Skip to main content

Rabbit Recombinant Monoclonal SAGE1 antibody. Suitable for IHC-P, mIHC and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody [EPR21747] (AB233388), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody [EPR21747] (AB233388), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody [EPR21747] (AB233388), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-SAGE1 antibody [EPR21747] (AB233388), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-SAGE1 antibody [EPR21747] (AB233388), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PmIHC
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Target data

Alternative names

SAGE, SAGE1, Sarcoma antigen 1, Cancer/testis antigen 14, CT14

Recommended products

Rabbit Recombinant Monoclonal SAGE1 antibody. Suitable for IHC-P, mIHC and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21747
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The SAGE1 protein also known as Sarcoma Antigen Gene 1 has a molecular weight of approximately 29 kDa. It is primarily expressed in the testis with occasional expression in certain tumor types. SAGE1 belongs to the cancer/testis antigen family indicating its role in reproductive or tumor-specific functions. Researchers find it predominantly expressed in various types of sarcomas and some cancers drawing attention to its role in oncological studies.

Biological function summary

The SAGE1 protein shows expression linked to cellular processes specific to testis development and potentially in tumorigenesis. It might be part of a complex influencing cellular differentiation and proliferation. Studies indicate that SAGE1 has roles in cell signaling pathways that help modulate gene expression. Researchers continue to investigate its involvement in linking normal physiological functions within testis tissues to aberrations seen in cancerous transformations.

Pathways

Research focuses on the involvement of SAGE1 in signaling pathways like the MAPK pathway and possibly the PI3K/AKT pathway. These pathways are significant in cell division and survival providing possible mechanisms where SAGE1 affects cellular behavior. The MAPK pathway associates SAGE1 with proteins like RAF and MEK while in the PI3K/AKT pathway proteins like AKT1 might interact reflecting the influence of SAGE1 on cell growth and survival.

Associated diseases and disorders

Researchers note significant correlations between SAGE1 expression and certain cancers particularly sarcomas and testicular cancer. The expression of SAGE1 in these conditions suggests it as a potential biomarker for diagnosis or treatment targets. In cancerous states the presence of SAGE1 connects with altered pathways involving proteins such as BCL2 which impacts cell apoptosis emphasizing its role in the pathology of these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody [EPR21747] (ab233388), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody [EPR21747] (ab233388)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling SAGE1 with ab233388 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human bladder cancer (PMID:10919659). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody [EPR21747] (ab233388), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody [EPR21747] (ab233388)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling SAGE1 with ab233388 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Negative control: No staining in human placenta (PMID: 26252478).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody [EPR21747] (ab233388), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody [EPR21747] (ab233388)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling SAGE1 with ab233388 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in spermatogonia of human testis (PMID:26252478). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Multiplex immunohistochemistry - Anti-SAGE1 antibody [EPR21747] (ab233388), expandable thumbnail

    Multiplex immunohistochemistry - Anti-SAGE1 antibody [EPR21747] (ab233388)

    Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human testis tissue sections labeling DKKL1 with Anti-DKKL1 antibody [EPR25721-68] ab316308 at a 1/2000 dilutions (B), Wilms Tumor Protein with Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free ab275970 at a 1/1200 dilutions (C), and SAGE1 with ab233388 at a 1/250 dilutions (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody.


    Panel A: merged staining of anti-DKKL1 (red; Opal™ 570), anti-Wilms Tumor Protein (green; Opal™ 520) and anti-SAGE1 (gray; Opal™ 690) on human testis.

    Panel B: anti-DKKL1 staining mature sperm cells in human testis.

    Panel C: anti-Wilms Tumor Protein staining sertoli cells in human testis.

    Panel D: anti-SAGE1 staining spermatogonia in human testis.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. The section was incubated in three rounds of staining: in the order of Anti-DKKL1 antibody [EPR25721-68] ab316308, Anti-Wilms Tumor Protein antibody [EPR23963-116] - BSA and Azide free ab275970, and ab233388 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    DAPI was used as a nuclear counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-SAGE1 antibody [EPR21747] (ab233388), expandable thumbnail

    Multiplex immunohistochemistry - Anti-SAGE1 antibody [EPR21747] (ab233388)

    Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human testis tissue.

    Panel A: Merged staining of anti-IGF2BP1/IMP1 (green; Opal™690), anti-TPTE (gray; Opal™520) and anti-SAGE1 (red; Opal™570) on human testis.
    Panel B: Anti-IGF2BP1/IMP1 stained on cytoplasm of spermatogonia.
    Panel C: Anti-TPTE stained on spermatocytes.
    Panel D: Anti-SAGE1 stained on nucleus of spermatogonia.

    The section was incubated in three rounds of staining: in the order of Anti-IGF2BP1/IMP1 antibody [EPR26408-18] ab290736, Anti-TPTE antibody [EPR11867(2)] - BSA and Azide free ab250148, and ab233388 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com