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AB185949

Anti-SAE1 antibody [EPR15397(B)]

3

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal SAE1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 7 publications.

View Alternative Names

AOS1, SUA1, UBLE1A, SAE1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15397(B)] (AB185949)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15397(B)] (AB185949)

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling SAE1 with ab185949 at 1/100 dilution (red). Secondary antibody is a Goat anti-rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counterstain : DAPI (blue).

Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15397(B)] (AB185949)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15397(B)] (AB185949)

Immunofluorescent analysis of -20℃ acetone-fixed HeLa cells labeling SAE1 with ab185949 at 1/100 dilution. Secondary ab : Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counter stain : Dapi (blue).

Western blot - Anti-SAE1 antibody [EPR15397(B)] (AB185949)
  • WB

Supplier Data

Western blot - Anti-SAE1 antibody [EPR15397(B)] (AB185949)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/1000 dilution

Lane 1:

Hela cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

Western blot - Anti-SAE1 antibody [EPR15397(B)] (AB185949)
  • WB

Supplier Data

Western blot - Anti-SAE1 antibody [EPR15397(B)] (AB185949)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/10000 dilution

All lanes:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

Western blot - Anti-SAE1 antibody [EPR15397(B)] (AB185949)
  • WB

Supplier Data

Western blot - Anti-SAE1 antibody [EPR15397(B)] (AB185949)

All lanes:

Western blot - Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/1000 dilution

Lane 1:

Raw264.7 cell lysate at 10 µg

Lane 2:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

  • Carrier free

    Anti-SAE1 antibody [EPR15397(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15397(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein SAE1 also known as SUMO-activating enzyme subunit 1 functions mechanically as a part of the SUMOylation process. It has a mass of approximately 39 kDa and is expressed in multiple cell types but is mainly found in the nucleus. SAE1 forms a heterodimeric structure with the protein SAE2 and together they are responsible for the initial step of SUMO activation which is important for SUMOylation a post-translational modification process.
Biological function summary

SAE1 is integral to numerous cellular processes that require precise regulation such as transcription DNA repair and signal transduction. It functions as part of the SUMO E1 enzyme complex which is essential for conjugating SUMO proteins to target substrates. This modification influences protein stability localization and interaction adding a layer of control in these cellular activities. The modulation of protein function by SAE1 impacts cell cycle control and stress responses indicating its breadth of influence across cellular mechanisms.

Pathways

The actions of SAE1 significantly impact the PI3K-Akt and DNA damage repair pathways. Its involvement in these pathways highlights its role in regulating cell survival and maintaining genomic integrity. SAE1's interaction within the SUMOylation machinery connects it to other proteins such as Ubc9 the E2 conjugating enzyme which highlights its importance in facilitating modifications necessary for a wide array of cellular functions and signaling cascades.

Defects or dysregulation of SAE1 have been linked to cancer and neurodegenerative conditions. Altered SUMOylation levels controlled by SAE1 can influence tumorigenesis through abnormal cell proliferation or impaired DNA damage responses connecting it with proteins like p53 and PARP1 which are critical in those pathways. Additionally SAE1's involvement in neurological disorders is associated with disrupted protein homeostasis linking it to pathologies where proper cellular regulatory mechanisms are compromised.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
See full target information SAE1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7248 PubMed40769964

2025

IRX3 controls a SUMOylation-dependent differentiation switch in adipocyte precursor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jan-Inge Bjune,Samantha Laber,Laurence Lawrence-Archer,Patrizia M C Nothnagel,Shuntaro Yamada,Xu Zhao,Pouda Panahandeh Strømland,Niyaz Al-Sharabi,Kamal Mustafa,Pål R Njølstad,Melina Claussnitzer,Roger D Cox,Pierre Chymkowitch,Gunnar Mellgren,Simon N Dankel

Cell death & disease 15:674 PubMed39277582

2024

Impaired SUMOylation of FoxA1 promotes nonalcoholic fatty liver disease through down-regulation of Sirt6.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Zou,Jinwen Liao,Min Xiao,Liang Liu,Dongling Dai,Mingguo Xu

EMBO molecular medicine 15:e16431 PubMed37485814

2023

Actionable loss of SLF2 drives B-cell lymphomagenesis and impairs the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Le Zhang,Matthias Wirth,Upayan Patra,Jacob Stroh,Konstandina Isaakidis,Leonie Rieger,Susanne Kossatz,Maja Milanovic,Chuanbing Zang,Uta Demel,Jan Keiten-Schmitz,Kristina Wagner,Katja Steiger,Roland Rad,Florian Bassermann,Stefan Müller,Ulrich Keller,Markus Schick

Cell reports 32:108146 PubMed32937131

2020

Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ilan Theurillat,Ivo A Hendriks,Jack-Christophe Cossec,Alexandra Andrieux,Michael L Nielsen,Anne Dejean

Nature communications 10:4452 PubMed31575873

2019

The ubiquitin-like modifier FAT10 interferes with SUMO activation.

Applications

Unspecified application

Species

Unspecified reactive species

Annette Aichem,Carolin Sailer,Stella Ryu,Nicola Catone,Nicolas Stankovic-Valentin,Gunter Schmidtke,Frauke Melchior,Florian Stengel,Marcus Groettrup

Molecular cell 75:823-834.e5 PubMed31302001

2019

SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Tianshi Wang,Ying Cao,Quan Zheng,Jun Tu,Wei Zhou,Jianli He,Jie Zhong,Yalan Chen,Jiqiu Wang,Rong Cai,Yong Zuo,Bo Wei,Qiuju Fan,Jie Yang,Yicheng Wu,Jing Yi,Dali Li,Mingyao Liu,Chuangui Wang,Aiwu Zhou,Yu Li,Xuefeng Wu,Wen Yang,Y Eugene Chin,Guoqiang Chen,Jinke Cheng

Nucleic acids research 46:7108-7123 PubMed29893976

2018

Regulation of miR-34b/c-targeted gene expression program by SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Jia Li,Li Du,Grace Aldana-Masangkay,Xiuli Wang,Ryan Urak,Stephen J Forman,Steven T Rosen,Yuan Chen
View all publications
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