ChIP-sequencing - Anti-SA1 antibody [EPR26227-76] (ab317301)
Chromatin was prepared from MCF7 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab317301 [EPR26227-76]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
ChIP-sequencing - Anti-SA1 antibody [EPR26227-76] (ab317301)
Chromatin was prepared from MCF7 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab317301 [EPR26227-76]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
ChIP-sequencing - Anti-SA1 antibody [EPR26227-76] (ab317301)
Chromatin was prepared from MCF7 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab317301 [EPR26227-76]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
Flow Cytometry (Intracellular) - Anti-SA1 antibody [EPR26227-76] (ab317301)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling SA1 with ab317301 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-SA1 antibody [EPR26227-76] (ab317301)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SA1 with ab317301 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-SA1 antibody [EPR26227-76] (ab317301)
SA1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab317301 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317301 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2: ab317301 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317301 in HeLa whole cell lysate
All lanes: Immunoprecipitation - Anti-SA1 antibody [EPR26227-76] (ab317301) at 1/30 dilution
All lanes: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 41s
Immunoprecipitation - Anti-SA1 antibody [EPR26227-76] (ab317301)
SA1 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab317301 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317301 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Lane 2: ab317301 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317301 in NIH/3T3 whole cell lysate
All lanes: Immunoprecipitation - Anti-SA1 antibody [EPR26227-76] (ab317301) at 1/30 dilution
All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 154s