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AB104220

Anti-SAF-B2 antibody

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(1 Publication)

Rabbit Polyclonal SAF-B2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SAFB2 aa 350-550.

View Alternative Names

KIAA0138, SAFB2, Scaffold attachment factor B2, SAF-B2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SAF-B2 antibody (AB104220)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SAF-B2 antibody (AB104220)

Immunofluorescence analysis of methanol-fixed A431, using ab104220 at 1/200 dilution. Lower picture costained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAF-B2 antibody (AB104220)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAF-B2 antibody (AB104220)

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using ab104220 at 1/100 dilution.

Western blot - Anti-SAF-B2 antibody (AB104220)
  • WB

Unknown

Western blot - Anti-SAF-B2 antibody (AB104220)

7.5% SDS PAGE

All lanes:

Western blot - Anti-SAF-B2 antibody (ab104220) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 107 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SAFB2 aa 350-550. The exact immunogen used to generate this antibody is proprietary information.

Q14151

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer ( PH8.0).</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAF-B2 also known as Scaffold Attachment Factor B2 is a critical protein in cellular regulation with a molecular weight of approximately 100 kDa. It is widely expressed in the nucleus of various cell types serving a role in transcriptional regulation. As a component of the scaffold/matrix attachment regions (S/MARs) it interacts with chromosomal DNA to influence gene expression. This interaction helps in organizing chromosomal structures profoundly impacting gene accessibility and transcription processes.
Biological function summary

SAF-B2 takes part in regulating transcription and RNA processing contributing to its functional versatility. It interacts with several RNA-binding proteins as part of a nuclear protein complex. These interactions suggest SAF-B2’s involvement in RNA splicing and stabilization linking it to mRNA processing pathways. The protein plays an important role in maintaining transcriptional fidelity and ensuring appropriate cellular response to varying signals.

Pathways

SAF-B2 affects transcription and mRNA processing pathways significantly. It associates with proteins like SAF-B which also participate in chromatin remodeling and gene regulation. Both proteins contribute to the regulation of gene expression through their participation in the complex network of transcriptional pathways. Additionally SAF-B2's involvement in signaling pathways indicates its broader role in cellular communication and response modulation.

Alterations in SAF-B2 have connections to cancer and neurodegenerative diseases. Aberrant expression or mutation can disrupt normal gene regulation processes contributing to tumorigenesis or neural dysfunction. The protein's interaction with SAF-B highlights the potential for misregulated pathways to promote disease states. Understanding SAF-B2’s role in these processes aids in developing targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to scaffold/matrix attachment region (S/MAR) DNA. Can function as an estrogen receptor corepressor and can also inhibit cell proliferation.
See full target information SAFB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e18209 PubMed38682349

2024

Exosomal HSPB1, interacting with FUS protein, suppresses hypoxia-induced ferroptosis in pancreatic cancer by stabilizing Nrf2 mRNA and repressing P450.

Applications

Unspecified application

Species

Unspecified reactive species

Lun Zhang,Liuxu Yang,Keyuan Du
View all publications
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Product promise

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