AB134966

Anti-SAHH antibody [EPR9261]

RabMAb
Recombinant
What is this?

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(9 Publications)

Rabbit Recombinant Monoclonal Adenosyl Homocysteine antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications.

View Alternative Names

SAHH, AHCY, Adenosylhomocysteinase, AdoHcyase, S-adenosyl-L-homocysteine hydrolase

1 Images

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Western blot - Anti-SAHH antibody [EPR9261] (AB134966)

All lanes:

Western blot - Anti-SAHH antibody [EPR9261] (ab134966) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 47 kDa

false

Carrier free

Anti-SAHH antibody [EPR9261] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9261

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S-Adenosylhomocysteine hydrolase also known as SAHH plays a critical mechanical role in cellular metabolism. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to homocysteine and adenosine. This enzyme approximately 48 kDa in mass is ubiquitously expressed in most tissues highlighting its universal importance in biological systems. SAHH maintains the cellular methylation balance by regulating the concentrations of SAH a byproduct of methylation reactions.

Biological function summary

S-Adenosylhomocysteine hydrolase acts as an important player in the methylation processes within the cell. By breaking down SAH it ensures proper methylation of DNA proteins and lipids through the recycling of homocysteine and adenosine. SAHH operates as a homotetramer forming a complex essential for its enzymatic function. The balance maintained by SAHH in cellular methylation allows for proper gene expression and signal transduction within various cellular processes.

Pathways

S-Adenosylhomocysteine hydrolase plays an essential role in the methionine cycle and one-carbon metabolism pathways. These pathways are interconnected with the folate cycle important for nucleotide synthesis and cellular methylation. SAHH works with enzymes like methionine synthase and adenosylmethionine linking it to regulation of homocysteine levels. These interconnections are fundamental for maintaining cellular function and homeostasis.

S-Adenosylhomocysteine hydrolase dysregulation can have significant implications. Hyperhomocysteinemia a condition characterized by high levels of homocysteine in the blood often results from SAHH dysfunction leading to cardiovascular diseases. Additionally aberrant SAHH activity connects to certain types of cancer as altered methylation patterns can disrupt normal cell proliferation. The interplay of SAHH with other proteins such as methylenetetrahydrofolate reductase (MTHFR) illustrates the complex network within which this enzyme operates affecting disease outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed : 10933798). Binds copper ions (By similarity).

See full target information AHCY

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:108852 PubMed38303706

2024

Ultrasound-targeted microbubble technology facilitates SAHH gene delivery to treat diabetic cardiomyopathy by activating AMPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Guo,Kegong Chen,Lin Ji,Shanjie Wang,Xiangmei Ye,Liang Xu,Leiguang Feng

International journal of molecular sciences 24: PubMed38003292

2023

Effects of S-Adenosylhomocysteine Hydrolase Downregulation on Wnt Signaling Pathway in SW480 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Pavičić,Filip Rokić,Oliver Vugrek

Bioengineered 12:10073-10088 PubMed34709988

2021

Screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liru Zhang,Huanju Li,Yongle Qiu,Yuanhang Liu,Xin Liu,Wenjing Wang

Cell reports 36:109487 PubMed34348140

2021

Ketogenesis impact on liver metabolism revealed by proteomics of lysine β-hydroxybutyrylation.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin B Koronowski,Carolina M Greco,He Huang,Jin-Kwang Kim,Jennifer L Fribourgh,Priya Crosby,Lavina Mathur,Xuelian Ren,Carrie L Partch,Cholsoon Jang,Feng Qiao,Yingming Zhao,Paolo Sassone-Corsi

Science advances 6: PubMed33328229

2020

S-adenosyl-l-homocysteine hydrolase links methionine metabolism to the circadian clock and chromatin remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Magdalen Greco,Marlene Cervantes,Jean-Michel Fustin,Kakeru Ito,Nicholas Ceglia,Muntaha Samad,Jiejun Shi,Kevin Brian Koronowski,Ignasi Forne,Suman Ranjit,Jonathan Gaucher,Kenichiro Kinouchi,Rika Kojima,Enrico Gratton,Wei Li,Pierre Baldi,Axel Imhof,Hitoshi Okamura,Paolo Sassone-Corsi

Nature metabolism 2:1062-1076 PubMed32747794

2020

Polyamine pathway activity promotes cysteine essentiality in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Zhang,Christin Bauer,Alice C Newman,Alejandro Huerta Uribe,Dimitris Athineos,Karen Blyth,Oliver D K Maddocks

Proceedings of the National Academy of Sciences of 117:7755-7763 PubMed32193337

2020

O-GlcNAcylation regulates the methionine cycle to promote pluripotency of stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Zhu,Xuejun Cheng,Yaxian Cheng,Junchen Chen,Huan Xu,Yuntao Gao,Xiaotao Duan,Junfeng Ji,Xuekun Li,Wen Yi

Scientific reports 8:14012 PubMed30228286

2018

Knock-down of AHCY and depletion of adenosine induces DNA damage and cell cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Lucija Belužić,Ivana Grbeša,Robert Belužić,Jong Hoon Park,Hyun Kyung Kong,Nevenka Kopjar,Guadalupe Espadas,Eduard Sabidó,Adriana Lepur,Filip Rokić,Ivanka Jerić,Lidija Brkljačić,Oliver Vugrek

American journal of cancer research 5:2127-38 PubMed26328244

2015

Inhibition of S-adenosylhomocysteine hydrolase decreases cell mobility and cell proliferation through cell cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Sae Jeong Park,Hyun Kyung Kong,Ye Sol Kim,Yeon Seon Lee,Jong Hoon Park

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SAHH antibody [EPR9261]

Western blot - Anti-SAHH antibody [EPR9261] (AB134966)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise