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Rabbit Recombinant Monoclonal AMY1A antibody. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (AB209401), expandable thumbnail
  • Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (AB209401), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308). Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308).

Alternative names

AMY1, AMY1A, Alpha-amylase 1A, Salivary alpha-amylase

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Rabbit Recombinant Monoclonal AMY1A antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18070
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (ab209401), expandable thumbnail

    Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (ab209401)

    Salivary alpha amylase Western blot staining using rabbit Anti-Salivary alpha amylase antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time : Lane 1: 5 seconds; Lane 2/3/4/5: 3 minutes.

    All lanes: Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (ab209401) at 1/2000 dilution

    Lane 1: Human pancreas lysate at 10 µg

    Lane 2: Human fetal brain lysate at 10 µg

    Lane 3: Human fetal heart lysate at 10 µg

    Lane 4: Human fetal kidney lysate at 10 µg

    Lane 5: Human fetal spleen lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 58 kDa

    Observed band size: 58 kDa

  • Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (ab209401), expandable thumbnail

    Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (ab209401)

    Salivary alpha amylase Western blot staining using rabbit Anti-Salivary alpha amylase antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (ab209401) at 1/5000 dilution

    Lane 1: Human pancreas lysate (control) at 10 µg

    Lane 2: Human pancreas lysate using AMY1 peptide block at 10 µg

    Lane 3: Human pancreas lysate using AMYP(AMY2B) peptide block at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 58 kDa

    Observed band size: 58 kDa

    Exposure time: 3s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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