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AB185552

Anti-SAE1 antibody [EPR15398]

5

(1 Review)

|

(10 Publications)

Rabbit Recombinant Monoclonal SAE1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 10 publications.

View Alternative Names

AOS1, SUA1, UBLE1A, SAE1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (AB185552)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (AB185552)

Immunofluorescence analysis of MCF-7 cells labeling SAE1 with ab185552 at 1/500 dilution. Secondary ab : Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative : 4% paraformaldehyde. Counter stain : Dapi.

Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (AB185552)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (AB185552)

Immunofluorescence analysis of HeLa cells labeling SAE1 with ab185552 at 1/500 dilution. Secondary ab : Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative : 4% paraformaldehyde. Counter stain : Dapi.

Flow Cytometry (Intracellular) - Anti-SAE1 antibody [EPR15398] (AB185552)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SAE1 antibody [EPR15398] (AB185552)

Intracellular Flow Cytometry analysis of HeLa cells using ab185552 at 1/230 dilution. Secondary ab : Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative : 2% paraformaldehyde. Isotype control : Rabbit monoclonal IgG.

Immunoprecipitation - Anti-SAE1 antibody [EPR15398] (AB185552)
  • IP

Supplier Data

Immunoprecipitation - Anti-SAE1 antibody [EPR15398] (AB185552)

Immunoprecipitation from 293 cells using ab185552 at 1/50 dilution. Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. Blocking buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-SAE1 antibody [EPR15398] (ab185552)

Predicted band size: 38 kDa

false

Western blot - Anti-SAE1 antibody [EPR15398] (AB185552)
  • WB

Supplier Data

Western blot - Anti-SAE1 antibody [EPR15398] (AB185552)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SAE1 antibody [EPR15398] (ab185552) at 1/10000 dilution

Lane 1:

Hela cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

MCF-7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

  • Carrier free

    Anti-SAE1 antibody [EPR15398] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15398

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/230", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein SAE1 also known as SUMO-activating enzyme subunit 1 functions mechanically as a part of the SUMOylation process. It has a mass of approximately 39 kDa and is expressed in multiple cell types but is mainly found in the nucleus. SAE1 forms a heterodimeric structure with the protein SAE2 and together they are responsible for the initial step of SUMO activation which is important for SUMOylation a post-translational modification process.
Biological function summary

SAE1 is integral to numerous cellular processes that require precise regulation such as transcription DNA repair and signal transduction. It functions as part of the SUMO E1 enzyme complex which is essential for conjugating SUMO proteins to target substrates. This modification influences protein stability localization and interaction adding a layer of control in these cellular activities. The modulation of protein function by SAE1 impacts cell cycle control and stress responses indicating its breadth of influence across cellular mechanisms.

Pathways

The actions of SAE1 significantly impact the PI3K-Akt and DNA damage repair pathways. Its involvement in these pathways highlights its role in regulating cell survival and maintaining genomic integrity. SAE1's interaction within the SUMOylation machinery connects it to other proteins such as Ubc9 the E2 conjugating enzyme which highlights its importance in facilitating modifications necessary for a wide array of cellular functions and signaling cascades.

Defects or dysregulation of SAE1 have been linked to cancer and neurodegenerative conditions. Altered SUMOylation levels controlled by SAE1 can influence tumorigenesis through abnormal cell proliferation or impaired DNA damage responses connecting it with proteins like p53 and PARP1 which are critical in those pathways. Additionally SAE1's involvement in neurological disorders is associated with disrupted protein homeostasis linking it to pathologies where proper cellular regulatory mechanisms are compromised.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
See full target information SAE1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Respiratory research 26:275 PubMed41024125

2025

ZNF184-mediated transcriptional activation of SAE1 drives the cell cycle entry and immune evasion in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Shi,Luquan Zhang,Chunli Wang,Qingwei Meng

The breast journal 2024:2981722 PubMed39742381

2025

Upregulated SAE1 Drives Tumorigenesis and Is Associated with Poor Clinical Outcomes in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Liu,Jing Wang,Yunhai Li,Feng Luo,Lei Xing

Nature communications 14:3887 PubMed37393345

2023

SUMOylation of Rho-associated protein kinase 2 induces goblet cell metaplasia in allergic airways.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Tan,Meiping Lu,Yuqing Cai,Weibo Qi,Fugen Wu,Hangyang Bao,Meiyu Qv,Qiangqiang He,Yana Xu,Xiangzhi Wang,Tingyu Shen,Jiahao Luo,Yangxun He,Junsong Wu,Lanfang Tang,Muhammad Qasim Barkat,Chengyun Xu,Ximei Wu

BMC cancer 22:572 PubMed35606717

2022

Construction and validation of a two-gene signature based on SUMOylation regulatory genes in non-small cell lung cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxu Sheng,Zhexue Hao,Linhai Zhu,Yuan Zeng,Jianxing He

The Journal of biological chemistry 297:101200 PubMed34537242

2021

Linking nuclear matrix-localized PIAS1 to chromatin SUMOylation via direct binding of histones H3 and H2A.Z.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaosu Chen,Yunpeng Zhang,Qingqing Guan,Huifang Zhang,Jing Luo,Jialun Li,Wei Wei,Xiang Xu,Lujian Liao,Jiemin Wong,Jiwen Li

Frontiers in cell and developmental biology 9:667435 PubMed33996822

2021

The Absence of PTEN in Breast Cancer Is a Driver of MLN4924 Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Ge Du,Zhi-Qiang Peng,Wen-Bin Gai,Fan Liu,Wei Liu,Yu-Jiao Chen,Hong-Chang Li,Xin Zhang,Cui Hua Liu,Ling-Qiang Zhang,Hong Jiang,Ping Xie

Cells 10: PubMed33477333

2021

SUMO-Activating Enzyme Subunit 1 (SAE1) Is a Promising Diagnostic Cancer Metabolism Biomarker of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiann Ruey Ong,Oluwaseun Adebayo Bamodu,Nguyen Viet Khang,Yen-Kuang Lin,Chi-Tai Yeh,Wei-Hwa Lee,Yih-Giun Cherng

The Journal of biological chemistry 295:6741-6753 PubMed32249212

2020

SUMOylation of the transcription factor ZFHX3 at Lys-2806 requires SAE1, UBC9, and PIAS2 and enhances its stability and function in cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wu,Jiali Fang,Mingcheng Liu,Jun A,Jinming Liu,Wenxuan Chen,Juan Li,Gui Ma,Zhiqian Zhang,Baotong Zhang,Liya Fu,Jin-Tang Dong

Cell communication and signaling : CCS 17:82 PubMed31345225

2019

SAE1 promotes human glioma progression through activating AKT SUMOylation-mediated signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yanfang Yang,Ziwei Liang,Zijing Xia,Xixi Wang,Yanni Ma,Zenghua Sheng,Qingjia Gu,Guobo Shen,Liangxue Zhou,Hongxia Zhu,Ningzhi Xu,Shufang Liang

Osteoarthritis and cartilage 24:1113-24 PubMed26826302

2016

Hypoxia regulates sumoylation pathways in intervertebral disc cells: implications for hypoxic adaptations.

Applications

Unspecified application

Species

Unspecified reactive species

F Wang,F Cai,R Shi,J-N Wei,X-T Wu
View all publications
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