AB199030

Anti-SAA1 + SAA2 antibody [EPR19235]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(21 Publications)

Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) is a rabbit monoclonal antibody detecting SAA1 + SAA2 in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

Serum amyloid A-1 protein, Saa1, Serum amyloid A-2 protein, Saa2

7 Images

Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling SAA1 + SAA2 with ab199030 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/100 dilution (green). Cytoplasmic staining on mouse liver is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SAA1 + SAA2 with ab199030 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse liver is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling SAA1 + SAA2 with ab199030 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on mouse kidney.

Negative control : mouse kidney (PMID : 19747761; PMID : 15623807).

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

SAA1 + SAA2 was immunoprecipitated from 1 mg of mouse serum with ab199030 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199030 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse serum 10 μg (Input).

Lane 2 : ab199030 IP in mouse serum.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199030 in mouse serum.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)

Predicted band size: 13 kDa

false

Supplier Data

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 10 seconds; Lane 2 : 30 seconds.

All lanes:

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/1000 dilution

Lane 1:

Mouse plasma at 10 µg

Lane 2:

Mouse serum at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Supplier Data

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/10000 dilution

Lane 1:

Recombinant mouse SAA1 protein (aa20-122) at 0.01 µg

Lane 2:

GST and His-tagged recombinant mouse SAA2 protein (aa20-122) at 0.005 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Exposure time: 2s

Supplier Data

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/2000 dilution

All lanes:

GST and His-tagged recombinant mouse SAA3 protein (aa1-122) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa

false

Exposure time: 3min

Carrier free

Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19235

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IHC-Fr, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "Antigen retrieval: Heated citric acid solution (10mM citric acid pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse samples.

What is the molecular weight of SAA1 + SAA2?
Anti-SAA1 + SAA2 [EPR19235] (ab199030) specifically detects a band for SAA1 + SAA2 (UniProt: P05366) at a molecular weight of 13kDa.

Trusted by the scientific community
Anti-SAA1 + SAA2 [EPR19235] (ab199030) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR19235] also available for your convenience: ab199030, Carrier free - ab251264

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAA1 and SAA2 collectively known as serum amyloid A proteins belong to the acute-phase reactants family. They play an important role in the body's response to inflammation. Each of these proteins has a molecular mass of approximately 12-14 kDa. They express mainly in hepatic tissue but can also be found in other tissues such as adipose tissue. During an inflammatory response their expression increases significantly making them reliable markers for inflammation.

Biological function summary

These proteins mediate several processes involving immune modulation and cholesterol efflux. SAA1 and SAA2 both function as apolipoproteins primarily associated with high-density lipoprotein (HDL) in plasma. Researchers have identified them as part of a complex of circulating acute-phase proteins that interact with cell surface receptors on various immune cells influencing leukocyte recruitment and adhesion. This interaction facilitates an effective immune response to injury or infection.

Pathways

SAA1 and SAA2 participate significantly in the acute-phase response and cholesterol metabolism pathways. These proteins enhance the transport of cholesterol to the liver for secretion into the bile. Their interaction with proteins like apolipoprotein A-I highlights their role in lipid metabolism. The Toll-like receptor pathways which are vital for innate immunity also see modulation by SAA1 and SAA2 impacting the expression of cytokines involved in inflammatory signaling.

Elevated levels of SAA1 and SAA2 are often linked with chronic inflammatory diseases such as rheumatoid arthritis and cardiovascular diseases. They are involved in the pathogenesis of amyloidosis a condition characterized by the abnormal deposition of amyloid proteins in tissues. Investigations have shown their interaction with amyloid precursor proteins suggesting a pivotal role in the development of amyloid-related pathologies. These connections indicate that SAA1 and SAA2 not only mark disease presence but potentially contribute to its progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Major acute phase protein.

See full target information Serum amyloid A-1 protein

Additional targets

Saa2

style

left-column

Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 10:115 PubMed39557828

2024

Sodium oligomannate disrupts the adherence of Rib bacteria to gut epithelia to block SAA-triggered Th1 inflammation in 5XFAD transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Wang,Zuoquan Xie,Jie Yuan,Enjing Jin,Wen Lian,Shuaishuai Chang,Guangqiang Sun,Zhengnan Feng,Hui Xu,Chen Du,Xinying Yang,Aihua Xia,Ji Qiu,Qingli Zhang,Feifei Lin,Jia Liu,Liang Li,Xiaoguang Du,Zhongping Xiao,Zhou Yi,Zhiyu Luo,Changrong Ge,Rui Li,Mingyue Zheng,Yi Jiang,Tao Wang,Jing Zhang,Qihao Guo,Meiyu Geng

Molecular metabolism 86:101977 PubMed38936659

2024

Liver matrin-3 protects mice against hepatic steatosis and stress response via constitutive androstane receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Cheng,Vijaya Bhaskar Baki,Matthew Moran,Baolong Liu,Jiujiu Yu,Miaoyun Zhao,Qingsheng Li,Jean-Jack Riethoven,Channabasavaiah B Gurumurth,Edward N Harris,Xinghui Sun

Atherosclerosis 391:117492 PubMed38461759

2024

Antisense oligonucleotide targeting hepatic Serum Amyloid A limits the progression of angiotensin II-induced abdominal aortic aneurysm formation.

