Anti-SAGE1 antibody [EPR21747]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody [EPR21747] (AB233388)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling SAGE1 with ab233388 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Negative control : No staining in human placenta (PMID : 26252478).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody [EPR21747] (AB233388)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling SAGE1 with ab233388 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human bladder cancer (PMID : 10919659). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAGE1 antibody [EPR21747] (AB233388)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling SAGE1 with ab233388 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in spermatogonia of human testis (PMID : 26252478). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SAGE1 antibody [EPR21747] (AB233388)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human testis tissue. Panel A : Merged staining of anti-IGF2BP1/IMP1 (green; Opal™690), anti-TPTE (gray; Opal™520) and anti-SAGE1 (red; Opal™570) on human testis. Panel B : Anti-IGF2BP1/IMP1 stained on cytoplasm of spermatogonia. Panel C : Anti-TPTE stained on spermatocytes. Panel D : Anti-SAGE1 stained on nucleus of spermatogonia. The section was incubated in three rounds of staining : in the order of ab290736, ab250148, and ab233388 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-SAGE1 antibody [EPR21747] (AB233388)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human testis tissue sections labeling DKKL1 with ab316308 at a 1/2000 dilutions (B), Wilms Tumor Protein with ab275970 at a 1/1200 dilutions (C), and SAGE1 with ab233388 at a 1/250 dilutions (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-DKKL1 (red; Opal™ 570), anti-Wilms Tumor Protein (green; Opal™ 520) and anti-SAGE1 (gray; Opal™ 690) on human testis.
Panel B : anti-DKKL1 staining mature sperm cells in human testis.
Panel C : anti-Wilms Tumor Protein staining sertoli cells in human testis.
Panel D : anti-SAGE1 staining spermatogonia in human testis.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. The section was incubated in three rounds of staining : in the order of ab316308, ab275970, and ab233388 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

DAPI was used as a nuclear counterstain.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.