Rabbit Recombinant Monoclonal Sacsin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (AB250426), expandable thumbnail](https://content.abcam.com/products/images/sacsin-antibody-epr11864-61-bsa-and-azide-free-ab250426--flow-cytometry-intracellular-img2419.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (AB250426)")
![Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (AB250426), expandable thumbnail](https://content.abcam.com/products/images/sacsin-antibody-epr11864-61-bsa-and-azide-free-ab250426--western-blot-img10321.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (AB250426)")
![Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (AB250426), expandable thumbnail](https://content.abcam.com/products/images/sacsin-antibody-epr11864-61-bsa-and-azide-free-ab250426--western-blot-img4900.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (AB250426)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Co-chaperone which acts as a regulator of the Hsp70 chaperone machinery and may be involved in the processing of other ataxia-linked proteins.
Alternative names
DNAJC29, KIAA0730, SACS, Sacsin, DnaJ homolog subfamily C member 29
Recommended products
Rabbit Recombinant Monoclonal Sacsin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11864-61
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250426 is the carrier-free version of Anti-Sacsin antibody [EPR11864-61] ab181207.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry (Intracellular) - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426), expandable thumbnail](https://content.abcam.com/products/images/sacsin-antibody-epr11864-61-bsa-and-azide-free-ab250426--flow-cytometry-intracellular-img2419.jpg "Flow Cytometry (Intracellular) - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)")
Flow Cytometry (Intracellular) - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)
This data was developed using Anti-Sacsin antibody [EPR11864-61] ab181207, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling Sacsin with Anti-Sacsin antibody [EPR11864-61] ab181207 at 1/470 dilution (red), or a rabbit IgG isotype control (green). Goat anti rabbit IgG (FITC) was used as a secondary antibody at 1/150 dilution.
![Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426), expandable thumbnail](https://content.abcam.com/products/images/sacsin-antibody-epr11864-61-bsa-and-azide-free-ab250426--western-blot-img10321.jpg "Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)")
Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)
This data was developed using Anti-Sacsin antibody [EPR11864-61] ab181207, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Sacsin antibody [EPR11864-61] (Anti-Sacsin antibody [EPR11864-61] ab181207) at 1/5000 dilution
Lane 1: Human fetal brain lysate at 20 µg
Lane 2: Human glioma cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 521 kDa
![Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426), expandable thumbnail](https://content.abcam.com/products/images/sacsin-antibody-epr11864-61-bsa-and-azide-free-ab250426--western-blot-img4900.jpg "Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)")
Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)
This data was developed using Anti-Sacsin antibody [EPR11864-61] ab181207, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Sacsin antibody [EPR11864-61] (Anti-Sacsin antibody [EPR11864-61] ab181207) at 1/20000 dilution
All lanes: HeLa cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 521 kDa
Downloads
Product protocols
- Visit the general protocols
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