Applications

Unspecified application

Species

Unspecified reactive species

Preetha Shridas,Ailing Ji,Andrea C Trumbauer,Victoria P Noffsinger,Luke W Meredith,Frederick C de Beer,Adam E Mullick,Nancy R Webb,Dennis G Karounos,Lisa R Tannock

Nature communications 14:7619 PubMed37993434

2023

Mid-old cells are a potential target for anti-aging interventions in the elderly.

Applications

Unspecified application

Species

Unspecified reactive species

Young Hwa Kim,Young-Kyoung Lee,Soon Sang Park,So Hyun Park,So Yeong Eom,Young-Sam Lee,Wonhee John Lee,Juhee Jang,Daeha Seo,Hee Young Kang,Jin Cheol Kim,Su Bin Lim,Gyesoon Yoon,Hong Seok Kim,Jang-Hee Kim,Tae Jun Park

Journal of advanced research 61:223-237 PubMed37717911

2023

Enteric fungi protect against intestinal ischemia-reperfusion injury via inhibiting the SAA1-GSDMD pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Chen,Ben Han,Xu Guan,Guangsheng Du,Baifa Sheng,Xiaoqi Tang,Quanchao Zhang,Huichao Xie,Xianhong Jiang,Qianshan Tan,Shuaishuai Chen,Jian Wang,Wei Chen,Weidong Xiao

Nature communications 14:4960 PubMed37620307

2023

Citrullinated fibrinogen-SAAs complex causes vascular metastagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yibing Han,Takeshi Tomita,Masayoshi Kato,Norihiro Ashihara,Yumiko Higuchi,Hisanori Matoba,Weiyi Wang,Hikaru Hayashi,Yuji Itoh,Satoshi Takahashi,Hiroshi Kurita,Jun Nakayama,Nobuo Okumura,Sachie Hiratsuka

Cell research 33:585-603 PubMed37337030

2023

An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wu,Jiayan Yan,Yinqi Bai,Feiyu Chen,Xuanxuan Zou,Jiangshan Xu,Ao Huang,Liangzhen Hou,Yu Zhong,Zehua Jing,Qichao Yu,Xiaorui Zhou,Zhifeng Jiang,Chunqing Wang,Mengnan Cheng,Yuan Ji,Yingyong Hou,Rongkui Luo,Qinqin Li,Liang Wu,Jianwen Cheng,Pengxiang Wang,Dezhen Guo,Waidong Huang,Junjie Lei,Shang Liu,Yizhen Yan,Yiling Chen,Sha Liao,Yuxiang Li,Haixiang Sun,Na Yao,Xiangyu Zhang,Shiyu Zhang,Xi Chen,Yang Yu,Yao Li,Fengming Liu,Zheng Wang,Shaolai Zhou,Huanming Yang,Shuang Yang,Xun Xu,Longqi Liu,Qiang Gao,Zhaoyou Tang,Xiangdong Wang,Jian Wang,Jia Fan,Shiping Liu,Xinrong Yang,Ao Chen,Jian Zhou

International journal of molecular sciences 24: PubMed37108666

2023

SAA1 Has Potential as a Prognostic Biomarker Correlated with Cell Proliferation, Migration, and an Indicator for Immune Infiltration of Tumor Microenvironment in Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijie Xu,Yunfei Wu,Guanghou Fu,Xiaoyi Chen,Junjie Sun,Junjie Tian,Peng Jiang,Yimin Wang,Baiye Jin

Journal of lipid research 64:100365 PubMed37004910

2023

Serum amyloid A augments the atherogenic effects of cholesteryl ester transfer protein.

Applications

Unspecified application

Species

Unspecified reactive species

Ailing Ji,Andrea C Trumbauer,Victoria P Noffsinger,Frederick C de Beer,Nancy R Webb,Lisa R Tannock,Preetha Shridas

Frontiers in neuroscience 17:1061485 PubMed36968490

2023

Therapeutic effects of CD133 + Exosomes on liver function after stroke in type 2 diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Poornima Venkat,Huanjia Gao,Elizabeth L Findeis,Zhili Chen,Alex Zacharek,Julie Landschoot-Ward,Brianna Powell,Mei Lu,Zhongwu Liu,Zhenggang Zhang,Michael Chopp

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SAA1 + SAA2 antibody [EPR19235]

Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (21)

Product promise