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AB248758

Anti-SAHH antibody [EPR9261] - BSA and Azide free

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Rabbit Recombinant Monoclonal Adenosyl Homocysteine antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

SAHH, AHCY, Adenosylhomocysteinase, AdoHcyase, S-adenosyl-L-homocysteine hydrolase

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Western blot - Anti-SAHH antibody [EPR9261] - BSA and Azide free (AB248758)
  • WB

Unknown

Western blot - Anti-SAHH antibody [EPR9261] - BSA and Azide free (AB248758)

This data was developed using ab134966, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SAHH antibody [EPR9261] (<a href='/en-us/products/primary-antibodies/sahh-antibody-epr9261-ab134966'>ab134966</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 47 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9261

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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AB309446

Human SAHH ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S-Adenosylhomocysteine hydrolase also known as SAHH plays a critical mechanical role in cellular metabolism. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to homocysteine and adenosine. This enzyme approximately 48 kDa in mass is ubiquitously expressed in most tissues highlighting its universal importance in biological systems. SAHH maintains the cellular methylation balance by regulating the concentrations of SAH a byproduct of methylation reactions.
Biological function summary

S-Adenosylhomocysteine hydrolase acts as an important player in the methylation processes within the cell. By breaking down SAH it ensures proper methylation of DNA proteins and lipids through the recycling of homocysteine and adenosine. SAHH operates as a homotetramer forming a complex essential for its enzymatic function. The balance maintained by SAHH in cellular methylation allows for proper gene expression and signal transduction within various cellular processes.

Pathways

S-Adenosylhomocysteine hydrolase plays an essential role in the methionine cycle and one-carbon metabolism pathways. These pathways are interconnected with the folate cycle important for nucleotide synthesis and cellular methylation. SAHH works with enzymes like methionine synthase and adenosylmethionine linking it to regulation of homocysteine levels. These interconnections are fundamental for maintaining cellular function and homeostasis.

S-Adenosylhomocysteine hydrolase dysregulation can have significant implications. Hyperhomocysteinemia a condition characterized by high levels of homocysteine in the blood often results from SAHH dysfunction leading to cardiovascular diseases. Additionally aberrant SAHH activity connects to certain types of cancer as altered methylation patterns can disrupt normal cell proliferation. The interplay of SAHH with other proteins such as methylenetetrahydrofolate reductase (MTHFR) illustrates the complex network within which this enzyme operates affecting disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed : 10933798). Binds copper ions (By similarity).
See full target information AHCY
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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