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Rabbit Polyclonal SAE1 phospho S185 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SUMO-activating enzyme subunit 1 phospho S185.

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Images

Western blot - Anti-SAE1 (phospho S185) antibody (AB106096), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human SUMO-activating enzyme subunit 1 phospho S185. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBE0

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted
Chimpanzee
Predicted
Cow
Predicted
Dog
Predicted

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Cow, Dog, Chimpanzee

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Alternative names

Recommended products

Rabbit Polyclonal SAE1 phospho S185 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SUMO-activating enzyme subunit 1 phospho S185.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human SUMO-activating enzyme subunit 1 phospho S185. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBE0
Purification technique

Affinity purification

Specificity

This purified antibody is directed against human SUMO Activating Enzyme E1 protein. A BLAST analysis using the sequence of the immunizing peptide was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%), bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.

Concentration
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Purification notes

This purified antibody is directed against human SUMO Activating Enzyme E1 protein. The product was purified from monospecific antiserum by affinity chromatography.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein SAE1 also known as SUMO-activating enzyme subunit 1 functions mechanically as a part of the SUMOylation process. It has a mass of approximately 39 kDa and is expressed in multiple cell types but is mainly found in the nucleus. SAE1 forms a heterodimeric structure with the protein SAE2 and together they are responsible for the initial step of SUMO activation which is important for SUMOylation a post-translational modification process.

Biological function summary

SAE1 is integral to numerous cellular processes that require precise regulation such as transcription DNA repair and signal transduction. It functions as part of the SUMO E1 enzyme complex which is essential for conjugating SUMO proteins to target substrates. This modification influences protein stability localization and interaction adding a layer of control in these cellular activities. The modulation of protein function by SAE1 impacts cell cycle control and stress responses indicating its breadth of influence across cellular mechanisms.

Pathways

The actions of SAE1 significantly impact the PI3K-Akt and DNA damage repair pathways. Its involvement in these pathways highlights its role in regulating cell survival and maintaining genomic integrity. SAE1's interaction within the SUMOylation machinery connects it to other proteins such as Ubc9 the E2 conjugating enzyme which highlights its importance in facilitating modifications necessary for a wide array of cellular functions and signaling cascades.

Associated diseases and disorders

Defects or dysregulation of SAE1 have been linked to cancer and neurodegenerative conditions. Altered SUMOylation levels controlled by SAE1 can influence tumorigenesis through abnormal cell proliferation or impaired DNA damage responses connecting it with proteins like p53 and PARP1 which are critical in those pathways. Additionally SAE1's involvement in neurological disorders is associated with disrupted protein homeostasis linking it to pathologies where proper cellular regulatory mechanisms are compromised.

Product promise

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1 product image

  • Western blot - Anti-SAE1 (phospho S185) antibody (ab106096), expandable thumbnail

    Western blot - Anti-SAE1 (phospho S185) antibody (ab106096)

    10% SDS-PAGE.
    Lower panel shows tubulin loading control

    All lanes: Western blot - Anti-SAE1 (phospho S185) antibody (ab106096) at 1/1000 dilution

    Lane 1: HeLa whole cell protein at 20 µg

    Lane 2: HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail at 20 µg

    Secondary

    All lanes: HRP-conjugated secondary

    Developed using the ECL technique.

    Predicted band size: 38 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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