Rabbit Polyclonal SAE1 phospho S185 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SUMO-activating enzyme subunit 1 phospho S185.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Mouse | Predicted |
Rat | Predicted |
Chimpanzee | Predicted |
Cow | Predicted |
Dog | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Dog, Chimpanzee | Dilution info - | Notes - |
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The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
AOS1, SUA1, UBLE1A, SAE1, AOS1, UBLE1A, SUA1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A
Rabbit Polyclonal SAE1 phospho S185 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SUMO-activating enzyme subunit 1 phospho S185.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate
Liquid
Polyclonal
Affinity purification
This purified antibody is directed against human SUMO Activating Enzyme E1 protein. A BLAST analysis using the sequence of the immunizing peptide was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%), bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.
This purified antibody is directed against human SUMO Activating Enzyme E1 protein. The product was purified from monospecific antiserum by affinity chromatography.
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The protein SAE1 also known as SUMO-activating enzyme subunit 1 functions mechanically as a part of the SUMOylation process. It has a mass of approximately 39 kDa and is expressed in multiple cell types but is mainly found in the nucleus. SAE1 forms a heterodimeric structure with the protein SAE2 and together they are responsible for the initial step of SUMO activation which is important for SUMOylation a post-translational modification process.
SAE1 is integral to numerous cellular processes that require precise regulation such as transcription DNA repair and signal transduction. It functions as part of the SUMO E1 enzyme complex which is essential for conjugating SUMO proteins to target substrates. This modification influences protein stability localization and interaction adding a layer of control in these cellular activities. The modulation of protein function by SAE1 impacts cell cycle control and stress responses indicating its breadth of influence across cellular mechanisms.
The actions of SAE1 significantly impact the PI3K-Akt and DNA damage repair pathways. Its involvement in these pathways highlights its role in regulating cell survival and maintaining genomic integrity. SAE1's interaction within the SUMOylation machinery connects it to other proteins such as Ubc9 the E2 conjugating enzyme which highlights its importance in facilitating modifications necessary for a wide array of cellular functions and signaling cascades.
Defects or dysregulation of SAE1 have been linked to cancer and neurodegenerative conditions. Altered SUMOylation levels controlled by SAE1 can influence tumorigenesis through abnormal cell proliferation or impaired DNA damage responses connecting it with proteins like p53 and PARP1 which are critical in those pathways. Additionally SAE1's involvement in neurological disorders is associated with disrupted protein homeostasis linking it to pathologies where proper cellular regulatory mechanisms are compromised.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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10% SDS-PAGE.
Lower panel shows tubulin loading control
All lanes: Western blot - Anti-SAE1 (phospho S185) antibody (ab106096) at 1/1000 dilution
Lane 1: HeLa whole cell protein at 20 µg
Lane 2: HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail at 20 µg
All lanes: HRP-conjugated secondary
Developed using the ECL technique.
Predicted band size: 38 kDa
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