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Rabbit Recombinant Monoclonal Serum Amyloid A 1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Recombinant fragment, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476), expandable thumbnail
  • Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476), expandable thumbnail
  • Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476), expandable thumbnail
  • Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (AB251476), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Recombinant fragment
Tested
Tested

Tested
Tested

Species
Recombinant fragment
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Recombinant fragment
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Major acute phase protein.

Additional Targets

SAA2

Alternative names

Serum amyloid A-1 protein, SAA, SAA1

Recommended products

Rabbit Recombinant Monoclonal Serum Amyloid A 1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Recombinant fragment, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR19550
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251476 is the carrier-free version of Anti-SAA1 + SAA2 antibody [EPR19550] ab207445.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SAA1 and SAA2 collectively known as serum amyloid A proteins belong to the acute-phase reactants family. They play an important role in the body's response to inflammation. Each of these proteins has a molecular mass of approximately 12-14 kDa. They express mainly in hepatic tissue but can also be found in other tissues such as adipose tissue. During an inflammatory response their expression increases significantly making them reliable markers for inflammation.

Biological function summary

These proteins mediate several processes involving immune modulation and cholesterol efflux. SAA1 and SAA2 both function as apolipoproteins primarily associated with high-density lipoprotein (HDL) in plasma. Researchers have identified them as part of a complex of circulating acute-phase proteins that interact with cell surface receptors on various immune cells influencing leukocyte recruitment and adhesion. This interaction facilitates an effective immune response to injury or infection.

Pathways

SAA1 and SAA2 participate significantly in the acute-phase response and cholesterol metabolism pathways. These proteins enhance the transport of cholesterol to the liver for secretion into the bile. Their interaction with proteins like apolipoprotein A-I highlights their role in lipid metabolism. The Toll-like receptor pathways which are vital for innate immunity also see modulation by SAA1 and SAA2 impacting the expression of cytokines involved in inflammatory signaling.

Associated diseases and disorders

Elevated levels of SAA1 and SAA2 are often linked with chronic inflammatory diseases such as rheumatoid arthritis and cardiovascular diseases. They are involved in the pathogenesis of amyloidosis a condition characterized by the abnormal deposition of amyloid proteins in tissues. Investigations have shown their interaction with amyloid precursor proteins suggesting a pivotal role in the development of amyloid-related pathologies. These connections indicate that SAA1 and SAA2 not only mark disease presence but potentially contribute to its progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)

    This data was developed using Anti-SAA1 + SAA2 antibody [EPR19550] ab207445, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human liver tissue labeling SAA1+SAA2 with Anti-SAA1 + SAA2 antibody [EPR19550] ab207445 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476), expandable thumbnail

    Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)

    This data was developed using Anti-SAA1 + SAA2 antibody [EPR19550] ab207445, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human SAA2 and SAA4 fragment recombinant protein contain aa19-122 and aa19-130 with His-Tag® and GST-tag.

    All lanes: Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] (Anti-SAA1 + SAA2 antibody [EPR19550] ab207445) at 1/1000 dilution

    Lane 1: Human SAA1 active recombinant protein at 0.01 µg

    Lane 2: Human SAA2 fragment recombinant protein at 0.01 µg

    Lane 3: Human SAA4 fragment recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa

    Observed band size: 13 kDa, 35 kDa

    Exposure time: 3min

  • Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476), expandable thumbnail

    Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)

    This data was developed using Anti-SAA1 + SAA2 antibody [EPR19550] ab207445, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Expressed by the liver; secreted in plasma and serum (PMID: 3183061; 16039662).

    All lanes: Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] (Anti-SAA1 + SAA2 antibody [EPR19550] ab207445) at 1/5000 dilution

    All lanes: Human serum at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 13 kDa

    Observed band size: 13 kDa

    Exposure time: 3min

  • Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476), expandable thumbnail

    Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)

    This data was developed using Anti-SAA1 + SAA2 antibody [EPR19550] ab207445, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Expressed by the liver; secreted in plasma and serum (PMID: 3183061; 16039662).

    All lanes: Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] (Anti-SAA1 + SAA2 antibody [EPR19550] ab207445) at 1/1000 dilution

    All lanes: Human liver lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 13 kDa

    Observed band size: 13 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)

    This data was developed using Anti-SAA1 + SAA2 antibody [EPR19550] ab207445, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling SAA1+SAA2 with Anti-SAA1 + SAA2 antibody [EPR19550] ab207445 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on human liver cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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