Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) is a rabbit monoclonal antibody that is used to detect SAA1 + SAA2 in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 10 publications
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7239.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18340.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)")
![Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--immunohistochemistry-frozen-sections-img109892.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)")
![Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--western-blot-img45433.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)")
![Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--immunoprecipitation-img10719.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] (AB199030)")
Publications
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- Nature communications 14:76192023Mid-old cells are a potential target for anti-aging interventions in the elderly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoung Hwa Kim,Young-Kyoung Lee,Soon Sang Park,So Hyun Park,So Yeong Eom,Young-Sam Lee,Wonhee John Lee,Juhee Jang,Daeha Seo,Hee Young Kang,Jin Cheol Kim,Su Bin Lim,Gyesoon Yoon,Hong Seok Kim,Jang-Hee Kim,Tae Jun ParkPubMed 37993434
- International journal of molecular sciences 24:2023SAA1 Has Potential as a Prognostic Biomarker Correlated with Cell Proliferation, Migration, and an Indicator for Immune Infiltration of Tumor Microenvironment in Clear Cell Renal Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhijie Xu,Yunfei Wu,Guanghou Fu,Xiaoyi Chen,Junjie Sun,Junjie Tian,Peng Jiang,Yimin Wang,Baiye JinPubMed 37108666
- Journal of lipid research 64:1003652023Serum amyloid A augments the atherogenic effects of cholesteryl ester transfer protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAiling Ji,Andrea C Trumbauer,Victoria P Noffsinger,Frederick C de Beer,Nancy R Webb,Lisa R Tannock,Preetha ShridasPubMed 37004910
- Frontiers in neuroscience 17:10614852023Therapeutic effects of CD133 + Exosomes on liver function after stroke in type 2 diabetic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPoornima Venkat,Huanjia Gao,Elizabeth L Findeis,Zhili Chen,Alex Zacharek,Julie Landschoot-Ward,Brianna Powell,Mei Lu,Zhongwu Liu,Zhenggang Zhang,Michael ChoppPubMed 36968490
- British journal of pharmacology 179:5209-52212022Constitutive androstane receptor (CAR) mediates pyrene-induced inflammatory responses in mouse liver, with increased serum amyloid A proteins and Th17 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhe Shi,Xue Li,Yu-Man Zhang,Yi-Yao Zhou,Xiu-Feng Gan,Qiao-Ying Fan,Chen-Qing He,Tong Shi,Shu-Yun ZhangPubMed 35906855
- PloS one 17:e02666882022Serum Amyloid A is not obligatory for high-fat, high-sucrose, cholesterol-fed diet-induced obesity and its metabolic and inflammatory complications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAiling Ji,Andrea C Trumbauer,Victoria P Noffsinger,Hayce Jeon,Avery C Patrick,Frederick C De Beer,Nancy R Webb,Lisa R Tannock,Preetha ShridasPubMed 35436297
- International journal of molecular sciences 23:2022Regulation of Th17/Treg Balance by 27-Hydroxycholesterol and 24S-Hydroxycholesterol Correlates with Learning and Memory Ability in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Wang,Shanshan Cui,Ling Hao,Wen Liu,Lijing Wang,Mengwei Ju,Wenjing Feng,Rong XiaoPubMed 35457188
- Translational oncology 14:1012282021Transgenic overexpression of the miR-200b/200a/429 cluster inhibits mammary tumor initiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatrina L Watson,Rui Yi,Roger A MooreheadPubMed 34562686
- Neuroscience bulletin 37:1542-15542021Neuroinflammation Mediates Faster Brachial Plexus Regeneration in Subjects with Cerebral Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Su,Guobao Wang,Tie Li,Su Jiang,Aiping Yu,Xiaomin Wang,Wendong XuPubMed 34519993
- Frontiers in immunology 11:5665352020Inhibition of Fatty Acid-Binding Protein 4 Attenuated Kidney Fibrosis by Mediating Macrophage-to-Myofibroblast Transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanhuan Feng et. al.PubMed 33101287
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Mouse | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/40 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Antigen retrieval: Heated citric acid solution (10mM citric acid pH 6.0 + 0.05% Tween-20). |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Product
Additional Targets
Saa2
Alternative names
Serum amyloid A-1 protein, Saa1
Recommended products
Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) is a rabbit monoclonal antibody that is used to detect SAA1 + SAA2 in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 10 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19235
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), in Mouse samples.
