Rabbit Polyclonal SA2 antibody. C-terminal. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human STAG2.
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- Signal transduction and targeted therapy 7:1922022Dietary folate drives methionine metabolism to promote cancer development by stabilizing MAT IIA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Tao Li,Hai Yang,Ming-Zhu Lei,Wei-Ping Zhu,Ying Su,Kai-Yue Li,Wen-Ying Zhu,Jian Wang,Lei Zhang,Jia Qu,Lei Lv,Hao-Jie Lu,Zheng-Jun Chen,Lu Wang,Miao Yin,Qun-Ying LeiPubMed 35729157
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human STAG2. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N3U4
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/10000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Alternative names
SA2, STAG2, Cohesin subunit SA-2, SCC3 homolog 2, Stromal antigen 2
Recommended products
Rabbit Polyclonal SA2 antibody. C-terminal. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human STAG2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human STAG2. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N3U4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SA2 protein also known as stromal antigen 2 is a component of the cohesin complex playing a significant role in chromosomal dynamics. With an approximate molecular weight of 152 kDa SA2 exhibits high expression in the nucleus of proliferating cells particularly during interphase. This cohesin component contributes critically to maintaining sister chromatid cohesion and ensuring proper segregation during cell division serving as a functional partner in the cohesin complex with other components like SMC1 SMC3 and RAD21.
- Biological function summary
SA2 plays an important role in regulating gene expression by forming loops that connect different genomic regions. SA2 as a member of the cohesin complex also participates in DNA repair by facilitating accurate recombination enhancing genome stability. This involvement is essential for maintaining chromatin structure which influences diverse cellular processes. Its expression can be an important modulator in the dynamic environment of cellular proliferation and differentiation.
- Pathways
SA2 functions integrally within the cohesin-mediated sister chromatid cohesion pathway and the DNA damage response pathway. In these pathways SA2 teams with proteins such as CTCF in insulator function regulation and BRCA1 in DNA repair mechanisms. The cohesin-SA2 interaction with these proteins ensures proper chromosomal architecture and integrity which are essential for the accurate transmission of genetic material across generations of cells.
- Associated diseases and disorders
SA2 mutations or dysregulations have been linked to Cornelia de Lange Syndrome (CdLS) and certain cancers. In CdLS SA2 mutations disrupt normal cohesin function affecting developmental processes. In cancer contexts abnormal SA2 function or expression can lead to improper DNA repair contributing to genomic instability and tumorigenesis. It is often studied in concert with other cohesin proteins and regulatory elements like NIPBL which provides insights into cohesin-related pathologies and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-SA2 antibody - C-terminal (ab229609)
5% SDS-PAGE gel.
All lanes: Western blot - Anti-SA2 antibody - C-terminal (ab229609) at 1/6000 dilution
Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: SA2 shRNA-transfected HEK-293T whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 141 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody - C-terminal (ab229609)
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SA2 (green) using ab229609 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). F-actin stained with Phalloidin (red).
Immunoprecipitation - Anti-SA2 antibody - C-terminal (ab229609)
SA2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5μg ab229609. Western blot was performed from the immunoprecipitate using ab229609.
Lane 1: Control IgG instead of ab229609 in HeLa whole cell extract.
Lane 2: ab229609 IP in HeLa whole cell extract.
All lanes: Immunoprecipitation - Anti-SA2 antibody - C-terminal (ab229609)
Developed using the ECL technique.
Predicted band size: 141 kDa
Western blot - Anti-SA2 antibody - C-terminal (ab229609)
5% SDS-PAGE gel.
All lanes: Western blot - Anti-SA2 antibody - C-terminal (ab229609) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 141 kDa
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