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Rat Recombinant Monoclonal SA1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] (AB241544), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] (AB241544), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] (AB241544), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] (AB241544), expandable thumbnail
  • Western blot - Anti-SA1 antibody [SUSI63B] (AB241544), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
0.153 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
0.153 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
0.153 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
0.765 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
0.765 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
0.765 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

Alternative names

SA1, SCC3, STAG1, Cohesin subunit SA-1, SCC3 homolog 1, Stromal antigen 1

Recommended products

Rat Recombinant Monoclonal SA1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
SUSI63B
Purification technique
Ion exchange chromatography
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SA1 also known as Stromal Antigen 1 or cohesin subunit SA-1 plays a role in chromosome segregation and sister chromatid cohesion. It has a molecular weight of approximately 130 kDa. SA1 shows expression in various tissues with notable levels in the nucleus of dividing cells. The protein functions as a part of the cohesin complex contributing to its role in regulating the structure and function of chromosomes.

Biological function summary

SA1 works within the cohesin complex alongside other subunits such as SMC1 SMC3 and Rad21. This complex maintains sister chromatid cohesion ensuring proper chromosome alignment and separation during mitosis and meiosis. SA1's interaction within the cohesin complex helps modulate its ability to regulate transcription DNA repair and chromosome organization.

Pathways

SA1 participates in the cohesin pathway which is essential for cell cycle regulation and genomic stability. SA1 interacts with other proteins such as CTCF to facilitate chromosomal loop formation influencing gene expression. Additionally it operates within the DNA damage response pathway aiding in the repair processes by coordinating cohesin-dependent repair mechanisms and maintaining genomic stability.

Associated diseases and disorders

SA1 has implications in Cornelia de Lange Syndrome (CdLS) and certain cancers. Mutations or dysregulation of SA1 can lead to CdLS a developmental disorder characterized by growth abnormalities and cognitive challenges where Rad21 also plays a role. Moreover altered SA1 expression or function associates with tumorigenesis as its role in chromosome segregation and stability is critical in avoiding aneuploidy and chromosomal instability typical in cancer progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] (ab241544), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] (ab241544)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling SA1 with ab241544 at 0.765 μg/ml , followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on human colon tissue is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] (ab241544), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] (ab241544)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling SA1 with ab241544 at 0.765 μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on human pancreas tissue is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] (ab241544), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] (ab241544)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling SA1 with ab241544 at 0.765 μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on mouse kidney tissue is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] (ab241544), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] (ab241544)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SA1 with ab241544 at 1/200 dilution, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on rat kidney tissue is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-SA1 antibody [SUSI63B] (ab241544), expandable thumbnail

    Western blot - Anti-SA1 antibody [SUSI63B] (ab241544)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22715410).

    All lanes: Western blot - Anti-SA1 antibody [SUSI63B] (ab241544) at 1/1000 dilution

    Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 10 µg

    Lane 2: MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 10 µg

    Lane 3: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 4: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 5: TF-1 (human Erythroleukemia erythroblast), whole cell lysate at 10 µg

    Lane 6: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

    Lane 7: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution

    Predicted band size: 144 kDa

    Exposure time: 3s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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