Anti-SA2 antibody - C-terminal

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody - C-terminal (AB229609)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SA2 (green) using ab229609 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue). F-actin stained with Phalloidin (red).

• IP

Supplier Data

Immunoprecipitation - Anti-SA2 antibody - C-terminal (AB229609)

SA2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5μg ab229609. Western blot was performed from the immunoprecipitate using ab229609.

Lane 1 : Control IgG instead of ab229609 in HeLa whole cell extract.

Lane 2 : ab229609 IP in HeLa whole cell extract.

All lanes:

Immunoprecipitation - Anti-SA2 antibody - C-terminal (ab229609)

Predicted band size: 141 kDa

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• WB

Supplier Data

Western blot - Anti-SA2 antibody - C-terminal (AB229609)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-SA2 antibody - C-terminal (ab229609) at 1/6000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

SA2 shRNA-transfected HEK-293T whole cell extract at 30 µg

Predicted band size: 141 kDa

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• WB

Supplier Data

Western blot - Anti-SA2 antibody - C-terminal (AB229609)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-SA2 antibody - C-terminal (ab229609) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 141 kDa

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