AB230642

Anti-SACM1L/Sac1 antibody

Name: Anti-SACM1L/Sac1 antibody (ab230642) | Abcam
Brand: Abcam Limited
SKU: AB230642
Price: 605 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SACM1L/Sac1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SACM1L aa 400-450 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA0851, SAC1, SACM1L, Phosphatidylinositol-3-phosphatase SAC1, Phosphatidylinositol-4-phosphate phosphatase, Suppressor of actin mutations 1-like protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SACM1L/Sac1 antibody (AB230642)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SACM1L/Sac1 antibody (AB230642)

Formalin-fixed, paraffin-embedded human kidney tissue stained for SACM1L/Sac1 with ab230642 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SACM1L/Sac1 antibody (AB230642)

Formalin-fixed, paraffin-embedded human colon tissue stained for SACM1L/Sac1 with ab230642 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-SACM1L/Sac1 antibody (AB230642)

All lanes:

Anti-CSRP2BP antibody (ab230641) at 1/1000 dilution

All lanes:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 35 µg

Predicted band size: 67 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SACM1L aa 400-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9NTJ5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SACM1L also known as Sac1 is an integral enzyme in the cell performing its role as a phosphoinositide phosphatase. It has a mass of approximately 59 kDa. SACM1L localizes in the cytoplasm and the membrane systems of the endoplasmic reticulum and Golgi apparatus where it performs its enzymatic activities. Its expression is observed in many tissues with particular abundance in the brain and testis. The protein's ability to dephosphorylate phosphoinositides highlights its mechanical role in cellular lipid metabolism.

Biological function summary

This enzyme regulates intracellular lipid signaling and trafficking by modulating the levels of phosphoinositides. SACM1L is part of a protein complex involving other regulatory proteins that maintain the balance of lipid phosphates. Through its enzymatic activity SACM1L influences membrane dynamics and vesicular transport within cells. Taken together these activities show its importance in maintaining cellular homeostasis and function.

Pathways

SACM1L plays a role in phosphatidylinositol signaling and the vesicle-mediated transport pathway. Its action in these pathways involves interaction with proteins such as PIKfyve and Synaptojanin which are present in similar signaling events. SACM1L ensures the proper turnover of phosphatidylinositides which is critical for membrane identity and function in transport processes. These interactions highlight SACM1L's relevance to key signaling cascades within the cell.

SACM1L is implicated in neurodegenerative diseases and congenital disorders of glycosylation. Mutations or dysregulation in SACM1L have been linked to certain types of hereditary spastic paraplegia where it influences neuronal function and integrity. Additionally SACM1L's connection with proteins such as PGP and other phosphatases suggests its involvement in maintaining glycosylation patterns and cellular stress responses. Understanding its connection to these diseases could provide insights into novel therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphoinositide phosphatase which catalyzes the hydrolysis of phosphatidylinositol 4-phosphate (PtdIns(4)P) (PubMed : 24209621, PubMed : 27044890, PubMed : 29461204, PubMed : 30659099). Can also catalyze the hydrolysis of phosphatidylinositol 3-phosphate (PtdIns(3)P) and has low activity towards phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2) (By similarity). Shows a very robust PtdIns(4)P phosphatase activity when it binds PtdIns(4)P in a 'cis' configuration in the cellular environment, with much less activity seen when it binds PtdIns(4)P in 'trans' configuration (PubMed : 24209621, PubMed : 29461204, PubMed : 30659099). PtdIns(4)P phosphatase activity (when it binds PtdIns(4)P in 'trans' configuration) is enhanced in the presence of PLEKHA3 (PubMed : 30659099).

See full target information SACM1L

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 61:1004-1013 PubMed32371566

2020

Mass spectrometry imaging and LC/MS reveal decreased cerebellar phosphoinositides in Niemann-Pick type C1-null mice.

Applications

Unspecified application

Species

Unspecified reactive species

Koralege C Pathmasiri,Melissa R Pergande,Fernando Tobias,Rima Rebiai,Avia Rosenhouse-Dantsker,Ernesto R Bongarzone,Stephanie M Cologna

Evidence-based complementary and alternative medic 2020:7061972 PubMed32104194

2020

Electroacupuncture Alleviates Experimental Chronic Inflammatory Pain by Inhibiting Calcium Voltage-Gated Channel-Mediated Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhou,Ying Jin,Ruijie Ma,Hongyun Song,Qin Chen,Yueyang Chai,Yi Liang,You Zhou,Jianqiao Fang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SACM1L/Sac1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SACM1L/Sac1 antibody (AB230642)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SACM1L/Sac1 antibody (AB230642)

Western blot - Anti-SACM1L/Sac1 antibody (AB230642)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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