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AB185955

Anti-SAE2 / UBA2 antibody [EPR14880]

4

(4 Reviews)

|

(17 Publications)

Rabbit Recombinant Monoclonal SAE2 / UBA2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 17 publications.

View Alternative Names

SAE2, UBLE1B, HRIHFB2115, UBA2, SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SAE2 / UBA2 with ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)

Immunofluorescent analysis of acetone-fixed HeLa cells labeling SAE2 / UBA2 with ab185955 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)
  • IP

Supplier Data

Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)

Western blot analysis of SAE2 / UBA2 in K562 cell lysate immunoprecipitated with ab185955 at 1/50 dilution.

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)

Predicted band size: 71 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling SAE2 / UBA2 with ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)
  • WB

Supplier Data

Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)

All lanes:

Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 71 kDa

Observed band size: 90 kDa

false

Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)
  • WB

Supplier Data

Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)

All lanes:

Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilution

Lane 1:

RAW 264.7 cell lysate at 10 µg

Lane 2:

PC-12 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 71 kDa

Observed band size: 90 kDa

false

  • Carrier free

    Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14880

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAE2 also known as UBA2 is a protein involved mechanically in the SUMO (Small Ubiquitin-like Modifier) conjugation pathway. It functions as an E1 activating enzyme collaborating closely with its partner SAE1 in the initiation of SUMOylation. SAE2 has a known molecular mass of approximately 72 kDa. It is widely expressed in various tissues with high expressions noted in the nucleus. This expression pattern suggests it plays a role in maintaining cellular processes under a variety of conditions.
Biological function summary

This protein plays an important role in the post-translational modification process of SUMOylation which is critical for regulating protein function. SAE2 is part of the E1 activating enzyme complex working specifically with SAE1 to activate and transfer SUMO to target proteins. It contributes to cellular processes such as nuclear-cytosolic transport chromosome segregation and DNA repair. This indicates its functions are central to maintaining genomic stability and cellular homeostasis.

Pathways

SAE2 is integral in the SUMOylation pathway which interacts with critical cellular pathways like the DNA damage response and cell cycle regulation pathways. It has important interactions with proteins such as UBE2I (also known as Ubc9) the only E2 enzyme in the SUMOylation cascade and PIAS family proteins which function as E3 ligases. These connections allow SAE2 to influence various pathways essential for cell survival and proliferation.

SAE2 influences pathological conditions such as cancer and neurodegenerative diseases. Its role in SUMOylation connects it to the regulation of oncogenic pathways potentially linking it with proteins like p53 a well-known tumor suppressor. In neurodegenerative diseases alterations in SUMOylation pathways with SAE2 as a participant might affect proteins involved in neuron function and survival contributing to disease progression. Understanding SAE2's interactions in these diseases might provide insights into therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
See full target information UBA2
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112555 PubMed40487451

2025

Targeting SUMOylation in ovarian cancer: Sensitivity, resistance, and the role of MYC.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha Littler,Bethany M Barnes,Rhys Owen,Louisa Nelson,Anthony Tighe,I-Hsuan Lin,Hugh C Osborne,Christine K Schmidt,Joanne C McGrail,Stephen S Taylor

Cell death & disease 15:674 PubMed39277582

2024

Impaired SUMOylation of FoxA1 promotes nonalcoholic fatty liver disease through down-regulation of Sirt6.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Zou,Jinwen Liao,Min Xiao,Liang Liu,Dongling Dai,Mingguo Xu

EMBO molecular medicine 15:e16431 PubMed37485814

2023

Actionable loss of SLF2 drives B-cell lymphomagenesis and impairs the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Le Zhang,Matthias Wirth,Upayan Patra,Jacob Stroh,Konstandina Isaakidis,Leonie Rieger,Susanne Kossatz,Maja Milanovic,Chuanbing Zang,Uta Demel,Jan Keiten-Schmitz,Kristina Wagner,Katja Steiger,Roland Rad,Florian Bassermann,Stefan Müller,Ulrich Keller,Markus Schick

Nature communications 14:3887 PubMed37393345

2023

SUMOylation of Rho-associated protein kinase 2 induces goblet cell metaplasia in allergic airways.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Tan,Meiping Lu,Yuqing Cai,Weibo Qi,Fugen Wu,Hangyang Bao,Meiyu Qv,Qiangqiang He,Yana Xu,Xiangzhi Wang,Tingyu Shen,Jiahao Luo,Yangxun He,Junsong Wu,Lanfang Tang,Muhammad Qasim Barkat,Chengyun Xu,Ximei Wu

BMC cancer 22:572 PubMed35606717

2022

Construction and validation of a two-gene signature based on SUMOylation regulatory genes in non-small cell lung cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxu Sheng,Zhexue Hao,Linhai Zhu,Yuan Zeng,Jianxing He

Science advances 8:eabm2960 PubMed35442725

2022

Epac1 activation by cAMP regulates cellular SUMOylation and promotes the formation of biomolecular condensates.

Applications

Unspecified application

Species

Unspecified reactive species

Wenli Yang,William G Robichaux,Fang C Mei,Wei Lin,Li Li,Sheng Pan,Mark A White,Yuan Chen,Xiaodong Cheng

Cell death & disease 12:1067 PubMed34753901

2021

Knockdown of ubiquitin-like modifier-activating enzyme 2 promotes apoptosis of clear cell renal cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guoxi Zhang,Junrong Zou,Jinglin Shi,Biao Qian,Kaiyang Qiu,Quanliang Liu,Tianpeng Xie,Zhihua He,Hui Xu,Yunfeng Liao,Yuting Wu,Yanmin Li,Guancheng Xiao,Yuanhu Yuan,Rihai Xiao,Gengqing Wu,Xiaofeng Zou

The Journal of biological chemistry 297:101200 PubMed34537242

2021

Linking nuclear matrix-localized PIAS1 to chromatin SUMOylation via direct binding of histones H3 and H2A.Z.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaosu Chen,Yunpeng Zhang,Qingqing Guan,Huifang Zhang,Jing Luo,Jialun Li,Wei Wei,Xiang Xu,Lujian Liao,Jiemin Wong,Jiwen Li

Journal of Cancer 12:6145-6154 PubMed34539887

2021

FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanfang Cheng,Yajuan Wang,Yuanzun Cheng,Quanzhong Yang,Lejing Zhang,Zhaoxi Li,Jiancheng Cheng

Cell chemical biology 28:1394-1406.e10 PubMed33979648

2021

Targeting SUMOylation dependency in human cancer stem cells through a unique SAE2 motif revealed by chemical genomics.

Applications

Unspecified application

Species

Unspecified reactive species

Yannick D Benoit,Ryan R Mitchell,Wenliang Wang,Luca Orlando,Allison L Boyd,Borko Tanasijevic,Lili Aslostovar,Zoya Shapovalova,Meaghan Doyle,Christopher J Bergin,Kinga Vojnits,Fanny L Casado,Justin Di Lu,Deanna P Porras,Juan Luis García-Rodriguez,Jennifer Russell,Aïcha Zouggar,Angelique N Masibag,Cody Caba,Kalinka Koteva,Lakshmana K Kinthada,Jagdish Suresh Patel,Sara N Andres,Jakob Magolan,Tony J Collins,Gerard D Wright,Mickie Bhatia
View all publications
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Product promise

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