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AB231680

Anti-SAA3 antibody [JOR110A]

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(9 Publications)

Anti-SAA3 antibody [JOR110A] (ab231680) is a rat monoclonal antibody detecting SAA3 in Western Blot, IHC-P. Suitable for Mouse.

View Alternative Names

Serum amyloid A-3 protein, Saa3

14 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)

Paraffin-embedded mouse colitis tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

Note this image was from a different batch produced using hybridoma method

Immunocytochemistry - Anti-SAA3 antibody [JOR110A] (AB231680)
  • ICC

Supplier Data

Immunocytochemistry - Anti-SAA3 antibody [JOR110A] (AB231680)

Frozen cytospin preparation of MYC-tagged SAA4-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) stained for SAA3 using ab231680 at 1/50 dilution in immunocytochemical analysis (negative control).

Note this image was from a different batch produced using hybridoma method

Immunocytochemistry - Anti-SAA3 antibody [JOR110A] (AB231680)
  • ICC

Supplier Data

Immunocytochemistry - Anti-SAA3 antibody [JOR110A] (AB231680)

Frozen MYC-tagged SAA3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cytospin stained for SAA3 using ab231680 at 1/50 dilution in immunocytochemical analysis.

Note this image was from a different batch produced using hybridoma method

Immunocytochemistry - Anti-SAA3 antibody [JOR110A] (AB231680)
  • ICC

Supplier Data

Immunocytochemistry - Anti-SAA3 antibody [JOR110A] (AB231680)

Frozen cytospin preparation of MYC-tagged SAA2-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) stained for SAA3 using ab231680 at 1/50 dilution in immunocytochemical analysis (negative control).

Note this image was from a different batch produced using hybridoma method

Immunocytochemistry - Anti-SAA3 antibody [JOR110A] (AB231680)
  • ICC

Supplier Data

Immunocytochemistry - Anti-SAA3 antibody [JOR110A] (AB231680)

Frozen cytospin preparation of MYC-tagged SAA1-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) stained for SAA3 using ab231680 at 1/50 dilution in immunocytochemical analysis.

Note this image was from a different batch produced using hybridoma method

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)

Paraffin-embedded mouse pneumonia tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

Note this image was from a different batch produced using hybridoma method

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)

Paraffin-embedded mouse colon tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

Note this image was from a different batch produced using hybridoma method

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)

Paraffin-embedded mouse adepocytes in SAA KO mouse stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

Note this image was from a different batch produced using hybridoma method

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)

Paraffin-embedded mouse mesothelium tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

Note this image was from a different batch produced using hybridoma method

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)

Paraffin-embedded mouse adipocytes in adenocarcinoma tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

Note this image was from a different batch produced using hybridoma method

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)

Paraffin-embedded mouse adenocarcinoma tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

Note this image was from a different batch produced using hybridoma method

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (AB231680)

Paraffin-embedded mouse lung tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

Note this image was from a different batch produced using hybridoma method

Western blot - Anti-SAA3 antibody [JOR110A] (AB231680)
  • WB

Supplier Data

Western blot - Anti-SAA3 antibody [JOR110A] (AB231680)

Note this image was from a different batch produced using hybridoma method

All lanes:

Western blot - Anti-SAA3 antibody [JOR110A] (ab231680) at 1/50 dilution

Lane 1:

SAA1-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 2:

SAA2-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 3:

SAA3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 4:

SAA4-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 5:

RAW12 supernatant at 20 µL

Lane 6:

RAW12 stimulated with LPS for 12 hours supernatant at 20 µL

Predicted band size: 14 kDa

true

Western blot - Anti-SAA3 antibody [JOR110A] (AB231680)
  • WB

Supplier Data

Western blot - Anti-SAA3 antibody [JOR110A] (AB231680)

Note this image was from a different batch produced using hybridoma method

All lanes:

Western blot - Anti-SAA3 antibody [JOR110A] (ab231680) at 1/50 dilution

Lane 1:

RAW12 stimulated with LPS for 8 hours supernatant at 20 µL

Lane 2:

RAW12 stimulated with LPS for 12 hours supernatant at 20 µL

Lane 3:

RAW12 stimulated with LPS for 16 hours supernatant at 20 µL

Lane 4:

RAW12 stimulated with LPS for 24 hours supernatant at 20 µL

Lane 5:

RAW12 supernatant at 20 µL

Lane 6:

Mouse SAA3-GFP-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Predicted band size: 14 kDa

true

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

JOR110A

Isotype

IgG2b

Carrier free

No

Reacts with

Mouse

Applications

ICC, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Saa3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P04918

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Transfected cell line - Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/50", "ICC-species-notes": "<p></p>" }, "Transfected cell lysate - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-SAA3 antibody [JOR110A] (ab231680) is a rat monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Mouse samples.

What is the molecular weight of SAA3?
Anti-SAA3 [JOR110A] (ab231680) specifically detects a band for SAA3 (UniProt: P04918) at a molecular weight of 14kDa.

