AB251264

Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Serum Amyloid A 1 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Serum amyloid A-1 protein, Saa1, Serum amyloid A-2 protein, Saa2

7 Images

Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

This data was developed using ab199030, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling SAA1 + SAA2 with ab199030 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/100 dilution (green). Cytoplasmic staining on mouse liver is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

This data was developed using ab199030, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SAA1 + SAA2 with ab199030 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse liver is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

This data was developed using ab199030, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling SAA1 + SAA2 with ab199030 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on mouse kidney.

Negative control : mouse kidney (PMID : 19747761; PMID : 15623807).

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

This data was developed using ab199030, the same antibody clone in a different buffer formulation.

SAA1 + SAA2 was immunoprecipitated from 1 mg of mouse serum with ab199030 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199030 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse serum 10 μg (Input).
Lane 2 : ab199030 IP in mouse serum.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199030 in mouse serum.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] (<a href='/en-us/products/primary-antibodies/saa1-saa2-antibody-epr19235-ab199030'>ab199030</a>)

Predicted band size: 13 kDa

false

Supplier Data

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

This data was developed using ab199030, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 10 seconds; Lane 2 : 30 seconds.

All lanes:

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (<a href='/en-us/products/primary-antibodies/saa1-saa2-antibody-epr19235-ab199030'>ab199030</a>) at 1/1000 dilution

Lane 1:

Mouse plasma at 10 µg

Lane 2:

Mouse serum at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Supplier Data

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

This data was developed using ab199030, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (<a href='/en-us/products/primary-antibodies/saa1-saa2-antibody-epr19235-ab199030'>ab199030</a>) at 1/10000 dilution

Lane 1:

Recombinant mouse SAA1 protein (aa20-122) at 0.01 µg

Lane 2:

GST and His-tagged recombinant mouse SAA2 protein (aa20-122) at 0.005 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Exposure time: 2s

Supplier Data

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

This data was developed using ab199030, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] (<a href='/en-us/products/primary-antibodies/saa1-saa2-antibody-epr19235-ab199030'>ab199030</a>) at 1/2000 dilution

All lanes:

GST and His-tagged recombinant mouse SAA3 protein (aa1-122) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa

false

Exposure time: 3min

Unconjugated

Anti-SAA1 + SAA2 antibody [EPR19235]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19235

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

WB, IHC-Fr, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Antigen retrieval: Heated citric acid solution (10mM citric acid pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab251264 is the carrier-free version of ab199030.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAA1 and SAA2 collectively known as serum amyloid A proteins belong to the acute-phase reactants family. They play an important role in the body's response to inflammation. Each of these proteins has a molecular mass of approximately 12-14 kDa. They express mainly in hepatic tissue but can also be found in other tissues such as adipose tissue. During an inflammatory response their expression increases significantly making them reliable markers for inflammation.

Biological function summary

These proteins mediate several processes involving immune modulation and cholesterol efflux. SAA1 and SAA2 both function as apolipoproteins primarily associated with high-density lipoprotein (HDL) in plasma. Researchers have identified them as part of a complex of circulating acute-phase proteins that interact with cell surface receptors on various immune cells influencing leukocyte recruitment and adhesion. This interaction facilitates an effective immune response to injury or infection.

Pathways

SAA1 and SAA2 participate significantly in the acute-phase response and cholesterol metabolism pathways. These proteins enhance the transport of cholesterol to the liver for secretion into the bile. Their interaction with proteins like apolipoprotein A-I highlights their role in lipid metabolism. The Toll-like receptor pathways which are vital for innate immunity also see modulation by SAA1 and SAA2 impacting the expression of cytokines involved in inflammatory signaling.

Elevated levels of SAA1 and SAA2 are often linked with chronic inflammatory diseases such as rheumatoid arthritis and cardiovascular diseases. They are involved in the pathogenesis of amyloidosis a condition characterized by the abnormal deposition of amyloid proteins in tissues. Investigations have shown their interaction with amyloid precursor proteins suggesting a pivotal role in the development of amyloid-related pathologies. These connections indicate that SAA1 and SAA2 not only mark disease presence but potentially contribute to its progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major acute phase protein.

See full target information Serum amyloid A-1 protein

Additional targets

Saa2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed38732558

2024

Differential Modulation by Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) of Mesenteric Fat and Macrophages and T Cells in Adipose Tissue of Obese / Zucker Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Hong,Peter Zahradka,Carla G Taylor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free

Immunohistochemistry (Frozen sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

Immunoprecipitation - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

Western blot - Anti-SAA1 + SAA2 antibody [EPR19235] - BSA and Azide free (AB251264)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise