Rabbit Recombinant Monoclonal SA2 antibody. N-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-SA2 antibody [EPR10993] - N-terminal (AB171970), expandable thumbnail](https://content.abcam.com/products/images/sa2-antibody-epr10993-n-terminal-ab171970--western-blot-img7380.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SA2 antibody [EPR10993] - N-terminal (AB171970)")
![Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody [EPR10993] - N-terminal (AB171970), expandable thumbnail](https://content.abcam.com/products/images/sa2-antibody-epr10993-n-terminal-ab171970--immunocytochemistry-immunofluorescence-img127368.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody [EPR10993] - N-terminal (AB171970)")
![Flow Cytometry (Intracellular) - Anti-SA2 antibody [EPR10993] - N-terminal (AB171970), expandable thumbnail](https://content.abcam.com/products/images/sa2-antibody-epr10993-n-terminal-ab171970--flow-cytometry-intracellular-img12852.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SA2 antibody [EPR10993] - N-terminal (AB171970)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Predicted | Predicted |
| Rat | Not recommended | Predicted | Not recommended | Predicted | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Alternative names
SA2, STAG2, Cohesin subunit SA-2, SCC3 homolog 2, Stromal antigen 2
Recommended products
Rabbit Recombinant Monoclonal SA2 antibody. N-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR10993
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SA2 protein also known as stromal antigen 2 is a component of the cohesin complex playing a significant role in chromosomal dynamics. With an approximate molecular weight of 152 kDa SA2 exhibits high expression in the nucleus of proliferating cells particularly during interphase. This cohesin component contributes critically to maintaining sister chromatid cohesion and ensuring proper segregation during cell division serving as a functional partner in the cohesin complex with other components like SMC1 SMC3 and RAD21.
- Biological function summary
SA2 plays an important role in regulating gene expression by forming loops that connect different genomic regions. SA2 as a member of the cohesin complex also participates in DNA repair by facilitating accurate recombination enhancing genome stability. This involvement is essential for maintaining chromatin structure which influences diverse cellular processes. Its expression can be an important modulator in the dynamic environment of cellular proliferation and differentiation.
- Pathways
SA2 functions integrally within the cohesin-mediated sister chromatid cohesion pathway and the DNA damage response pathway. In these pathways SA2 teams with proteins such as CTCF in insulator function regulation and BRCA1 in DNA repair mechanisms. The cohesin-SA2 interaction with these proteins ensures proper chromosomal architecture and integrity which are essential for the accurate transmission of genetic material across generations of cells.
- Associated diseases and disorders
SA2 mutations or dysregulations have been linked to Cornelia de Lange Syndrome (CdLS) and certain cancers. In CdLS SA2 mutations disrupt normal cohesin function affecting developmental processes. In cancer contexts abnormal SA2 function or expression can lead to improper DNA repair contributing to genomic instability and tumorigenesis. It is often studied in concert with other cohesin proteins and regulatory elements like NIPBL which provides insights into cohesin-related pathologies and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-SA2 antibody [EPR10993] - N-terminal (ab171970), expandable thumbnail](https://content.abcam.com/products/images/sa2-antibody-epr10993-n-terminal-ab171970--western-blot-img7380.jpg "Western blot - Anti-SA2 antibody [EPR10993] - N-terminal (ab171970)")
Western blot - Anti-SA2 antibody [EPR10993] - N-terminal (ab171970)
All lanes: Western blot - Anti-SA2 antibody [EPR10993] - N-terminal (ab171970) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: K-562 cell lysate at 10 µg
Lane 3: MCF7 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
SecondaryAll lanes: Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 141 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody [EPR10993] - N-terminal (ab171970), expandable thumbnail](https://content.abcam.com/products/images/sa2-antibody-epr10993-n-terminal-ab171970--immunocytochemistry-immunofluorescence-img127368.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody [EPR10993] - N-terminal (ab171970)")
Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody [EPR10993] - N-terminal (ab171970)
Immunofluorescence analysis of MCF7 cells, labeling SA2 using ab171970 at a 1/100 dilution.
![Flow Cytometry (Intracellular) - Anti-SA2 antibody [EPR10993] - N-terminal (ab171970), expandable thumbnail](https://content.abcam.com/products/images/sa2-antibody-epr10993-n-terminal-ab171970--flow-cytometry-intracellular-img12852.jpg "Flow Cytometry (Intracellular) - Anti-SA2 antibody [EPR10993] - N-terminal (ab171970)")
Flow Cytometry (Intracellular) - Anti-SA2 antibody [EPR10993] - N-terminal (ab171970)
Intracellular flow cytometric analysis of permeabilized Jurkat cells, labeling SA2 using ab171970 at a 1/10 dilution (red) or a rabbit IgG as negative control (green).
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