Rabbit Recombinant Monoclonal SAE2 / UBA2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 14 publications.
Images
![Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-ab185955--western-blot-img105984.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)")
![Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-ab185955--western-blot-img54043.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-ab185955--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129707.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-ab185955--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79624.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)")
![Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-ab185955--immunoprecipitation-img90112.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)")
Publications
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- Nature communications 14:38872023SUMOylation of Rho-associated protein kinase 2 induces goblet cell metaplasia in allergic airways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Tan,Meiping Lu,Yuqing Cai,Weibo Qi,Fugen Wu,Hangyang Bao,Meiyu Qv,Qiangqiang He,Yana Xu,Xiangzhi Wang,Tingyu Shen,Jiahao Luo,Yangxun He,Junsong Wu,Lanfang Tang,Muhammad Qasim Barkat,Chengyun Xu,Ximei WuPubMed 37393345
- BMC cancer 22:5722022Construction and validation of a two-gene signature based on SUMOylation regulatory genes in non-small cell lung cancer patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongxu Sheng,Zhexue Hao,Linhai Zhu,Yuan Zeng,Jianxing HePubMed 35606717
- Science advances 8:eabm29602022Epac1 activation by cAMP regulates cellular SUMOylation and promotes the formation of biomolecular condensates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenli Yang,William G Robichaux,Fang C Mei,Wei Lin,Li Li,Sheng Pan,Mark A White,Yuan Chen,Xiaodong ChengPubMed 35442725
- Cell death & disease 12:10672021Knockdown of ubiquitin-like modifier-activating enzyme 2 promotes apoptosis of clear cell renal cell carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoxi Zhang,Junrong Zou,Jinglin Shi,Biao Qian,Kaiyang Qiu,Quanliang Liu,Tianpeng Xie,Zhihua He,Hui Xu,Yunfeng Liao,Yuting Wu,Yanmin Li,Guancheng Xiao,Yuanhu Yuan,Rihai Xiao,Gengqing Wu,Xiaofeng ZouPubMed 34753901
- The Journal of biological chemistry 297:1012002021Linking nuclear matrix-localized PIAS1 to chromatin SUMOylation via direct binding of histones H3 and H2A.Z.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaosu Chen,Yunpeng Zhang,Qingqing Guan,Huifang Zhang,Jing Luo,Jialun Li,Wei Wei,Xiang Xu,Lujian Liao,Jiemin Wong,Jiwen LiPubMed 34537242
- Journal of Cancer 12:6145-61542021FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanfang Cheng,Yajuan Wang,Yuanzun Cheng,Quanzhong Yang,Lejing Zhang,Zhaoxi Li,Jiancheng ChengPubMed 34539887
- Cell chemical biology 28:1394-1406.e102021Targeting SUMOylation dependency in human cancer stem cells through a unique SAE2 motif revealed by chemical genomics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYannick D Benoit,Ryan R Mitchell,Wenliang Wang,Luca Orlando,Allison L Boyd,Borko Tanasijevic,Lili Aslostovar,Zoya Shapovalova,Meaghan Doyle,Christopher J Bergin,Kinga Vojnits,Fanny L Casado,Justin Di Lu,Deanna P Porras,Juan Luis García-Rodriguez,Jennifer Russell,Aïcha Zouggar,Angelique N Masibag,Cody Caba,Kalinka Koteva,Lakshmana K Kinthada,Jagdish Suresh Patel,Sara N Andres,Jakob Magolan,Tony J Collins,Gerard D Wright,Mickie BhatiaPubMed 33979648
- Animal science journal = Nihon chikusan Gakkaiho 92:e135482021Effects of the E1 activating enzyme UBA2 on porcine oocyte maturation, apoptosis, and embryonic development in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDa Xu,Jing Bi,Yunfeng Guan,Xiaotong Luo,Xuan Chen,Yanqiu Lv,Yi JinPubMed 33835647
- Cell reports 32:1081462020Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlan Theurillat,Ivo A Hendriks,Jack-Christophe Cossec,Alexandra Andrieux,Michael L Nielsen,Anne DejeanPubMed 32937131
- Nature communications 10:44522019The ubiquitin-like modifier FAT10 interferes with SUMO activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnette Aichem et. al.PubMed 31575873
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Tested |
| Rat | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 - 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 - 1/2000 | Notes - |
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Alternative names
SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2, HRIHFB2115, UBLE1B, SAE2, UBA2
Recommended products
Rabbit Recombinant Monoclonal SAE2 / UBA2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 14 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR14880
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-ab185955--western-blot-img105984.jpg "Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)")
Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
All lanes: Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: A549 cell lysate at 20 µg
Lane 3: K562 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 90 kDa
![Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-ab185955--western-blot-img54043.jpg "Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)")
Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
All lanes: Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilution
Lane 1: RAW 264.7 cell lysate at 10 µg
Lane 2: PC-12 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 90 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-ab185955--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129707.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SAE2 / UBA2 with ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-ab185955--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79624.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling SAE2 / UBA2 with ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-ab185955--immunoprecipitation-img90112.jpg "Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)")
Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
Western blot analysis of SAE2 / UBA2 in K562 cell lysate immunoprecipitated with ab185955 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
Predicted band size: 71 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-ab185955--immunocytochemistry-immunofluorescence-img17282.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)")
Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
Immunofluorescent analysis of acetone-fixed HeLa cells labeling SAE2 / UBA2 with ab185955 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-ab185955--immunocytochemistry-immunofluorescence-img17282.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)")
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