The production method has been switched from hybridoma to recombinant on 24th Oct 2025.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
-20°C|+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid A3 (SAA3) is a member of the serum amyloid A family of proteins and is known for its acute-phase response activity. This protein has an approximate mass of 14 kDa. SAA3 expression is mainly found in extrahepatic tissues in response to pro-inflammatory stimuli. It increases quickly during inflammation and tissue injury serving as a sensitive marker for inflammatory diseases. SAA3's function in the immune response makes it an important protein for researchers studying inflammation and related conditions.
Biological function summary

SAA3 modulates inflammatory responses and may play a role in the pathogenesis of chronic inflammatory diseases. SAA3 proteins can associate with high-density lipoprotein (HDL) in the bloodstream. Such association changes the lipid metabolism and cholesterol transport affecting the body's inflammatory state. The protein also participates in attracting immune cells like leukocytes to the site of inflammation contributing to immune surveillance and tissue repair.

Pathways

Several studies show that SAA3 participates in the toll-like receptor (TLR) signaling and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) pathways. These pathways are key regulators of the immune response. SAA3 activation can lead to increased cytokine production such as interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). Its interactions with other SAAs suggest an overlap in their immune-modulating roles making them important players in systemic inflammation.

SAA3 is linked to conditions like chronic inflammatory diseases and cancer. In rheumatoid arthritis for instance elevated levels of SAA3 correlate with disease severity implicating it as a potential biomarker. The protein is also associated with atherosclerosis where it may influence the development of arterial plaques. Its connection to SAA1 and SAA2 which are also related to inflammation and amyloid deposition highlights a broader role for SAAs in inflammatory and amyloid-related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major acute phase reactant. Apolipoprotein of the HDL complex. In vitro exhibits antimicrobial activity against Escherichia coli, Streptococcus uberis and Pseudomonas aeruginosa (By similarity).
See full target information Saa3
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:284 PubMed39987372

2025

Emergence of inflammatory fibroblasts with aging in Hermansky-Pudlak syndrome associated pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon J H Banaschewski,Sylvia N Michki,Sneha Sitaraman,Ruby Pan,Joanna Y Wang,Dominique Stewart,Mary Kate Goldy,Susan M Lin,Edward Cantu,Jeremy B Katzen,Maria C Basil,Amir M Emtiazjoo,Jamie L Todd,Jason J Gokey,Jonathan A Kropski,David B Frank,Jarod A Zepp,Lisa R Young

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23389 PubMed38153347

2023

Serum amyloid A aggravates endotoxin-induced ocular inflammation through the regulation of retinal microglial activation.

Applications

Unspecified application

Species

Unspecified reactive species

Huazhang Feng,Wenchuan Zhou,Yuan Yang,Xuerui Zhang,Ruixue Mao,Yutong Zhou,Tongjie Cheng,Haodong Xiao,Yuqing Rao,Jincan He,Peiquan Zhao,Jing Li,Chunhui Jiang

Bone & joint research 12:615-623 PubMed37783468

2023

Regeneration of injured articular cartilage using the recombinant human amelogenin protein.

Applications

Unspecified application

Species

Unspecified reactive species

Omer Helwa-Shalom,Faris Saba,Elad Spitzer,Salem Hanhan,Koby Goren,Shany I Markowitz,Dekel Shilo,Nissim Khaimov,Yechiel N Gellman,Dan Deutsch,Anat Blumenfeld,Hani Nevo,Amir Haze

Annals of translational medicine 10:1332 PubMed36660634

2023

Transcriptome sequencing analysis of the role of β-catenin in F-actin reorganization in embryonic palatal mesenchymal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Weilong Liu,Yong Lu,Bing Shi,Chenghao Li

JCI insight 7: PubMed36134659

2022

A gut-oral microbiome-driven axis controls oropharyngeal candidiasis through retinoic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Ey Aggor,Martinna Bertolini,Chunsheng Zhou,Tiffany C Taylor,Darryl A Abbott,Javonn Musgrove,Vincent M Bruno,Timothy W Hand,Sarah L Gaffen

Analytical cellular pathology (Amsterdam) 2022:7847135 PubMed35132370

2022

Effect of Polysaccharide on Decreasing Serum Amyloid A3 Expression through Inhibiting NF-B Activation in a Mouse Model of Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Fengqi Wan,Fulin Ma,Jiaxin Wu,Xinyu Qiao,Minxue Chen,Wenjian Li,Liang Ma

Animals : an open access journal from MDPI 11: PubMed34070499

2021

Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid.

Applications

Unspecified application

Species

Unspecified reactive species

Sato Kamiya,Kaori Shimizu,Ayaka Okada,Yasuo Inoshima

Cell 180:79-91.e16 PubMed31866067

2019

Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.

Applications

Unspecified application

Species

Unspecified reactive species

June-Yong Lee,Jason A Hall,Lina Kroehling,Lin Wu,Tariq Najar,Henry H Nguyen,Woan-Yu Lin,Stephen T Yeung,Hernandez Moura Silva,Dayi Li,Ashley Hine,P'ng Loke,David Hudesman,Jerome C Martin,Ephraim Kenigsberg,Miriam Merad,Kamal M Khanna,Dan R Littman

Experimental cell research 383:111454 PubMed31170401

2019

Exosomes derived from microRNA-30b-3p-overexpressing mesenchymal stem cells protect against lipopolysaccharide-induced acute lung injury by inhibiting SAA3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Yi,Xuxia Wei,Haijin Lv,Yuling An,Lijuan Li,Pinglan Lu,Yang Yang,Qi Zhang,Huimin Yi,Guihua Chen
View all publications
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Product promise

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