AB209401

Anti-Salivary alpha amylase antibody [EPR18070]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal AMY1A antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AMY1, AMY1A, Alpha-amylase 1A, Salivary alpha-amylase

2 Images

Unknown

Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (AB209401)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (ab209401) at 1/5000 dilution

Lane 1:

Human pancreas lysate (control) at 10 µg

Lane 2:

Human pancreas lysate using AMY1 peptide block at 10 µg

Lane 3:

Human pancreas lysate using AMYP(AMY2B) peptide block at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

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Exposure time: 3s

Supplier Data

Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (AB209401)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 5 seconds; Lane 2/3/4/5 : 3 minutes.

All lanes:

Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (ab209401) at 1/2000 dilution

Lane 1:

Human pancreas lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

Human fetal kidney lysate at 10 µg

Lane 5:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

false

Carrier free

Anti-Salivary alpha amylase antibody [EPR18070] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18070

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed : 12527308). Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed : 12527308).

See full target information AMY1A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 8:1034 PubMed29044119

2017

The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ian J Huggins,Tomas Bos,Olivia Gaylord,Christina Jessen,Brianna Lonquich,Angeline Puranen,Jenna Richter,Charlotte Rossdam,David Brafman,Terry Gaasterland,Karl Willert

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Salivary alpha amylase antibody [EPR18070]

Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (AB209401)

Western blot - Anti-Salivary alpha amylase antibody [EPR18070] (AB209401)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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Product promise