AB251476

Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Serum Amyloid A 1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Recombinant fragment, Human samples. Cited in 1 publication.

View Alternative Names

Serum amyloid A-1 protein, SAA, SAA1, Serum amyloid A-2 protein, SAA2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476)

This data was developed using ab207445, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling SAA1+SAA2 with ab207445 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on human liver cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476)

This data was developed using ab207445, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling SAA1+SAA2 with ab207445 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on human liver is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476)

This data was developed using ab207445, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Expressed by the liver; secreted in plasma and serum (PMID : 3183061; 16039662).

All lanes:

Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] (<a href='/en-us/products/primary-antibodies/saa1-saa2-antibody-epr19550-ab207445'>ab207445</a>) at 1/1000 dilution

All lanes:

Human liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476)

This data was developed using ab207445, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Expressed by the liver; secreted in plasma and serum (PMID : 3183061; 16039662).

All lanes:

Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] (<a href='/en-us/products/primary-antibodies/saa1-saa2-antibody-epr19550-ab207445'>ab207445</a>) at 1/5000 dilution

All lanes:

Human serum at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476)

This data was developed using ab207445, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Human SAA2 and SAA4 fragment recombinant protein contain aa19-122 and aa19-130 with His-Tag^® and GST-tag.

All lanes:

Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] (<a href='/en-us/products/primary-antibodies/saa1-saa2-antibody-epr19550-ab207445'>ab207445</a>) at 1/1000 dilution

Lane 1:

Human SAA1 active recombinant protein at 0.01 µg

Lane 2:

Human SAA2 fragment recombinant protein at 0.01 µg

Lane 3:

Human SAA4 fragment recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa,35 kDa

false

Exposure time: 3min

Unconjugated

Anti-SAA1 + SAA2 antibody [EPR19550]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19550

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251476 is the carrier-free version of ab207445.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAA1 and SAA2 collectively known as serum amyloid A proteins belong to the acute-phase reactants family. They play an important role in the body's response to inflammation. Each of these proteins has a molecular mass of approximately 12-14 kDa. They express mainly in hepatic tissue but can also be found in other tissues such as adipose tissue. During an inflammatory response their expression increases significantly making them reliable markers for inflammation.

Biological function summary

These proteins mediate several processes involving immune modulation and cholesterol efflux. SAA1 and SAA2 both function as apolipoproteins primarily associated with high-density lipoprotein (HDL) in plasma. Researchers have identified them as part of a complex of circulating acute-phase proteins that interact with cell surface receptors on various immune cells influencing leukocyte recruitment and adhesion. This interaction facilitates an effective immune response to injury or infection.

Pathways

SAA1 and SAA2 participate significantly in the acute-phase response and cholesterol metabolism pathways. These proteins enhance the transport of cholesterol to the liver for secretion into the bile. Their interaction with proteins like apolipoprotein A-I highlights their role in lipid metabolism. The Toll-like receptor pathways which are vital for innate immunity also see modulation by SAA1 and SAA2 impacting the expression of cytokines involved in inflammatory signaling.

Elevated levels of SAA1 and SAA2 are often linked with chronic inflammatory diseases such as rheumatoid arthritis and cardiovascular diseases. They are involved in the pathogenesis of amyloidosis a condition characterized by the abnormal deposition of amyloid proteins in tissues. Investigations have shown their interaction with amyloid precursor proteins suggesting a pivotal role in the development of amyloid-related pathologies. These connections indicate that SAA1 and SAA2 not only mark disease presence but potentially contribute to its progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major acute phase protein.

See full target information SAA1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1180 PubMed40775066

2025

Regulation of cortical neurogenesis by MED13L via transcriptional priming and its implications for MED13L syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Li,Yu-Zhu Hao,Jia Cui,Xiao-Xiao Cao,Nan Wu,Ji Ma,Shuang-Yi Xie,Yi-Hsuan Pan,Xue-Lian He,Yu-Lan Zhao,Xiao-Bing Yuan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476)

Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476)

Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476)

Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise