Rabbit Recombinant Monoclonal SAE2 / UBA2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (AB250858), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-bsa-and-azide-free-ab250858--western-blot-img105984.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (AB250858)")
![Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (AB250858), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-bsa-and-azide-free-ab250858--western-blot-img54043.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (AB250858)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (AB250858), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-bsa-and-azide-free-ab250858--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129707.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (AB250858)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (AB250858), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-bsa-and-azide-free-ab250858--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79624.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (AB250858)")
![Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (AB250858), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-bsa-and-azide-free-ab250858--immunoprecipitation-img90112.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (AB250858)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Tested |
| Rat | Expected | Expected | Tested | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Alternative names
SAE2, UBLE1B, HRIHFB2115, UBA2, SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2
Recommended products
Rabbit Recombinant Monoclonal SAE2 / UBA2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR14880
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250858 is the carrier-free version of Anti-SAE2 / UBA2 antibody [EPR14880] ab185955.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SAE2 also known as UBA2 is a protein involved mechanically in the SUMO (Small Ubiquitin-like Modifier) conjugation pathway. It functions as an E1 activating enzyme collaborating closely with its partner SAE1 in the initiation of SUMOylation. SAE2 has a known molecular mass of approximately 72 kDa. It is widely expressed in various tissues with high expressions noted in the nucleus. This expression pattern suggests it plays a role in maintaining cellular processes under a variety of conditions.
- Biological function summary
This protein plays an important role in the post-translational modification process of SUMOylation which is critical for regulating protein function. SAE2 is part of the E1 activating enzyme complex working specifically with SAE1 to activate and transfer SUMO to target proteins. It contributes to cellular processes such as nuclear-cytosolic transport chromosome segregation and DNA repair. This indicates its functions are central to maintaining genomic stability and cellular homeostasis.
- Pathways
SAE2 is integral in the SUMOylation pathway which interacts with critical cellular pathways like the DNA damage response and cell cycle regulation pathways. It has important interactions with proteins such as UBE2I (also known as Ubc9) the only E2 enzyme in the SUMOylation cascade and PIAS family proteins which function as E3 ligases. These connections allow SAE2 to influence various pathways essential for cell survival and proliferation.
- Associated diseases and disorders
SAE2 influences pathological conditions such as cancer and neurodegenerative diseases. Its role in SUMOylation connects it to the regulation of oncogenic pathways potentially linking it with proteins like p53 a well-known tumor suppressor. In neurodegenerative diseases alterations in SUMOylation pathways with SAE2 as a participant might affect proteins involved in neuron function and survival contributing to disease progression. Understanding SAE2's interactions in these diseases might provide insights into therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-bsa-and-azide-free-ab250858--western-blot-img105984.jpg "Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)")
Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)
This data was developed using Anti-SAE2 / UBA2 antibody [EPR14880] ab185955, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (Anti-SAE2 / UBA2 antibody [EPR14880] ab185955) at 1/1000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: A549 cell lysate at 20 µg
Lane 3: K562 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 90 kDa
![Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-bsa-and-azide-free-ab250858--western-blot-img54043.jpg "Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)")
Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)
This data was developed using Anti-SAE2 / UBA2 antibody [EPR14880] ab185955, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SAE2 / UBA2 antibody [EPR14880] (Anti-SAE2 / UBA2 antibody [EPR14880] ab185955) at 1/1000 dilution
Lane 1: RAW 264.7 cell lysate at 10 µg
Lane 2: PC-12 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 90 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-bsa-and-azide-free-ab250858--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129707.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)
This data was developed using Anti-SAE2 / UBA2 antibody [EPR14880] ab185955, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SAE2 / UBA2 with Anti-SAE2 / UBA2 antibody [EPR14880] ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-bsa-and-azide-free-ab250858--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79624.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)
This data was developed using Anti-SAE2 / UBA2 antibody [EPR14880] ab185955, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling SAE2 / UBA2 with Anti-SAE2 / UBA2 antibody [EPR14880] ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-bsa-and-azide-free-ab250858--immunoprecipitation-img90112.jpg "Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)")
Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)
This data was developed using Anti-SAE2 / UBA2 antibody [EPR14880] ab185955, the same antibody clone in a different buffer formulation.Western blot analysis of SAE2 / UBA2 in K562 cell lysate immunoprecipitated with Anti-SAE2 / UBA2 antibody [EPR14880] ab185955 at 1/50 dilution.Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-SAE2 / UBA2 antibody [EPR14880] (Anti-SAE2 / UBA2 antibody [EPR14880] ab185955)
Predicted band size: 71 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-bsa-and-azide-free-ab250858--immunocytochemistry-immunofluorescence-img17282.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)")
Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)
This data was developed using Anti-SAE2 / UBA2 antibody [EPR14880] ab185955, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of acetone-fixed HeLa cells labeling SAE2 / UBA2 with Anti-SAE2 / UBA2 antibody [EPR14880] ab185955 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858), expandable thumbnail](https://content.abcam.com/products/images/sae2-uba2-antibody-epr14880-bsa-and-azide-free-ab250858--immunocytochemistry-immunofluorescence-img17282.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [EPR14880] - BSA and Azide free (ab250858)")
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