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AB122809

Anti-SAC3D1 antibody

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(2 Publications)

Rabbit Polyclonal SAC3D1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SAC3D1 aa 250-400.

View Alternative Names

SHD1, SAC3D1, SAC3 domain-containing protein 1, SAC3 homology domain-containing protein 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAC3D1 antibody (AB122809)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAC3D1 antibody (AB122809)

ab122809, at 1/200 dilution, staining SAC3D1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-SAC3D1 antibody (AB122809)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SAC3D1 antibody (AB122809)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122809 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SAC3D1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

A6NKF1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAC3D1 also known as SH3- and SAC domain-containing protein 1 is a protein that plays a mechanical role in intracellular transport and phosphoinositide metabolism. The protein has a mass of approximately 94 kDa and is expressed in various tissues with higher levels in the brain and testis. SAC3D1 contains an SH3 domain and a phosphatase domain which are important for its function in cellular processes.
Biological function summary

SAC3D1 interacts with other proteins to form a complex critical for phosphatidylinositol-3-phosphate binding and turnover. This activity is essential in membrane trafficking regulating endosomal dynamics and impacting cellular signaling pathways. SAC3D1's ability to mediate phosphoinositide signaling suggests that it plays a role in cell growth and differentiation maintaining normal cellular functions.

Pathways

SAC3D1 is involved in the phosphoinositide signaling pathway and the endocytic recycling pathway. These pathways include interactions with proteins like MTMR2 and FIG4 which are important for lipid phosphatase activity and vesicle recycling. SAC3D1's association with these proteins highlights its influence on maintaining endosome homeostasis within cells important for proper signaling and trafficking functions.

SAC3D1 has been linked to conditions such as Charcot-Marie-Tooth disease and Amyotrophic Lateral Sclerosis (ALS). Its interactions with the FIG4 protein is significant as mutations in FIG4 relate to these disorders. Disruptions in SAC3D1 function can lead to defects in lipid metabolism and neuronal maintenance contributing to the pathogenesis of these neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in centrosome duplication and mitotic progression.
See full target information SAC3D1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cytogenetic and genome research 161:167-177 PubMed33951625

2021

Mus musculus Barrier-To-Autointegration Factor 2 (Banf2) is Not Essential for Spermatogenesis or Fertility.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Min Niu,Meng-Meng Xia,Ya-Nan Zhong,Ying Zheng

International journal of oncology 57:122-138 PubMed32319600

2020

Upregulated expression of SAC3D1 is associated with progression in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

An-Gui Liu,Jin-Cai Zhong,Gang Chen,Rong-Quan He,Yi-Qiang He,Jie Ma,Li-Hua Yang,Xiao-Jv Wu,Jun-Tao Huang,Jian-Jun Li,Wei-Jia Mo,Xin-Gan Qin
View all publications
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