Rabbit Recombinant Monoclonal SA2 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-SA2 antibody [EPR17865] - BSA and Azide free (AB251341), expandable thumbnail](https://content.abcam.com/products/images/sa2-antibody-epr17865-bsa-and-azide-free-ab251341--western-blot-img5833.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SA2 antibody [EPR17865] - BSA and Azide free (AB251341)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected |
| Mouse | Predicted | Expected | Expected | Expected |
| Rat | Predicted | Expected | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Alternative names
SA2, STAG2, Cohesin subunit SA-2, SCC3 homolog 2, Stromal antigen 2
Recommended products
Rabbit Recombinant Monoclonal SA2 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17865
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251341 is the carrier-free version of Anti-SA2 antibody [EPR17865] - C-terminal ab201451.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SA2 protein also known as stromal antigen 2 is a component of the cohesin complex playing a significant role in chromosomal dynamics. With an approximate molecular weight of 152 kDa SA2 exhibits high expression in the nucleus of proliferating cells particularly during interphase. This cohesin component contributes critically to maintaining sister chromatid cohesion and ensuring proper segregation during cell division serving as a functional partner in the cohesin complex with other components like SMC1 SMC3 and RAD21.
- Biological function summary
SA2 plays an important role in regulating gene expression by forming loops that connect different genomic regions. SA2 as a member of the cohesin complex also participates in DNA repair by facilitating accurate recombination enhancing genome stability. This involvement is essential for maintaining chromatin structure which influences diverse cellular processes. Its expression can be an important modulator in the dynamic environment of cellular proliferation and differentiation.
- Pathways
SA2 functions integrally within the cohesin-mediated sister chromatid cohesion pathway and the DNA damage response pathway. In these pathways SA2 teams with proteins such as CTCF in insulator function regulation and BRCA1 in DNA repair mechanisms. The cohesin-SA2 interaction with these proteins ensures proper chromosomal architecture and integrity which are essential for the accurate transmission of genetic material across generations of cells.
- Associated diseases and disorders
SA2 mutations or dysregulations have been linked to Cornelia de Lange Syndrome (CdLS) and certain cancers. In CdLS SA2 mutations disrupt normal cohesin function affecting developmental processes. In cancer contexts abnormal SA2 function or expression can lead to improper DNA repair contributing to genomic instability and tumorigenesis. It is often studied in concert with other cohesin proteins and regulatory elements like NIPBL which provides insights into cohesin-related pathologies and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-SA2 antibody [EPR17865] - BSA and Azide free (ab251341), expandable thumbnail](https://content.abcam.com/products/images/sa2-antibody-epr17865-bsa-and-azide-free-ab251341--western-blot-img5833.jpg "Western blot - Anti-SA2 antibody [EPR17865] - BSA and Azide free (ab251341)")
Western blot - Anti-SA2 antibody [EPR17865] - BSA and Azide free (ab251341)
This data was developed using the same antibody clone in a different buffer formulation (Anti-SA2 antibody [EPR17865] - C-terminal ab201451).
Lanes 1-3: Merged signal (red and green). Green - Anti-SA2 antibody [EPR17865] - C-terminal ab201451 observed at 141 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-SA2 antibody [EPR17865] - C-terminal ab201451 Anti-SA2 antibody [EPR17865] - C-terminal was shown to specifically react with SA2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human STAG2 (SA2) knockout HeLa cell line ab265461 (knockout cell lysate Human STAG2 (SA2) knockout HeLa cell lysate ab257707) was used. Wild-type and SA2 knockout samples were subjected to SDS-PAGE. Anti-SA2 antibody [EPR17865] - C-terminal ab201451 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SA2 antibody [EPR17865] - C-terminal (Anti-SA2 antibody [EPR17865] - C-terminal ab201451) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: STAG2 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human STAG2 (SA2) knockout HeLa cell line (Human STAG2 (SA2) knockout HeLa cell line ab265461)
Lane 3: HCT116 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 141 kDa
Observed band size: 141 kDa
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