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AB4463

Anti-SA2 antibody

5

(1 Review)

|

(11 Publications)

Goat Polyclonal SA2 antibody. Suitable for ICC, IP, WB and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human STAG2 aa 1100-1200.

View Alternative Names

SA2, STAG2, Cohesin subunit SA-2, SCC3 homolog 2, Stromal antigen 2

4 Images
Immunocytochemistry - Anti-SA2 antibody (AB4463)
  • ICC

Unknown

Immunocytochemistry - Anti-SA2 antibody (AB4463)

Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4463 at 1µg/ml.

Immunoprecipitation - Anti-SA2 antibody (AB4463)
  • IP

Supplier Data

Immunoprecipitation - Anti-SA2 antibody (AB4463)

Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Lane 1 : ab4463 used for IP at 6 μg per reaction.

Lane 2 : Control IgG.

For blotting immunoprecipitated SA2, ab4463 was used at 1 μg/ml.

All lanes:

Immunoprecipitation - Anti-SA2 antibody (ab4463)

Predicted band size: 141 kDa

false

Exposure time: 10s

Western blot - Anti-SA2 antibody (AB4463)
  • WB

Supplier Data

Western blot - Anti-SA2 antibody (AB4463)

Whole cell lysate (50 μg) from HeLa, HEK-293T, and NIH/3T3 cells prepared using NETN lysis buffer.

ab4463 used for WB at 0.1 μg/ml.

All lanes:

Western blot - Anti-SA2 antibody (ab4463)

Predicted band size: 141 kDa

false

Exposure time: 10s

Western blot - Anti-SA2 antibody (AB4463)
  • WB

Unknown

Western blot - Anti-SA2 antibody (AB4463)

Nuclear Extract from HeLa cells (~5 x 105 cells).

ab4463 at 0.5 μg/ml.

Detection : ECL with 15 second exposure.

Nuclear Extract from HeLa cells (~5 x 10e5 cells).

ab4463 at 0.5 µg/ml.

Detection : ECL with 15 second exposure.

All lanes:

Western blot - Anti-SA2 antibody (ab4463)

Predicted band size: 141 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP, ICC

applications

Immunogen

Synthetic Peptide within Human STAG2 aa 1100-1200. The exact immunogen used to generate this antibody is proprietary information.

Q8N3U4

Specificity

We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/500 - 1/2000", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SA2 protein also known as stromal antigen 2 is a component of the cohesin complex playing a significant role in chromosomal dynamics. With an approximate molecular weight of 152 kDa SA2 exhibits high expression in the nucleus of proliferating cells particularly during interphase. This cohesin component contributes critically to maintaining sister chromatid cohesion and ensuring proper segregation during cell division serving as a functional partner in the cohesin complex with other components like SMC1 SMC3 and RAD21.
Biological function summary

SA2 plays an important role in regulating gene expression by forming loops that connect different genomic regions. SA2 as a member of the cohesin complex also participates in DNA repair by facilitating accurate recombination enhancing genome stability. This involvement is essential for maintaining chromatin structure which influences diverse cellular processes. Its expression can be an important modulator in the dynamic environment of cellular proliferation and differentiation.

Pathways

SA2 functions integrally within the cohesin-mediated sister chromatid cohesion pathway and the DNA damage response pathway. In these pathways SA2 teams with proteins such as CTCF in insulator function regulation and BRCA1 in DNA repair mechanisms. The cohesin-SA2 interaction with these proteins ensures proper chromosomal architecture and integrity which are essential for the accurate transmission of genetic material across generations of cells.

SA2 mutations or dysregulations have been linked to Cornelia de Lange Syndrome (CdLS) and certain cancers. In CdLS SA2 mutations disrupt normal cohesin function affecting developmental processes. In cancer contexts abnormal SA2 function or expression can lead to improper DNA repair contributing to genomic instability and tumorigenesis. It is often studied in concert with other cohesin proteins and regulatory elements like NIPBL which provides insights into cohesin-related pathologies and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
See full target information STAG2
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 14:1838-1859 PubMed38916500

2024

Biologic and Clinical Analysis of Childhood Gamma Delta T-ALL Identifies LMO2/STAG2 Rearrangements as Extremely High Risk.

Applications

Unspecified application

Species

Unspecified reactive species

Shunsuke Kimura,Chun Shik Park,Lindsey E Montefiori,Ilaria Iacobucci,Petri Pölönen,Qingsong Gao,Elizabeth D Arnold,Andishe Attarbaschi,Anthony Brown,Barbara Buldini,Kenneth J Caldwell,Yunchao Chang,Chelsey Chen,Cheng Cheng,Zhongshan Cheng,John Choi,Valentino Conter,Kristine R Crews,Hester A de Groot-Kruseman,Takao Deguchi,Mariko Eguchi,Hannah E Muhle,Sarah Elitzur,Gabriele Escherich,Burgess B Freeman,Zhaohui Gu,Katie Han,Keizo Horibe,Toshihiko Imamura,Sima Jeha,Motohiro Kato,Kean H Chiew,Tanya Khan,Michal Kicinski,Stefan Köhrer,Steven M Kornblau,Rishi S Kotecha,Chi-Kong Li,Yen-Chun Liu,Franco Locatelli,Selina M Luger,Elisabeth M Paietta,Atsushi Manabe,Hanne V Marquart,Riccardo Masetti,Mellissa Maybury,Pauline Mazilier,Jules P P Meijerink,Sharnise Mitchell,Takako Miyamura,Andrew S Moore,Koichi Oshima,Katarzyna Pawinska-Wasikowska,Rob Pieters,Mollie S Prater,Shondra M Pruett-Miller,Ching-Hon Pui,Chunxu Qu,Michaela Reiterova,Noemi Reyes,Kathryn G Roberts,Jacob M Rowe,Atsushi Sato,Kjeld Schmiegelow,Martin Schrappe,Shuhong Shen,Szymon Skoczeń,Orietta Spinelli,Jan Stary,Michael Svaton,Masatoshi Takagi,Junko Takita,Yanjing Tang,David T Teachey,Paul G Thomas,Daisuke Tomizawa,Jan Trka,Elena Varotto,Tiffaney L Vincent,Jun J Yang,Allen E J Yeoh,Yinmei Zhou,Martin Zimmermann,Hiroto Inaba,Charles G Mullighan

Genome biology 24:155 PubMed37381036

2023

RAD21 is the core subunit of the cohesin complex involved in directing genome organization.

Applications

Unspecified application

Species

Unspecified reactive species

Yuao Sun,Xin Xu,Wenxue Zhao,Yu Zhang,Keyang Chen,Yongzheng Li,Xiaotian Wang,Mengling Zhang,Boxin Xue,Wanting Yu,Yingping Hou,Chaobin Wang,Wei Xie,Cheng Li,Daochun Kong,Shu Wang,Yujie Sun

Cancer cell 39:810-826.e9 PubMed33930311

2021

STAG2 mutations alter CTCF-anchored loop extrusion, reduce cis-regulatory interactions and EWSR1-FLI1 activity in Ewing sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Didier Surdez,Sakina Zaidi,Sandrine Grossetête,Karine Laud-Duval,Anna Sole Ferre,Lieke Mous,Thomas Vourc'h,Franck Tirode,Gaelle Pierron,Virginie Raynal,Sylvain Baulande,Erika Brunet,Véronique Hill,Olivier Delattre

Molecular medicine reports 23: PubMed33537819

2021

CDK6 is stimulated by hyperthermia and protects gastric cancer cells from hyperthermia‑induced damage.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghui Liu,Hongchao Zhao,Qingqing Ding,Haohao Li,Tao Liu,Hongwei Yang,Yuanhua Liu

Molecular cell 73:212-223.e7 PubMed30554942

2018

Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability.

Applications

Unspecified application

Species

Unspecified reactive species

Cornelia Meisenberg,Sarah I Pinder,Suzanna R Hopkins,Sarah K Wooller,Graeme Benstead-Hume,Frances M G Pearl,Penny A Jeggo,Jessica A Downs

The EMBO journal 33:1243-55 PubMed24797475

2014

STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoyuki Fukuda,Nanaho Fukuda,Ana Agostinho,Abrahan Hernández-Hernández,Anna Kouznetsova,Christer Höög

Molecular and cellular biology 31:2174-83 PubMed21444719

2011

Specific sites in the C terminus of CTCF interact with the SA2 subunit of the cohesin complex and are required for cohesin-dependent insulation activity.

Applications

WB

Species

Human

Tiaojiang Xiao,Julie Wallace,Gary Felsenfeld

EMBO reports 12:267-75 PubMed21274006

2011

A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing.

Applications

WB

Species

Mouse

Kei-ichiro Ishiguro,Jihye Kim,Sally Fujiyama-Nakamura,Shigeaki Kato,Yoshinori Watanabe

Genes & development 24:2543-55 PubMed20966046

2010

Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA.

Applications

WB

Species

Human

Hongjie Yao,Kevin Brick,Yvonne Evrard,Tiaojiang Xiao,R Daniel Camerini-Otero,Gary Felsenfeld

Genes & development 24:2169-79 PubMed20889715

2010

Phosphorylation of mammalian Sgo2 by Aurora B recruits PP2A and MCAK to centromeres.

Applications

WB

Species

Human

Yuji Tanno,Tomoya S Kitajima,Takashi Honda,Yasuto Ando,Kei-Ichiro Ishiguro,Yoshinori Watanabe
View all publications
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