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AB97520

Anti-SAFB antibody

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(1 Publication)

Rabbit Polyclonal SAFB antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SAFB aa 450-750.

View Alternative Names

HAP, HET, SAFB1, SAFB, Scaffold attachment factor B1, SAF-B, SAF-B1, HSP27 estrogen response element-TATA box-binding protein, HSP27 ERE-TATA-binding protein

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-SAFB antibody (AB97520)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SAFB antibody (AB97520)

ab97520, at a 1/100 dilution, staining SAFB in paraformaldehyde fixed HeLa cells by Immunofluorescence analysis.
Lower image was merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAFB antibody (AB97520)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAFB antibody (AB97520)

Immunohistochemical analysis of mouse esophagus tissue stained for Scaffold attachment factor B1 protein at nucleus with ab97520 at a 1/ dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAFB antibody (AB97520)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAFB antibody (AB97520)

Immunohistochemical analysis of mouse intestine tissue stained for Scaffold attachment factor B1 protein at nucleus with ab97520 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAFB antibody (AB97520)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAFB antibody (AB97520)

Immunohistochemical analysis of rat fore brain tissue stained for Scaffold attachment factor B1 protein at nucleus with ab97520 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAFB antibody (AB97520)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAFB antibody (AB97520)

Immunohistochemical analysis of mouse fore brain tissue stained for Scaffold attachment factor B1 protein at nucleus with ab97520 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SAFB aa 450-750. The exact immunogen used to generate this antibody is proprietary information.

Q15424

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAFB also known as Scaffold Attachment Factor B is a protein that participates in multiple cellular functions. It has a molecular weight of approximately 135 kDa. Researchers observe its expression in the nucleus of a variety of cell types including epithelial and fibroblast cells. In a mechanical context SAFB plays a role in binding to scaffold/matrix-attachment regions (S/MARs) of DNA influencing transcription and chromatin organization.
Biological function summary

SAFB works as a multifunctional protein that regulates gene expression and RNA processing. It acts as part of protein complexes such as those involved in modulating estrogen receptor activity. SAFB interacts with chromatin and RNA acting as a bridge between these important cellular components. It influences transcription by functioning as both a transcriptional repressor and activator depending on the context within the cell.

Pathways

Several cellular interactions highlight the role of SAFB within nuclear signaling networks like the estrogen signaling pathway and stress response pathways. SAFB associates with proteins such as estrogen receptor α (ERα) and heat shock proteins (HSP) in these pathways. Its interactions help modulate dynamics within these networks impacting cellular responses to hormonal and stress stimuli.

Researchers have connected SAFB with breast cancer and hormone-related conditions. In breast cancer SAFB's interaction with ERα suggests a role in cancer cell proliferation and response to hormonal therapy. Connections also exist with stress-response proteins like HSP70 indicating potential involvement in the cellular stress framework. Such relationships point towards SAFB's roles in disease progression and possible therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (PubMed : 9671816). Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription (PubMed : 12660241). Thereby acts as a negative regulator of cell proliferation (PubMed : 12660241). When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity).
See full target information SAFB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 9:20 PubMed21605409

2011

A facile nanoparticle immunoassay for cancer biomarker discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Huo,Jimmie Colon,Adam Cordero,Jelena Bogdanovic,Cheryl H Baker,Steven Goodison,Marianna Y Pensky
View all publications
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Product promise

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