What is the molecular weight of SAA1 + SAA2?
Anti-SAA1 + SAA2 [EPR19235] (ab199030) specifically detects a band for SAA1 + SAA2 (UniProt: P05366) at a molecular weight of 13kDa.
Recommended positive controls
WB: Recombinant mouse SAA1 protein (aa20-122); GST and His-tagged recombinant mouse SAA2 protein (aa20-122); Mouse plasma and serum.IHC-P: Mouse liver tissue.IHC-Fr: Mouse liver tissue.IP: Mouse serum.
Trusted by the scientific community
Anti-SAA1 + SAA2 [EPR19235] (ab199030) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products
We have a range of other formats of antibody clone [EPR19235] also available for your convenience:
ab199030, Carrier free - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free ab251264
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SAA1 and SAA2 collectively known as serum amyloid A proteins belong to the acute-phase reactants family. They play an important role in the body's response to inflammation. Each of these proteins has a molecular mass of approximately 12-14 kDa. They express mainly in hepatic tissue but can also be found in other tissues such as adipose tissue. During an inflammatory response their expression increases significantly making them reliable markers for inflammation.
- Biological function summary
These proteins mediate several processes involving immune modulation and cholesterol efflux. SAA1 and SAA2 both function as apolipoproteins primarily associated with high-density lipoprotein (HDL) in plasma. Researchers have identified them as part of a complex of circulating acute-phase proteins that interact with cell surface receptors on various immune cells influencing leukocyte recruitment and adhesion. This interaction facilitates an effective immune response to injury or infection.
- Pathways
SAA1 and SAA2 participate significantly in the acute-phase response and cholesterol metabolism pathways. These proteins enhance the transport of cholesterol to the liver for secretion into the bile. Their interaction with proteins like apolipoprotein A-I highlights their role in lipid metabolism. The Toll-like receptor pathways which are vital for innate immunity also see modulation by SAA1 and SAA2 impacting the expression of cytokines involved in inflammatory signaling.
- Associated diseases and disorders
Elevated levels of SAA1 and SAA2 are often linked with chronic inflammatory diseases such as rheumatoid arthritis and cardiovascular diseases. They are involved in the pathogenesis of amyloidosis a condition characterized by the abnormal deposition of amyloid proteins in tissues. Investigations have shown their interaction with amyloid precursor proteins suggesting a pivotal role in the development of amyloid-related pathologies. These connections indicate that SAA1 and SAA2 not only mark disease presence but potentially contribute to its progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7239.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SAA1 + SAA2 with ab199030 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on mouse liver is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18340.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling SAA1 + SAA2 with ab199030 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative staining on mouse kidney.
Negative control: mouse kidney (PMID:19747761; PMID: 15623807).
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--immunohistochemistry-frozen-sections-img109892.jpg "Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)")
Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling SAA1 + SAA2 with ab199030 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/100 dilution (green). Cytoplasmic staining on mouse liver is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
![Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--western-blot-img45433.jpg "Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)")
Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/10000 dilution
Lane 1: Recombinant mouse SAA1 protein (aa20-122) at 0.01 µg
Lane 2: GST and His-tagged recombinant mouse SAA2 protein (aa20-122) at 0.005 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
Exposure time: 2s
![Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--immunoprecipitation-img10719.jpg "Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)")
Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)
SAA1 + SAA2 was immunoprecipitated from 1 mg of mouse serum with ab199030 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199030 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse serum 10 μg (Input).
Lane 2: ab199030 IP in mouse serum.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199030 in mouse serum.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)
Predicted band size: 13 kDa
![Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--western-blot-img72683.jpg "Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)")
Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/2000 dilution
All lanes: GST and His-tagged recombinant mouse SAA3 protein (aa1-122) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 13 kDa
Exposure time: 3min
![Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--western-blot-img151803.jpg "Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)")
Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 10 seconds; Lane 2: 30 seconds.
All lanes: Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/1000 dilution
Lane 1: Mouse plasma at 10 µg
Lane 2: Mouse serum at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
![Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--western-blot-img72683.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)")
![Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030), expandable thumbnail](https://content.abcam.com/products/images/saa1-saa2-antibody-epr19235-ab199030--western-blot-img151803.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)")
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